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A comparison of behavioural methodologies utilised to quantify deviations in piglet behaviour associated with castration

Published online by Cambridge University Press:  01 January 2023

RM Park ,
MC Cramer ,
BK Wagner ,
P Turner ,
LE Moraes ,
AV Viscardi ,
JF Coetzee  and
MD Pairis-Garcia
RM Park
Affiliation:
Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA
MC Cramer
Affiliation:
Department of Animal Sciences, College of Agricultural Sciences, Colorado State University, Fort Collins, CO 80521, USA
BK Wagner
Affiliation:
Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA
P Turner
Affiliation:
Department of Pathobiology, University of Guelph, ON, Canada N1G 2W1l Charles River, Wilmington, MA 01887, USA
LE Moraes
Affiliation:
Department of Animal Sciences, College of Food, Agriculture and Environmental Sciences, The Ohio State University, Columbus, OH 43210, USA
AV Viscardi
Affiliation:
Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA
JF Coetzee
Affiliation:
Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA
MD Pairis-Garcia*
Affiliation:
1060 William Moore Dr, Raleigh, NC 27607, USA Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA
*
* Contact for correspondence: pairis-garcia@ncsu.edu
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Abstract

Surgical castration is a painful procedure that is routinely performed without pain relief on commercial pig (Sus scrofa domesticus) farms. Previous research has focused on quantifying piglet pain response through behaviours. However, to date, behavioural sampling methodologies used to quantify pain associated with castration have not been validated. Therefore, the objective of this study was to validate scan sampling methodologies (2-min, 3-min, 5-min, 10-min and 15-min intervals) to quantify piglet pain responses expressed by castrated piglets’ behaviour. A total of 39 Yorkshire-Landrace × Duroc male piglets (five days of age) were surgically castrated using a scalpel blade. Behaviour frequency and duration (scratching, spasms, stiffness, tail wagging and trembling) of each piglet were continuously collected for the first 15 min of the following hours relative to castration (-24, 1-8 and 24). To determine if the sampling interval accurately reflected true duration and frequency for each behaviour, as determined by continuous observation, criteria previously utilised from other behavioural validation studies were used: coefficient of determination above 0.9, slope not statistically different from one and intercept not statistically different from zero. No scan sampling interval provided accurate estimates for any behavioural indicators of pain. The results of this study suggest that continuous sampling is the most appropriate methodology to fully capture behaviour specific to pain associated with castration. Using validated behavioural methodologies in future research can assist in the development of objective, science-based protocols for managing pig pain.

Keywords

animal welfarebehaviourcastrationpainswinevalidation

Information

Type
Research Article
Information
Animal Welfare , Volume 29 , Issue 3 , August 2020 , pp. 285 - 292
Copyright
© 2020 Universities Federation for Animal Welfare

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References

Altmann, J 1974 Observational study of behaviour: Sampling methods. Behaviour 49: 227–266. https://doi.org/10.1163/156853974X00534CrossRefGoogle ScholarPubMed
American Veterinary Medical Association 2013 Swine castration https://www.avma.org/KB/Policies/Pages/Swine-Castration.aspxGoogle Scholar
Burkemper, MC, Pairis-Garcia, MD, Moraes, LE, Park, RM and Moeller, SJ 2019 Effects of oral meloxicam and topical lido-caine on pain associated behaviours of piglets undergoing surgical castration. Journal of Applied Animal Welfare Science 23(2): 209–218. https://doi.org/10.1080/10888705.2019.1590717CrossRefGoogle ScholarPubMed
Chen, JM, Schütz, KE and Tucker, CB 2016 Technical note: Comparison of instantaneous sampling and continuous obser-vation of dairy cattle behaviour in freestall housing. Journal of Dairy Science 99(10): 8341–8346. https://doi.org/10.3168/jds.2016-11351CrossRefGoogle Scholar
Crews, J, Braude, S, Stephenson, C and Clardy, T 2002 The Ethogram and Animal Behaviour Research. Washington University: St Louis, MO, USAGoogle Scholar
Czerwinski, VH, Smith, BP, Hynd, PI and Hazel, SJ 2017 Sampling maternal care behaviour in domestic dogs: What’s the best approach? Behavioural Processes 140: 41–46. https://doi.org/10.1016/j.beproc.2017.03.018CrossRefGoogle ScholarPubMed
Daigle, CL and Siegford, JM 2014 When continuous observations just won’t do: Developing accurate and efficient sampling strategies for the laying hen. Behavioural Processes 103: 58–66 https://doi.org/10.1016/j.beproc.2013.11.002CrossRefGoogle ScholarPubMed
Davis, K, Seddon, Y, Creutzinger, K, Bouvier, M and Brown, J 2017 An investigation into the use of sucrose to reduce castration pain in piglets. Canadian Journal of Animal Science 97(3): 439–447. https://doi.org/10.1139/CJAS-2016-0170Google Scholar
De Oliveira, FA, Luna, SP, do Amaral, JB, Rodrigues, KA, Sant’Anna, AC, Daolio, M and Brondani, JT 2014 Validation of the UNESP-Botucatu unidimensional composite pain scale for assessing post-operative pain in cattle. BMC Veterinary Research 10(1): 200. https://doi.org/10.1080/10888705.2019.1590717CrossRefGoogle ScholarPubMed
Dzikamunhenga, RS, Anthony, R, Coetzee, J, Gould, S, Johnson, A, Karriker, L, McKean, J, Millman, ST, Niekamp, SR and O’Conner, AM 2014 Pain management in the neonatal piglet during routine management procedures. Part 1: a systematic review of random-ized and non-randomized intervention studies. Animal Health Research Reviews 15(1): 14–38. https://doi.org/10.1017/S1466252314000061CrossRefGoogle Scholar
European Commission 2010 Alternatives to pig castration: European declaration on alternative to surgical castration of pigs. https://ec.europa.eu/food/animals/welfare/practice/farm/pigs/cas-tration_alternatives_enGoogle Scholar
Gottardo, F, Scollo, A, Contiero, B, Ravagnani, A, Tavella, G, Bernardini, D, De Benedictis, GM and Edwards, SA 2016 Pain alleviation during castration of piglets: a comparative study of different farm options. Journal of Animal Science 94: 5077–5088. https://doi.org/10.2527/jas.2016-0843CrossRefGoogle ScholarPubMed
Hansson, M, Lundeheim, N, Nyman, G and Johansson, G 2011 Effect of local anaesthesia and/or analgesia on pain respons-es induced by piglet castration. Acta Veterinaria Scandinavica 53: 34. https://doi.org/10.1186/1751-0147-53-34CrossRefGoogle ScholarPubMed
Hay, M, Vulin, A, Génin, S, Sales, P and Prunier, A 2003 Assessment of pain induced by castration in piglets: Behavioral and physiological responses over the subsequent 5 days. Applied Animal Behavior Science 82: 201–218. https://doi.org/10.3389/fvets.2016.00108CrossRefGoogle Scholar
Hellebrekers, LJ 2000 Animal pain: a practice-orientated approach to an effective pain control in animals. Van Der Wees Utigerverij: Utrecht, The NetherlandsGoogle Scholar
Hepworth, G and Hamilton, A 2001 Scan sampling and water-fowl activity budget studies: design and analysis considerations. Behaviour 138: 1391–1405. https://doi.org/10.1163/156853901317367654CrossRefGoogle Scholar
Hug, PJ, Cap, VH, Honegger, J, Schupbach-Regula, G, Schwarz, A and Bettschart-Wolfensberger, R 2018 Optimization of analgesia for piglet castration under isoflurane anaesthesia with parental butorphanol, meloxicam or intratestic-ular lidocaine. Schweizer Archiv fur Tierheikunde 160(7-8): 461–467. https://doi.org/10.17236/sat00169CrossRefGoogle ScholarPubMed
Keita, A, Pagot, E, Prunier, A and Guidarini, C 2010 Pre-emptive meloxicam for postoperative analgesia in piglets undergoing surgical castration. Veterinary Anaesthesia and Analgesia 37: 367–374. https://doi.org/10.1111/j.1467-2995.2010.00546.xGoogle ScholarPubMed
Kluviers-Poodt, M, Zonderland, JJ, Verbraak, J, Lambooij, E and Hellebrekers, LJ 2013 Pain behaviour after castration of piglets; effect of pain relief with lidocaine and/or meloxicam. Anima 7: 1158–1162. https://doi.org/10.1017/S1751731113000086CrossRefGoogle Scholar
Lehner, PN 1987 Design and execution of animal behavior research: an overview. Journal of Animal Science 65: 1213–1219. https://doi.org/10.2527/jas1987.6551213xCrossRefGoogle ScholarPubMed
Lehner, PN 1992 Sampling methods in behaviour research. Poultry Science 71(4): 643–649. https://doi.org/10.3382/ps.0710643CrossRefGoogle Scholar
Lendvai, AZ, Akcay, C, Ouyang, JQ, Dakin, R, Domalik, AD, St John, PS, Stanback, M, Moore, IT and Bonier, F 2015 Analysis of the optimal duration of behavioral observations based on an automated continuous monitoring system in tree swallows (Tachycineta bicolor): Is one hour good enough? PLoS One 10(11): e0141194. https://doi.org/10.1371/journal.pone.0141194CrossRefGoogle Scholar
Llamas Moya, S, Boyle, LA, Lynch, PB and Arkins, S 2008 Effect of surgical castration on the behavioural and acute phase responses of 5-day-old piglets. Applied Animal Behaviour Science 111(1-2): 133–145. https://doi.org/10.1016/j.applanim.2007.05.019CrossRefGoogle Scholar
Madruga, A, Mainau, E, Gonzalez, LA, Rodriguez-Prado, M, Ruiz de la Torre, JL, Manteca, X and Ferret, A 2017 Technical note: Recording rules for behavioral studies in growing heifers fed high-concentrate diets. Journal of Animal Science 95: 2339–2344. https://doi.org/10.2527/jas2016.1037Google ScholarPubMed
Malachowski, CP and Dugger, BD 2018 Hawaiian duck behav-ioural patterns in seasonal wetlands and cultivated taro. The Journal of Wildlife Management 82(4): 840–849. https://doi.org/10.1002/jwmg.21429Google Scholar
Martin, P and Bateson, P 2012 Measuring Behaviour. Cambridge University Press: Cambridge, UK. https://doi.org/10.1017/CBO9781139168342Google Scholar
Martin, P and Bateson, PPG 1993 Measuring Behaviour: An Introductory Guide, Second Edition. Cambridge University Press: New York, NY, USACrossRefGoogle Scholar
McGlone, J, Guay, K and Garcia, A 2016 Comparison of intra-muscular or subcutaneous injections vs castration in pigs: impacts on behavior and welfare. Animals 6(9): 52. https://doi.org/10.3390/ani6090052CrossRefGoogle ScholarPubMed
Mellor, DJ 2016 Updating animal welfare thinking: Moving beyond the ‘Five Freedoms’ towards ‘a life worth living’. Animals 6(3): 21. https://doi.org/10.3390/ani6030021CrossRefGoogle ScholarPubMed
Mitlohner, FM, Morrow-Tesch, JL, Wilson, SC, Dailey, JW and McGlone, JJ 2001 Behavioral sampling techniques for feed-lot cattle. Journal of Animal Science 79: 1189–1193. https://doi.org/10.2527/2001.7951189xCrossRefGoogle Scholar
National Farm Animal Care Council 2014 Code of Practice for the Care and Handling of Pigs. https://www.nfacc.ca/pdfs/codes /pig_code_of_practice.pdfGoogle Scholar
National Pork Board 2018 US Packing sector. https://www.pork.org/facts/stats/u-s-packing-sector/Google Scholar
National Research Council 2012 Nutrient Requirements of Swine, 11th Edition. National Academies Press: Washington, DC, USA. https://doi.org/10.17226/13298Google Scholar
New Zealand Government 2018 Code of Welfare: Animal Husbandry Procedures. https://www.mpi.govt.nz/dmsdocu-ment/1443/directGoogle Scholar
Nielsen, MLK, Sprogis, KR, Bejder, L, Madsen, PT and Christiansen, F 2019 Behavioural development in southern right whale calves. Marine Ecology Progress Series 629: 219–234. https://doi.org/10.3354/meps13125CrossRefGoogle Scholar
O’Connor, A, Anthony, R, Bergamasco, L, Coetzee, J, Gould, S, Johnson, AK, Karriker, LA, Marchant-Forde, JN, Martineau, GS, McKean, J, Millman, ST, Niekamp, S, Pajor, EA, Rutherford, K, Sprague, M, Sutherland, M, von Borell, E and Dzikamunhenga, RS 2014 Pain management in the neonatal piglet during routine management procedures Part 2: Grading the quality of evidence and the strength of recommendations. Animal Health Research Reviews 15(1): 39–62. https://doi.org/10.1017/S1466252314000073CrossRefGoogle ScholarPubMed
Pig Code of Practice Scientific Committee 2012 Code of Practice for the Care and Handling of Pigs: Review of Scientific Research on Priority Issues. National Farm Animal Care Council: Lacombe, AB, USAGoogle Scholar
Primary Industries Standing Committee 2008 Model Code of Practice for the Welfare of Animals Pigs, Third Edition. http://www.publish.csiro.au/ebook/download/pdf/5698Google Scholar
Pullin, AN, Pairis-Garcia, MD, Campbell, BJ, Campler, MR and Proudfoot, KL 2017a Technical note: Instantaneous sam-pling intervals validated from continuous video observation for behavioral recording of feedlot lambs. Journal of Animal Science 95: 4703–4707. https://doi.org/10.2527/jas2017.1835CrossRefGoogle Scholar
Pullin, AN, Pairis-Garcia, MD, Campler, MR and Proudfoot, KL 2017b Validation of scan sampling techniques for behavioural observations of pastured lambs. Animal Welfare 25: 185–190. https://doi.org/10.7120/09627286.26.2.185CrossRefGoogle Scholar
Rault, JL and Lay, DC Jr 2011 Nitrous oxide by itself is insuf-ficient to relieve pain due to castration in piglets. Journal of Animal Science 89(10): 3318–3325. https://doi.org/10.2527/jas.2011-4104CrossRefGoogle Scholar
Ross, L, Cressman, MD, Cramer, MC and Pairis-Garcia, MD 2019 Validation of alternative behavioral observation methods in young broiler chickens Poultry Science 98: 6225–6231. https://doi.org/10.3382/ps/pez475CrossRefGoogle ScholarPubMed
Roughan, JV and Flecknell, PA 2003 Evaluation of a short duration behaviour-based post-operative pain scoring system in rats. European Journal of Pain 7: 397–406. https://doi.org/10.1016/S1090-3801(02)00140-4CrossRefGoogle Scholar
Studd, EK, Landry-Cuerrier, M, Menzie, AK, Boutin, S, McAdam, AG, Lane, JE and Humpries, MM 2019 Behavioral classification of low-frequency acceleration and temperature data from a free-ranging small mammal Ecology and Evolution 9: 619–630. https://doi.org/10.1002/ece3.4786CrossRefGoogle ScholarPubMed
Sutherland, MA 2015 Welfare implications of invasive piglet husbandry procedures, methods of alleviation and alternatives: a review. New Zealand Veterinary Journal 63: 52–57. https://doi.org/10.1080/00480169.2014.961990Google ScholarPubMed
Sutherland, MA, Backus, BL, Brooks, TA and McGlone, JJ 2017 The effect of needle-free administration of local anesthetic on the behavior and physiology of castrated pigs. Journal of Veterinary Behaviour 21: 71–76. https://doi.org/10.1016/j.jveb.2017.07.003CrossRefGoogle Scholar
Sutherland, MA, Davis, BL, Brooks, TA and Coetzee, JF 2012 The physiological and behavioral response of pigs castrated with and without anesthesia or analgesia. Journal of Animal Science 90(7): 2211–2221. https://doi.org/10.2527/jas.2011-4260Google ScholarPubMed
Sutherland, MA, Davis, BL, Brooks, TA and McGlone, JJ 2010 Physiology and behavior of pigs before and after castration: effects of two topical anesthetics. Animal 4(12): 2071–2079. https://doi.org/10.1017/S1751731110001291CrossRefGoogle ScholarPubMed
Tardin, RH, Pinto, MP, Alves, MAS and Simão, SM 2014 Behavioral event occurrence differs between behavioural states in Sotalia guianensis (Cetarctiodactyla: Delphinidae) dolphins: a multi-variate approach. Zoologia 31(1): 1–7. https://doi.org/10.1590/S1984-46702014000100001CrossRefGoogle Scholar
Telles, FG, Luna, SPL, Teixeira, G and Berto, DA 2016 Long-term weight gain and economic impact in pigs castrated under local anesthesia. Veterinary and Animal Science 1: 36–39. https://doi.org/10.1016/j.vas.2016.11.003Google Scholar
Van Beirendonck, S, Driessen, B, Verbeke, G and Geers, R 2011 Behavior of piglets after castration with or without carbon dioxide anesthesia Journal of Animal Sciences 89: 3310–3317. https://doi.org/10.2527/jas.2010-3104Google ScholarPubMed
Viscardi, AV, Hunniford, M, Lawlis, P, Leach, M and Turner, PV 2017 Development of a piglet grimace scale to evaluate piglet pain using facial expressions following castration and tail docking: a pilot study. Frontiers of Veterinary Science 4: 51. https://doi.org/10.3389/fvets.2017.00051CrossRefGoogle ScholarPubMed
Viscardi, AV and Turner, PV 2018a Efficacy of buprenorphine for management of surgical castration pain in piglets. BMC Veterinary Research 14: 318. https://doi.org/10.1186/s12917-018-1643-5CrossRefGoogle ScholarPubMed
Viscardi, AV and Turner, PV 2018b Use of meloxicam or keto-profen for piglet pain control following surgical castration. Frontiers of Veterinary Science 5: 299. https://doi.org/10.3389/fvets.2018.00299CrossRefGoogle ScholarPubMed
Viscardi, AV and Turner, PV 2019 Use of meloxicam, buprenorphine and Maxilene®to assess a multimodal approach for piglet pain management, part 1: surgical castration. Animal Welfare 28: 487–498. https://doi.org/10.7120/09627286.28.4.487CrossRefGoogle Scholar
Von Borell, E, Baumgartner, J, Giersing, M, Jaggen, N, Prunier, A, Tuyttens, FAM and Edwards, SA 2009 Animal welfare implications of surgical castration and its alternatives in pigs Animal 3(11): 1488–1496. https://doi.org/10.1017/S1751731109004728CrossRefGoogle ScholarPubMed
Weary, DM, Niel, L, Flower, FC and Fraser, D 2006 Identifying and preventing pain in animals. Applied Animal Behavior Science 100(1-2): 64–76. https://doi.org/10.1016/j.applan-im.2006.04.013CrossRefGoogle Scholar
Whalin, L, Pairis-Garcia, M, Proudfoot, K, Stalder, K and Johnson, A 2016 Validating behavioral sampling techniques for lame sows administered flunixin meglumine and meloxicam. Livestock Science 191: 103–107. https://doi.org/10.1016/j.livsci.2016.07.017CrossRefGoogle Scholar
Yeates, J 2016 Quality of life and animal behaviour. Applied Animal Behaviour Science 181: 19–26. https://dx.doi.org/10.1016/j.applan-im.2016.04.018CrossRefGoogle Scholar
Yun, J, Ollila, A, Valros, A, Larenza-Menzies, P, Heinonen, M, Oliviero, C and Peltoniemi, O 2019 Behavioural alterations in piglets after surgical castration: Effects of analgesia and anaes-thesia. Research in Veterinary Science 125: 36–42. https://doi.org/10.1016/j.rvsc.2019.05.009CrossRefGoogle Scholar