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A blastogenic test for foot-and-mouth disease

Published online by Cambridge University Press:  15 May 2009

R. C. Wardley,
W. G. Chapman and
A. J. M. Garland
R. C. Wardley
Affiliation:
Animal Virus Research Institute, Pirbright, Surrey
W. G. Chapman
Affiliation:
Animal Virus Research Institute, Pirbright, Surrey
A. J. M. Garland
Affiliation:
Animal Virus Research Institute, Pirbright, Surrey
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A blastogenic test to detect peripheral blood leukocytes specifically sensitized to foot-and-mouth disease virus antigen is described. The test is carried out in microtitre plates and optimum conditions were found by titration. These employed 7.5 × 105 cells/well and 20 complement fixing units of antigen. Peak [3H]thymidine incorporation was found to take place at 2–3 days.

Information

Type
Research Article
Information
Journal of Hygiene , Volume 83 , Issue 3 , December 1979, pp. 507 - 512
Copyright
Copyright © Cambridge University Press 1979

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