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The intestinal flora of colonization-resistant mice

Published online by Cambridge University Press:  15 May 2009

F. Wensinck and
J. G. H. Ruseler-van Embden
F. Wensinck
Affiliation:
Laboratory of Medical Microbiology, Medical Faculty, Rotterdam, The Netherlands
J. G. H. Ruseler-van Embden
Affiliation:
Laboratory of Medical Microbiology, Medical Faculty, Rotterdam, The Netherlands
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To facilitate the isolation of anaerobes from cultures in which swarming organisms were present Roux flasks were adapted to provide anaerobic conditions. Using these flasks, five species of anaerobes were isolated from the caecum of mice resistant to colonization with Escherichia coli and other Gram-negative organisms. These species accounted for the majority of organisms seen in films of caecal material. No aerobes were isolated and the flora of these mice apparently consisted of a limited number of anaerobes representing part of the intestinal flora of conventional mice. Three of the species were clostridia and another was identified as Propionibacterium acnes. One species resembled Catenabacterium but was not identified. One of the Clostridium species could only be isolated when the medium was supplemented with yeast extract and vitamins. This species sporulated on media with ammonium phosphate as the source of nitrogen; tryptone completely suppressed sporulation.

Information

Type
Research Article
Information
Journal of Hygiene , Volume 69 , Issue 3 , September 1971, pp. 413 - 421
Copyright
Copyright © Cambridge University Press 1971

References

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