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Physicochemical properties of protein-smectite and protein-Al(OH)x-smectite complexes

Published online by Cambridge University Press:  09 July 2018

A. Violante,
A. de Cristofaro,
M.A. Rao and
L. Gianfreda
A. Violante
Affiliation:
Dipartimento di Scienze Chimico-Agrarie, Università di Napoli, “Federico II”, 80055 Portici, Napoli, Italy
A. de Cristofaro
Affiliation:
Dipartimento di Scienze Chimico-Agrarie, Università di Napoli, “Federico II”, 80055 Portici, Napoli, Italy
M.A. Rao
Affiliation:
Dipartimento di Scienze Chimico-Agrarie, Università di Napoli, “Federico II”, 80055 Portici, Napoli, Italy
L. Gianfreda
Affiliation:
Dipartimento di Scienze Chimico-Agrarie, Università di Napoli, “Federico II”, 80055 Portici, Napoli, Italy
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Abstract

Proteins (catalase, albumin, pepsin and lysozyme with different molecular weights and isoelectric points) were differently adsorbed at pH 7.0 on the clay fraction of three raw Na-saturated smectites (Crook and Uri montmorillonites and one hectorite). The adsorption isotherms of proteins on clay minerals showed typical Langmuir characteristics. Lysozyme was adsorbed under the effect of electrostatic interactions between the opposite charges of clay surfaces and protein molecules, whereas catalase and albumin were adsorbed under the effect of non-electrostatic forces. Pepsin was held in relatively high amounts only on the surfaces of hectorite. Proteins were intercalated in the interlayers spaces of smectites, usually undergoing extensive unfolding. Protein-smectite complexes showed different behaviour to heating treatment. Some complexes remained practically unchanged after heating at 200°C. Presence of ‘wrecks’ of interlayered materials was found after heating at 500°C for two hours. The amounts of proteins adsorbed on the external and interlamellar surfaces of clay minerals, partially coated with OH-Al species, were much lower than those fixed on the clean clays. Only lysozyme was intercalated in chlorite-like complexes.

Information

Type
Research Article
Information
Clay Minerals , Volume 30 , Issue 4 , December 1995, pp. 325 - 336
Copyright
Copyright © The Mineralogical Society of Great Britain and Ireland 1995

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References

Armstrong, D.E. & Chesters, G. (1964) Properties of protein-bentonite complexes as influenced by equilibration conditions. Soil Sci. 98, 39–52.Google Scholar
Barnhisel, R.I. & Bertsch, P.M. (1989) Chlorites and hydroxy interlayered vermiculite and smectite. Pp. 729–778 in: Minerals in Soil Environments (Dixon, J.B. & Weed, S.B., editors), 2nd edn,. Soil Science Society of America, Madison, Wisconsin.Google Scholar
Barroug, A., Lemaitre, J. & Rouxhet, P.G. (1989) Lysozyme on apatites: A model of protein adsorption controlled by electrostatic interactions. Colloids Surf. 37, 339–355.Google Scholar
Borchardt, G. (1989) Smectites. Pp. 675-727 in: Minerals in Soil Environments (Dixon, J.B. & Weed, S.B., editors), 2nd edn. Soil Science Society of America, Madison, Wisconsin.Google Scholar
Buondonno, A., Felleca, D. & Violante, A. (1989) Properties of organo-mineral complexes formed by different addition sequences of hydroxy-Al, montmorillonite, and tannic acid. Clays Clay Miner. 37, 235–242.Google Scholar
Fusi, P., Ristort, G.G., Calamai, L. & Stotzky, G. (1989) Adsorption and binding of protein on ‘clean’ (homoionic) and ‘dirty’ (coated with Fe oxyhydroxides) montmorillonite, illite and kaolinite. Soil Biol. Biochem. 21, 911–920.Google Scholar
Gianfreda, L., Rag, M.A. & Violante, A. (1991) Invertase ([3-fructosidase): Effects of montmorillonite, Al-hydroxide and Al(OH)x-montmorillonite complex on activity and kinetic properties. Soil Biol. Biochem, 23, 581–587.Google Scholar
Gianfreda, L., Rag, M.A. & Violante, A. (1992) Adsorption, activity and kinetic properties of urease on montmorillonite, alluminium hydroxide and Al(OH)x-montmorillonite complexes. Soil Biol. Biochem. 24, 51–58.CrossRefGoogle Scholar
Giles, C.H., Smith, D. & Htiitson, A. (1974) A general treatment and classification of the solute adsorption isotherm. I. Theoretical. J. Colloid Interface Sci. 47, 755–765.Google Scholar
Harter, R.D. (1975) Effect of exchange cations and solution ionic strength on formation and stability of smectite-protein complexes. Soil Sci. 120, 174–181.CrossRefGoogle Scholar
Harter, R.D. & Stotzky, G. (1971) Formation of clayprotein complexes. Soil Sci. Soc. Am. Proc. 35, 383–389.Google Scholar
Harter, R.D. & Stotzky, G. (1973) X-ray diffraction, electron microscopy, electrophoretic mobility, and pH of some stable smectite-protein complexes. Soil Sci. Soc. Am. Proc. 37, 116–123.Google Scholar
Huang, P.M. & Violante, A. (1986) Influence of organic acids on crystallization and surface properties of precipitation products of aluminum. Pp. 159–221 in: Interactions of Soil Minerals with Natural Organics and Microbes (Huang, P.M. & Schnitzer, M., editors), Soil Science Society of America, Spec. Pub. 17, Madison, Wisconsin.CrossRefGoogle Scholar
Larsson, N. & Siffert, B. (1983) Formation of lysozyme-containing crystals of montmorillonite. J. Coll. Interf. Sci. 93, 424–431.CrossRefGoogle Scholar
Mckeague, J.A. (1978) Manual on Soil Sampling and Methods of Analysis. Pp. 212. Canadian Society of Soil Science Ottawa.Google Scholar
Mclarern, A.D., Peterson, G.H. & Barshad, I. (1958) The adsorption and reactions of enzymes and proteins on clay minerals: IV. Kaolinite and montmorillonite. Soil Sci. Soc. Am. Proc. 22, 239–244.Google Scholar
Naidja, A., Violante, A. & Huang, P.M. (1995) Adsorption of tyrosinase on montmorillonite as influenced by hydroxyaluminum coatings. Clays Clay Miner. 43 (in press).Google Scholar
Norde, W. (1986) Adsorption of proteins from solutions at the solid-liquid interface. Adv. Coll. Interf. Sci. 25, 267–340.Google Scholar
Norde, W. & Lyklema, J. (1978) The adsorption of human plasma albumin and bovine pancreas ribonuclease at negatively charged polystyrene surfaces: I. Adsorption isotherms. Effects of charge, ionic strength, and temperature. J. Coll. Interf Sci. 66, 257–265.Google Scholar
Quiquampoix, H. (1987) A stepwise approch to the understanding of extracellular enzyme activity in soil: I. Effect of electrostatic interactions on the conformation of a β-D-glucosidase adsorbed on different mineral surfaces. Biochimie, 69, 753–763.Google Scholar
Quiquampoix, H. & Ratcliffe, R.G. (1992) A 31p NMR study of the adsorption of bovine serum albumin on montmorillonite using phosphate and the paramagnetic cation Mn2+: Modification of conformation with pH. J. Coll. Interf. Sci. 148, 343–352.CrossRefGoogle Scholar
Schnitzer, M. & Kodama, H. (1977) Reactions of minerals with soil humic substances. Pp. 741–770 in: Minerals in Soil Environments (Dixon, J.B. & Weed, S.B., editors). Soil Science Society of America, Madison, Wisconsin.Google Scholar
Theng, B.K.G. (1979) Formation and Properties of Clay-Polymer Complexes. Elsevier, New York.Google Scholar