Hostname: page-component-7b7567c8c-fvjsw Total loading time: 0 Render date: 2026-11-14T18:24:02.201Z Has data issue: false hasContentIssue false
  • English
  • Français

Hollow Microcapsules Through Layer-by-Layer Self-Assembly of Chitosan/Alginate on E. coli

Published online by Cambridge University Press:  22 May 2020

Michael Y. Yitayew and
Maryam Tabrizian
Michael Y. Yitayew
Affiliation:
Department of Biomedical Engineering, McGill University, 3775 Rue University, Montreal, QC H3A 2B4 Canada
Maryam Tabrizian
Affiliation:
Department of Biomedical Engineering, McGill University, 3775 Rue University, Montreal, QC H3A 2B4 CanadaFaculty of Dentistry, McGill University, 2001 Avenue McGill College, Montreal, QC H3A 1G1 Canada
Get access

Abstract

Hollow microcapsules prepared via layer-by-layer (LbL) self-assembled polyelectrolytes are prevalent biomaterials in the synthesis of biocompatible delivery systems for drugs, imaging probes, and other macromolecules to control biodistribution and lower toxicity in vivo. The use of LbL self-assembly for the synthesis of these capsules provides several benefits including ease of fabrication, abundance in choice of substrates and coating material, as well as application-specific tunability. This study explores the development of hollow microcapsules by LbL assembly of chitosan and alginate onto live E. coli cells, and also provides a proof-of-concept of this capsule as a delivery platform through the encapsulation of quantum dots as a cargo. The study found that robust bilayers of chitosan/alginate can be formed onto the core substrate (E. coli) containing quantum dots as demonstrated with zeta potential analysis. Confocal microscopy was used to verify cell viability and the internalization of quantum dots into the cells as well as confirmation of the coating using fluorescein-labelled chitosan. Furthermore, transmission electron microscopy (TEM) was used to analyse cells coated with four-bilayers and showed a uniform coating morphology with a capsule thickness of 10-20 nm, which increased to 20-50 nm for hollow capsules after cell lysis. Quantum dot retention in the capsules was demonstrated using fluorescence measurements. Overall, the study shows promising results of a novel fabrication method for hollow microcapsules that uses biocompatible polymers and mild core dissolution conditions using cell templates with applications in sustained release of therapeutics and imaging probes.

Information

Type
Articles
Information
MRS Advances , Volume 5 , Issue 46-47: Soft Materials and Biomaterials , 2020, pp. 2401 - 2407
Check for updates
Copyright
Copyright © Materials Research Society 2020

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

Cui, J., Van Koeverden, M.P., Müllner, M., Kempe, K., and Caruso, F., Adv. Colloid Interface Sci. 207, 14 (2014).CrossRefGoogle Scholar
Neu, B., Voigt, A., Mitlöhner, R., Leporatti, S., Gao, C.Y., Donath, E., Kiesewetter, H., Möhwald, H., Meiselman, H.J., and Bäumler, H., J. Microencapsul. 18, 385 (2001).Google Scholar
Ribeiro, C., Borges, J., Costa, A.M.S., Gaspar, V.M., de Zea Bermudez, V., and Mano, J.F., Molecules 23, 10 (2018).Google Scholar
Xie, Y.L., Wang, M.J., and Yao, S.J., Langmuir 25, 8999 (2009).CrossRefGoogle Scholar
Kozlovskaya, V., Zavgorodnya, O., Chen, Y., Ellis, K., Tse, H.M., Cui, W., Thompson, J.A., and Kharlampieva, E., Adv. Funct. Mater. 22, 3389 (2012).CrossRefGoogle Scholar
Ye, S., Wang, C., Liu, X., and Tong, Z., J. Biomater. Sci., Polym. Ed. 16, 909 (2005).CrossRefGoogle Scholar
Elizarova, I.S. and Luckham, P.F., J. Colloid Interface Sci. 470, 92 (2016).CrossRefGoogle Scholar
Lederer, F.L., Günther, T.J., Weinert, U., Raff, J., and Pollmann, K., Microb. Cell Fact. 11, 163 (2012).CrossRefGoogle Scholar
Mamat, U., Wilke, K., Bramhill, D., Schromm, A.B., Lindner, B., Kohl, T.A., Corchero, J.L., Villaverde, A., Schaffer, L., Head, S.R., Souvignier, C., Meredith, T.C., and Woodard, R.W., Microb. Cell Fact. 14, (2015).Google Scholar
Richardson, J.J., Björnmalm, M., and Caruso, F., Science. 348 (6233), 411 (2015).CrossRefGoogle Scholar
Liu, P., Adv. Colloid Interface Sci. 207, 178 (2014).CrossRefGoogle Scholar
Sato, K., Yoshida, K., Takahashi, S., and ichi Anzai, J., Adv. Drug Deliv. Rev. 63, 809 (2011).CrossRefGoogle Scholar
Björnmalm, M., Cui, J., Bertleff-Zieschang, N., Song, D., Faria, M., Rahim, M.A., and Caruso, F., Chem. Mater. 29, 289 (2017).CrossRefGoogle Scholar
Chai, F., Sun, L., He, X., Li, J., Liu, Y., Xiong, F., Ge, L., Webster, T.J., and Zheng, C., Int. J. Nanomedicine 12, 1791 (2017).CrossRefGoogle Scholar
Mansouri, S., Merhi, Y., Winnik, F.M., and Tabrizian, M., Biomacromolecules 12, 585 (2011).CrossRefGoogle Scholar
Hillberg, A.L. and Tabrizian, M., Biomacromolecules 7, 2742 (2006).CrossRefGoogle Scholar
Ma, O., Lavertu, M., Sun, J., Nguyen, S., Buschmann, M.D., Winnik, F.M., and Hoemann, C.D., Carbohydr. Polym. 72, 616 (2008).CrossRefGoogle Scholar
Mansouri, S., Winnik, F.M., and Tabrizian, M., Expert Opin. Drug Deliv. 6, 585 (2009).CrossRefGoogle Scholar
Nayef, L., Castiello, R., and Tabrizian, M., Macromol. Biosci. 17, 1600535 (2017).CrossRefGoogle Scholar