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The isolation of streptococci from human faeces*

Published online by Cambridge University Press:  15 May 2009

P. F. Unsworth
P. F. Unsworth
Affiliation:
Division of Hospital Infection, Central Public Health Laboratory, Colindale Avenue, London NW9 5HT
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Three selective media were designed for isolation of streptococci from faeces. Samples of faeces from twelve normal adults were suspended and serially diluted in saline or broth saline, and equal volumes of each dilution were spread and incubated on the media. The number of colonies of each different type which developed was counted and one colony of each type was subcultured and identified.

Altogether, streptococci of 13 taxa were found. S. faecium, S. mutans, S. milleri, S. faecalis and S. mitior were each found in over half the samples. Lancefield group B and G streptococci, S. bovis II, an atypical strain of S. bovis I, S. cremoris, S. durans and a dextran-positive strain of S. mitior were each present in 1 or 2 samples. Individual samples contained 2–7 (mean 4·6) streptococcal taxa, and total viable counts of streptococci of 3 × 103−3 × 108 (geometric mean 7 × 105) per g. The significance of these findings is discussed.

Information

Type
Research Article
Information
Journal of Hygiene , Volume 85 , Issue 1 , August 1980, pp. 153 - 164
Copyright
Copyright © Cambridge University Press 1980

References

Ball, L. C. & Parker, M. T. (1979). The cultural and biochemical characters of Streptococcus milleri strains isolated from human sources. Journal of Hygiene 82, 63–78.CrossRefGoogle ScholarPubMed
Carlsson, J. (1967). A medium for isolation of Streptococcus mutans. Archives of Oral biology 12, 1657–8.CrossRefGoogle Scholar
Colman, G. (1970). The classification of streptococcal strains. Ph.D. Thesis, University of London, pp. 31–2.Google Scholar
Colman, G. & Williams, R. E. O. (1972). Taxonomy of some human viridans streptococci. In Streptococci and Streptococcal Diseases; Recognition, Understanding and Management (ed. Wannamaker, L. W. and Matsen, J. M.), pp. 281–99. New York and London: Academic Press.Google Scholar
Cowan, S. T. & Steel, K. J. (1974). Manual for the Identification of Medical Bacteria, 2nd ed. (ed. Cowan, S. T.), Cambridge University Press.Google Scholar
Crowther, J. S. (1971). Transport and storage of faeces for bacteriological examination. Journal of Applied Bacteriology 34, 477–83.CrossRefGoogle ScholarPubMed
Deibel, R. H. & Seeley, H. W. (1974). Family II. Streptococcaceae fam.nov., in Bergey's Manual of Determinative Bacteriology, 8th ed. (ed. Buchanan, R. E. and Gibbons, N. E.), pp. 490–509. Baltimore: The Williams and Wilkins Company.Google Scholar
de Louvois, J., Gortvai, P. & Hurley, R. (1977). Bacteriology of abscesses of the central nervous system: a multicentre prospective study. British Medical Journal ii, 981–4.CrossRefGoogle Scholar
Drasar, B. S. & Hill, M. J. (1974). Human Intestinal Flora, pp. 42–3. London and New York: Academic Press.Google Scholar
Drasar, B. S., Shiner, M. & McLeod, G. M. (1969). Studies on the intestinal flora. I. The bacterial flora of the gastrointestinal tract in healthy and achlorhydric persons. Gastro-enterology 56, 71–9.CrossRefGoogle ScholarPubMed
Elliott, S. D., McCarty, M. & Lancefield, R. (1977). Teichoic acid of group D streptococci with special reference to strains from pig meningitis. Journal of Experimental Medicine 145, 490–99.CrossRefGoogle Scholar
Gorbach, S. L., Nahas, L., Lerner, P. I. & Weinstein, L. (1967). Studies on the intestinal microflora. I. Effects of diet, age and periodic sampling on numbers of faecal micro-organisms in man. Gastroenterology 53, 845–55.CrossRefGoogle Scholar
Hare, R. & Maxted, W. R. (1935). The classification of haemolytic streptococci from the stools of normal pregnant women and of cases of scarlet fever by means of precipitin and biochemical tests. Journal of Pathology and Bacteriology 41, 513–20.CrossRefGoogle Scholar
Islam, A. K. M. S. (1977). Rapid recognition of group B streptococci (letter). Lancet 1, 256–7.CrossRefGoogle Scholar
Kidson, A. (1967). A new selective medium for Streptococcus pyogenes and other streptococci. Journal of Medical Laboratory Technology 24, 179–86.Google ScholarPubMed
Kilian, M., Theilade, E. & Schiøtt, C. R. (1971). The isolation of Streptococcus mutans from human faeces. Archives of Oral biology 16, 553–4.CrossRefGoogle ScholarPubMed
Klein, R. S., Recco, R. A., Catalano, M. T., Edberg, S. C., Casey, J. I. & Steigbigel, N. H. (1977). Association of Streptococcus bovis with adenocarcinoma of the colon. New England Journal of Medicine 297, 800–802.CrossRefGoogle Scholar
Melo, J. C. & Raff, M. J. (1978). Brain abscess due to Streptococcus MG-intermedius (Strepto-coccus milleri). Journal of Clinical Microbiology 7, 529–32.CrossRefGoogle Scholar
Moore, W. E. C. & Holdeman, L. V. (1974). Human faecal flora: the normal flora of 20 Japanese-Hawaiians. Applied Microbiology 27, 961–79.CrossRefGoogle ScholarPubMed
Noble, C. J. (1978). Carriage of group D streptococci in the human bowel. Journal of Clinical Pathology 31, 1182–6.CrossRefGoogle Scholar
Ottens, H. & Winkler, K. C. (1962). Indifferent and haemolytic streptococci possessing group antigen F. Journal of General Microbiology 28, 181–91.CrossRefGoogle ScholarPubMed
Parker, M. T. & Ball, L. C. (1976). Streptococci and aerococci associated with systemic infection in man. Journal of Medical Microbiology 9, 275–302.CrossRefGoogle ScholarPubMed
Parker, M. T. (1977). Neonatal streptococcal infections. Postgraduate Medical Journal 53, 598–606.CrossRefGoogle ScholarPubMed
Pavlova, M. T., Brezenski, F. T. & Litsky, W. (1972). Evaluation of various media for isolation, enumeration and identification of faecal streptococci from natural sources. Health Laboratory Science 9, 289–98.Google ScholarPubMed
Phillips, I., Warren, C., Harrisson, J. M., Sharples, P., Ball, L. C. & Parker, M. T. (1976). Antibiotic susceptibilities of streptococci from the mouth and blood of patients treated with penicillin or lincomycin and clindamycin. Journal of Medical Microbiology 9, 393–404.CrossRefGoogle ScholarPubMed
Poole, P. M. & Wilson, G. (1977). Streptococcus milleri in the appendix. Journal of Clinical Pathology 30, 937–42.CrossRefGoogle ScholarPubMed
Porterfield, J. S. (1950). Classification of the streptococci of subacute bacterial endocarditis. Journal of General Microbiology 4, 92–101.CrossRefGoogle ScholarPubMed
Smith, A. U. (1961). Biological Effects of Freezing and Supercooling, pp. 74–93. London: Edward Arnold.Google Scholar
Waitkins, S. A. (1978). Use of pyruvate fermentation compared with tetrazolium reduction in the differentiation of group D streptococci. Journal of Clinical Pathology 31, 692–5.CrossRefGoogle ScholarPubMed
Waitkins, S. A., Ball, L. C. & Fraser, C. A. M. (1980). A shortened scheme for the identification of indifferent streptococci. Journal of Clinical Pathology 33, 47–52.CrossRefGoogle ScholarPubMed