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Propagation of citrus somatic hybrids with potential for utilization as rootstocks

Published online by Cambridge University Press:  15 April 2006

Rafael Pio,
Francisco de Assis Alves MourãoFilho,
Beatriz Madalena Januzzi Mendes,
Fábio Albuquerque Entelmann and
André Siqueira Rodrigues Alves
Rafael Pio
Affiliation:
Instituto Agronômico (IAC), Centro Avançado de Pesquisa Tecnológica do Agronegócio de Frutas, Av. Luiz Pereira dos Santos, nº 1500, Corrupira, 13214-820, Jundiaí, SP, Brazil
Francisco de Assis Alves MourãoFilho
Affiliation:
Departamento de Produção Vegetal, USP/ESALQ, CP 9, Piracicaba, SP, 13418-900, Brazil
Beatriz Madalena Januzzi Mendes
Affiliation:
Laboratório de Biotecnologia Vegetal, USP/CENA, CP 96, Piracicaba, SP, 13400-970, Brazil
Fábio Albuquerque Entelmann
Affiliation:
Departamento de Produção Vegetal, USP/ESALQ, CP 9, Piracicaba, SP, 13418-900, Brazil
André Siqueira Rodrigues Alves
Affiliation:
Departamento de Produção Vegetal, USP/ESALQ, CP 9, Piracicaba, SP, 13418-900, Brazil
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Abstract

Introduction. Alternative propagation techniques must be developed in order to reduce the time required to evaluate germplasm in citrus breeding programs. Our study investigated the rooting capacity and initial plant development of different citrus somatic hybrids that have potential for use as rootstocks. Materials and methods. Eleven different hybrids were tested. Cuttings of these plants were placed in trays inside an intermittent mist chamber. After 100 days, the percentage of cuttings with callous and with roots, average number of roots, and root length were recorded. The cuttings were then transferred to plastic bags and kept in the greenhouse. The flush length was recorded monthly. After 7 months, the percentage of plants suitable for grafting and the average scion and root dry mass were calculated. Results and discussion. [Caipira sweet orange + Cleopatra mandarin], [Caipira sweet orange + Volkamer lemon], [Rangpur lime + Sunki mandarin] and [Rohde Red Valencia sweet orange + Volkamer lemon] hybrids developed many roots, while [Caipira sweet orange + Volkamer lemon] and [Rohde Red Valencia sweet orange + Volkamer lemon] hybrids showed the best development after the transplant.

Keywords

BrazilCitrus sp.protoplast fusioncuttingsrootingBrésilCitrus sp.fusion de protoplastesboutureenracinement

Information

Type
Research Article
Information
Fruits , Volume 61 , Issue 1 , January 2006, pp. 1 - 7
Copyright
© CIRAD, EDP Sciences, 2006

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