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The determination of rhodopsin structure may require alternative approaches

Published online by Cambridge University Press:  04 February 2010

Arlene D. Albert
Affiliation:
Department of Ophthalmology, University at Buffalo School of Medicine (SUNY), Buffalo, NY 14214. bchphll@ubvms.cc.buffalo.eduDepartment of Biochemistry, University at Buffalo School of Medicine (SUNY), Buffalo, NY 14214. bchphll@ubvms.cc.buffalo.edu
Philip L. Yeagle
Affiliation:
Department of Biochemistry, University at Buffalo School of Medicine (SUNY), Buffalo, NY 14214. bchphll@ubvms.cc.buffalo.edu

Abstract

The structure of rhodopsin is a subject of intense interest. Solving the structure by traditional methods has proved exceedingly challenging. It may therefore be useful to confront the problem by a combination of alternate techniques. These include FTIR (Fourier transform infrared spectroscopy) and AFM (atomic force microscopy) on the intact protein. Furthermore, additional insights may be gained through structural investigations of discrete rhodopsin domains.

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