Book contents
- Principles of IVF Laboratory Practice
- Principles of IVF Laboratory Practice
- Copyright page
- Contents
- Contributors
- Foreword
- The Evolution of IVF Practice
- Section 1Starting a New Laboratory and Training Protocols
- Section 2Pre-procedure Protocols
- Section 3Gametes
- Section 4Insemination/ICSI
- Chapter 21General Insemination for IVF
- Chapter 22Conventional IVF with Short Co-incubation
- Chapter 23Technical Aspects of ICSI for Ejaculated Spermatozoa
- Chapter 24Set-Up and Adapted Protocol for Piezo-ICSI
- Chapter 25Sperm Selection for ICSI by Morphology
- Chapter 26Sperm Selection for ICSI by Viability
- Chapter 27Artificial Oocyte Activation for IVF
- Section 5Fertilization Assessment
- Section 6Embryo Assessment: Morphology and Beyond
- Section 7Embryo Cryopreservation
- Section 8Embryo Transfer
- Section 9Quality Management
- Index
- References
Chapter 27 - Artificial Oocyte Activation for IVF
from Section 4 - Insemination/ICSI
Published online by Cambridge University Press: 07 August 2023
Book contents
- Principles of IVF Laboratory Practice
- Principles of IVF Laboratory Practice
- Copyright page
- Contents
- Contributors
- Foreword
- The Evolution of IVF Practice
- Section 1Starting a New Laboratory and Training Protocols
- Section 2Pre-procedure Protocols
- Section 3Gametes
- Section 4Insemination/ICSI
- Chapter 21General Insemination for IVF
- Chapter 22Conventional IVF with Short Co-incubation
- Chapter 23Technical Aspects of ICSI for Ejaculated Spermatozoa
- Chapter 24Set-Up and Adapted Protocol for Piezo-ICSI
- Chapter 25Sperm Selection for ICSI by Morphology
- Chapter 26Sperm Selection for ICSI by Viability
- Chapter 27Artificial Oocyte Activation for IVF
- Section 5Fertilization Assessment
- Section 6Embryo Assessment: Morphology and Beyond
- Section 7Embryo Cryopreservation
- Section 8Embryo Transfer
- Section 9Quality Management
- Index
- References
Summary
The implementation of intracytoplasmic sperm injection (ICSI) in the field of assisted reproduction has allowed fertilization in couples with previous fertilization failure. However, complete fertilization failure after ICSI shows a prevalence of up to 3%. Moreover, low or moderate fertilization (<30%) can be observed for some patients. In such cycles of bad prognosis, the underlying problem is most likely related to a failure of oocyte activation. In vivo, a sperm-derived enzyme mediates the physiological stimulus of the complex process of oocyte activation. In vitro, a complete fertilization failure can eventually be compensated by several artificial oocyte activation (AOA) techniques, all of which aim for an increase in internal Ca²⁺. AOA is only applied with the proper indication and not as a routine method. The lack of standardized AOA methodologies led to the development and launch of a ready-to-use AOA solution and the number of publications dealing with AOA is constantly increasing. This chapter describes a proven protocol for AOA that has been used in clinical practice for more than two decades.
Information
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- Chapter
- Information
- Principles of IVF Laboratory PracticeLaboratory Set-Up, Training and Daily Operation, pp. 201 - 206Publisher: Cambridge University PressPrint publication year: 2023
References
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