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Chapter 24 - Set-Up and Adapted Protocol for Piezo-ICSI

from Section 4 - Insemination/ICSI

Published online by Cambridge University Press:  07 August 2023

By
Kenichiro Hiraoka and
Kiyotaka Kawai
Edited by
Markus H. M. Montag and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

Piezo-ICSI is one of the intracytoplasmic sperm injection (ICSI) techniques using a piezo-actuator. This technique requires the use of a drive unit, a micropipette with a blunt-ended tip and an operation liquid. Piezo-ICSI can be applied for sperm immobilization, opening of the zona pellucida without oocyte deformation, and to semi-automatically break the oolemma membrane without the need for aspiration of cytoplasm into the injection pipette. The piezo-ICSI process is stable and easy and can contribute to standardizing the ICSI technique. In this chapter, we describe the set-up and protocol of piezo-ICSI for human oocytes.

Keywords

piezo-ICSIICSIfertilizationsperm

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Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 178 - 185
Publisher: Cambridge University Press
Print publication year: 2023

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References

Palermo, G., Joris, H., Devroey, P. and Van Steirteghem, A. C. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 1992; 340 (8810):17–18.CrossRefGoogle ScholarPubMed
Kimura, Y. and Yanagimachi, R. Intracytoplasmic sperm injection in the mouse. Biol Reprod 1995; 52(4):709–20.CrossRefGoogle ScholarPubMed
Huang, T., Kimura, Y. and Yanagimachi, R. The use of piezo micromanipulation for intracytoplasmic sperm injection of human oocytes. J Assist Reprod Genet 1996; 13(4):320–8.CrossRefGoogle ScholarPubMed
Yanagida, K., Katayose, H., Yazawa, H., et al. The usefulness of a piezo-micromanipulator in intracytoplasmic sperm injection in humans. Hum Reprod 1999; 14(2):448–3.CrossRefGoogle ScholarPubMed
Takeuchi, S., Minoura, H., Shibahara, T., et al. Comparison of piezo-assisted micromanipulation with conventional micromanipulation for intracytoplasmic sperm injection into human oocytes. Gyn Obstet Invest 2001; 52(3):158–62.Google ScholarPubMed
Hiraoka, K. and Kitamura, S. Clinical efficiency of Piezo-ICSI using micropipettes with a wall thickness of 0.625 μm. J Assist Reprod Genet 2015; 32(12):1827–33.CrossRefGoogle Scholar
Furuhashi, K., Saeki, Y., Enatsu, N., et al. Piezo-assisted ICSI improves fertilization and blastocyst development rates compared with conventional ICSI in women aged more than 35 years. Reprod Med Biol 2019; 18(4):357–61.CrossRefGoogle Scholar
Fujii, Y., Endo, Y., Mitsuhata, S., Hayashi, M. and Motoyama, H. Evaluation of the effect of piezo-intracytoplasmic sperm injection on the laboratory, clinical, and neonatal outcomes. Reprod Med Biol 2020; 19(2):198–205.Google Scholar
Setti, A. S., Ferreira, R. C., Braga, D., et al. Intracytoplasmic sperm injection outcome versus intracytoplasmic morphologically selected sperm injection outcome: a meta-analysis. Reprod Biomed Online 2010; 21(4):450–5.Google Scholar
Montag, M., Schimming, T. and van der Ven, H. Spindle imaging in human oocytes: the impact of the meiotic cell cycle. Reprod Biomed Online 2006; 12(4):442–6.CrossRefGoogle ScholarPubMed
Menz, D. H., Feltgen, T., Menz, H., et al. How to ward off retinal toxicity of perfluorooctane and other perfluorocarbon liquids? Invest Ophtalmol Vis Sci 2018; 59(12):4841–6.Google ScholarPubMed

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