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Survey of genomic repeat sequence-PCRs that detect differences between inbred mouse strains

Published online by Cambridge University Press:  14 April 2009

Philip A. Wood and
Doug A. Hamm
Philip A. Wood*
Affiliation:
Department of Comparative Medicine, Schools of Medicine and Dentistry, University of Alabama at Birmingham, Birmingham, Alabama, USA
Doug A. Hamm
Affiliation:
Department of Comparative Medicine, Schools of Medicine and Dentistry, University of Alabama at Birmingham, Birmingham, Alabama, USA
*
* Corresponding authors.
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Summary

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We have developed molecular markers that distinguish between several inbred and congenic mouse strains using polymerase chain reaction (PCR) amplification of genomic DNA repeat sequences. Mouse genomic DNA, digested with four base recognition site-restriction endonucleases, was amplified by PCR using primers for the following repeat sequences: Bl (Alu homolog), LINE, LLR3, IAP, human Alu and myoglobin. Amplification products analysed by agarose gel electrophoresis and stained with ethidium bromide produced unique DNA fragments, some of which are specific for each of 12 strains tested. This method can be used for molecular analysis of the mouse genome, including genetic monitoring.

Information

Type
Short Paper
Information
Genetics Research , Volume 65 , Issue 2 , April 1995, pp. 151 - 155
Copyright
Copyright © Cambridge University Press 1995

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