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A laboratory method to evaluate Pseudocercospora musae's sensitivity to fungicides

Published online by Cambridge University Press:  24 January 2008

Luc de Lapeyre de Bellaire and
Jean-Michel Risède
Luc de Lapeyre de Bellaire
Affiliation:
CIRAD, UPR Systèmes bananes et ananas, CARBAP, BP 832, Douala, Cameroon
Jean-Michel Risède
Affiliation:
CIRAD, UPR Systèmes bananes et ananas, Station de Neufchâteau Sainte-Marie, 97130 Capesterre-Belle-Eau, Guadeloupe jean-michel.risede@cirad.fr
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Abstract

Introduction. This protocol aims at detecting and evaluating sensitivity shifts in Pseudocercospora musae (teleomorph: Mycosphaerella musicola) populations towards fungicides that are currently sprayed to control Sigatoka disease of banana. The principle, key advantages, starting plant material, time required and expected results are presented.Materials and methods. Necessary laboratory materials, and details of the seven steps of the protocol achieved during four days of experiments are described. Results. The protocol results in the observation of conidial germination of P. musae (pattern or germ tube elongation) according to the sensitivity to fungicides.

Keywords

France (Guadeloupe)Musa sp.disease controlmethodslaboratory equipmentpesticide resistancePseudocercospora musaeFrance (Guadeloupe)Musa sp.contrôle de maladiesméthodematériel de laboratoirePseudocercospora musaerésistance aux pesticides

Information

Type
Research Article
Information
Fruits , Volume 63 , Issue 1 , January 2008, pp. 53 - 56
Copyright
© CIRAD, EDP Sciences, 2008

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References

Cronshaw, D.K., Monitoring of Sigatoka disease (Mycosphaerella musicola Leach × Mulder) in the Windward Islands for tolerance to fungicides, Fruits 39 (1984) 163–165.
Smith, C.M., Johnson, L.E.B., Shillingford, C.A.,In vitro methods for monitoring flusilazole sensitivity of Mycosphaerella fijiensis (black Sigatoka of bananas), EPPO Bull. 21 (1991) 332–335. CrossRef
Brun J., La cercosporiose du bananier en Guinée. Étude de la phase ascosporée du Mycosphaerella musicola Leach, Univ. d’Orsay, Thèse, Paris, France, 1963.