Hostname: page-component-7b7567c8c-tnzcf Total loading time: 0 Render date: 2026-11-15T05:22:49.166Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of unbalanced growth on recombinant formation in E. coli

Published online by Cambridge University Press:  14 April 2009

J. D. Gross
J. D. Gross
Affiliation:
Medical Research Council Microbial Genetics Research Unit, Hammersmith Hospital, London, W. 12
Rights & Permissions [Opens in a new window]

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the 'Save PDF' action button.

When zygotes formed by conjugation between Hfr and F- cells of E. coli are transferred from an amino-acid rich medium to one lacking amino-acids the probability of genetic recombination is reduced. This result is interpreted as indicating that recombination can only occur during a limited period after zygote formation and that the metabolic imbalance caused by the change of medium leads to temporary inhibition of recombination. By an hour after zygote formation the yield of recombinants is no longer affected by transfer to amino-acid free medium.

Information

Type
Research Article
Information
Genetics Research , Volume 4 , Issue 3 , November 1963, pp. 457 - 462
Copyright
Copyright © Cambridge University Press 1963

References

REFERENCES

Adams, M. H. (1959). Bacteriophages. New York: Interscience Publishers Inc.Google Scholar
de Haan, P. G. & Gross, J. D. (1962). Transfer delay and chromosome withdrawal during conjugation in Escherichia coli. Genet. Res. 3, 251–272.Google Scholar
Jacob, F. & Wollman, E. L. (1961). Sexuality and the Genetics of Bacteria. New York: Academic Press.Google Scholar
Kjeldgaard, N. O., Maaløe, O. & Schaechter, M. (1958). The transition between different physiological states during balanced growth of Salmonella typhimurium. J. gen. Microbiol. 19, 607–616.Google Scholar
Lederberg, J. (1957). Sibling recombinants in zygote pedigrees of Escherichia coli. Proc. nat. Acad. Sci., Wash., 43, 1060–1065.Google Scholar
Riley, M. & Pardee, A. B. (1962). Nutritional effects on frequencies of bacterial recombination. J. Bact. 83, 1332–1335.Google Scholar
Tomizawa, J. (1960). Genetic structure of recombinant chromosomes formed after mating in Escherichia coli K12. Proc. nat. Acad. Sci., Wash., 46, 91–101.Google Scholar
Witkin, E. M. (1956). Time, temperature and protein synthesis: a study of ultraviolet-induced mutations in bacteria. Cold Spr. Harb. Symp. quant. Biol. 21, 123–140.Google Scholar
Wollman, E. L. & Jacob, F. (1958). Sur les processus de conjugaison et recombinaison chez Escherichia coli. V. Le mécanisme du transfert de matériel génétique. Ann. Inst. Pasteur 95, 641–666.Google Scholar