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The Bordet-Gengou test applied to the diagnosis of cases of suspected hydatid infection has been used by many workers since first introduced by Ghedini in 1906, using as antigen hydatid fluid from human cysts.
In February, 1924, Dr. Walton recorded Anoplocephala perfoliata, Ascaris equi, Oxyuris equi (curvula), and Cylicostomum tetracanthum from horses in North Wales; and in January 1925, Mr. Bissett, M.R.C.V.S., found, in a horse from Duffryn Mountains (South Wales), a large number of parasitic worms, including Anoplocephala mammillana, Cylicostomum tetracanthum, Strongylus edentatus, S. vulgaris, S. equinus, and one specimen of Oxyuris. These, it seems, are the only recent records concerning helminthic parasites of horses in Wales. It was therefore thought desirable to examine a few horses very carefully.
Earlier in this volume (pp. 27–30), the writer has given an account of an eelworm obtained from a diseased potato tuber, possessing a mouth stylet with six basal swellings, an œsophagus without a muscular bulb such as occurs in Tylenchus and Aphelenchus, a vulva much more posterior in position than in the two genera just mentioned and a uterus without a post-vulvar diverticulum. On these characters the worms were placed in a new genus, Hexatylus.
Adult gravid females were collected from the large intestine of sheep, carefully washed and teased up in water. The grosser material was strained off and the eggs, together with the smaller particles, were cultured in excavated glass cells at 27° C.
In an earlier paper (Goodey, 1924), the writer has given an account of the morphology of the adults of Œsophagostomum dentatum, the development and structure of the free-living larvæ and the biology of the ensheathed infective stage. So far, however, the parasitic larval stages have not been described. In the present paper some of the gaps in our knowledge of the complete life-history of the worm represented by these stages are filled in, not as completely as could be desired owing to the absence from the material of specimens showing the 3rd and 4th ecdyses. The worms found, however, show that the 4th stage larva has a provisional buccal capsule essentially similar to that found in 4th stage larvæ of O. radiatum (Marotel, 1908) and O. columbianum (Veglia, 1924), and in this respect is in line with certain other 4th stage larvæ of the Strongyloidea such as Ancylosloma duodenale (Looss, 1897), and Triodontophorus tenuicollis (Ortlepp, 1925), in which provisional buccal capsules built on the same general plan have been observed.
Two species belonging to the Nematode genus Physaloptera have up to the present been reported from Man; P. caucasica v. Linstow, 1902, which has been obtained on one occasion only from the Caucasus, and P. mordens Leiper, 1908, which has been reported on several occasions from Man and Monkeys from Central Africa. Until quite recently the only account of the former species was that of v. Linstow, and from this it was quite plain that the two species were distinct. Schulz (1926), however, has had the opportunity of re-examining the types of P. caucasica and on the basis of his excellent descriptions and figures it is possible to make a closer comparison between it and P. mordens. Schulz was able to rectify several inaccuracies in v. Linstow's description, and he came to the conclusion that the two species were very closely related, the only difference between the two being that in P. caucasica there is in addition to the three large teeth on each lip also a series of small teeth between and exterior to them on the inner face of the lip, which teeth are absent in P. mordens; also that the former species was smaller than the latter, 14–24 mm. to 29–34 mm. for the male and 24 mm. to 41–100 mm. for the female respectively, and that the eggs of the former were larger.
In “An account of some helminthes contained in Dr. C. M. Wenyon's collection from the Sudan,” published in the Third Report of the Wellcome Research Laboratories at Khartoum, I drew attention in 1908 to the importance of the mouth armature of the Bursate Nematodes and suggested the regrouping of the various genera into three separate families: Strongylidæ, Metastrongylidæ and Eustrongylidæ. The Strongylidæ were divided into three and the Metastrongylidæ into two sub-families. Under the sub-families were listed the names of those genera which I then thought fell into each group, but to none did I append the name of the author or the date of the genus. The genus Protostrongylus occurred in the list of genera of Metastrongylinæ. Where, as in Trichostrongylinæ and Metastrongylinæ new names were suggested, these were indicated by the insertion of N.N. after each. Yet in two important compilations dealing with the classification of the Nematodes published during the present year the authors place on me the responsibility for proposing the generic name “Protostrongylus.” Thus in Baylis and Daubney, page 253, the name is listed Protostrongylus Leiper, 1908, and it is stated that “this name is mentioned by Leiper among genera attributed to his sub-family Metastrongylinæ. No species is mentioned and the name appears to be a nomen nudum.” Yorke and Maplestone similarly listed the genus Protostrongylus Leiper, 1908, among those genera insufficiently known and of uncertain systematic position, stating that it is one “belonging to the sub-family Metastrongy linæ.