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Calcium chloride effects on nuclear development in Tetrahymena

Published online by Cambridge University Press:  14 April 2009

D. L. Nanney
D. L. Nanney
Affiliation:
Department of Genetics and Development, University of Illinois, Urbana 61801, U.S.A.
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Conjugating pairs of syngen 1 exposed to 0·1 M-CaCl2 during the time of macronuclear development often abort the new macronuclei and retain the old macronucleus and its associated phenotypes. The new micro-nucleus is retained, however, so that macro–micro heterocaryons can be constructed. Because the macronucleus is from a sexually mature strain, the heterocaryon is capable of mating immediately, without passing a period of sexual immaturity. Macronuclear abortion may prove a useful short-cut in genetic analysis.

Information

Type
Research Article
Information
Genetics Research , Volume 27 , Issue 2 , April 1976, pp. 297 - 302
Copyright
Copyright © Cambridge University Press 1976

References

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