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A study on the mate-killer toxin by microinjection in Paramecium

Published online by Cambridge University Press:  14 April 2009

Sadaaki Koizumi and
Sumiko Kobayashi
Sadaaki Koizumi
Affiliation:
Research Institute for Science Education, Miyagi College of Education, Sendai, Japan
Sumiko Kobayashi
Affiliation:
Research Institute for Science Education, Miyagi College of Education, Sendai, Japan
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The killing action by mu toxin, which is contained in the cytoplasm of stock 540, Paramecium primaurelia, was demonstrated against the various stocks of paramecia by means of microinjection. Most of the toxin is present in the soluble fraction of the host cytoplasm. The toxin was precipitated by ammonium sulphate at 50–80% saturation, and was almost completely inactivated by incubation at 60 °C for 30 min. Pre-autogamous paramecia were more sensitive than post-autogamous ones to the toxin. Paramecia which bear endosymbionts were generally resistant to the mate-killer toxin.

Information

Type
Research Article
Information
Genetics Research , Volume 27 , Issue 2 , April 1976, pp. 179 - 185
Copyright
Copyright © Cambridge University Press 1976

References

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