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Human Stem Cell Derived Osteocytes in Bone-on-Chip

Published online by Cambridge University Press:  07 March 2018

E. Budyn ,
N. Gaci ,
S. Sanders ,
M. Bensidhoum ,
E. Schmidt ,
B. Cinquin ,
P. Tauc  and
H. Petite
E. Budyn*
Affiliation:
LMT Laboratory CNRS UMR8535, ENS Cachan, Universite Paris-Saclay, 61 Avenue du Pres. Wilson, 94235 Cachan cedex, France Department of Mechanical Engineering, University of Illinois at Chicago, 842 W. Taylor St., Chicago, IL 60607, U.S.A.
N. Gaci
Affiliation:
LMT Laboratory CNRS UMR8535, ENS Cachan, Universite Paris-Saclay, 61 Avenue du Pres. Wilson, 94235 Cachan cedex, France
S. Sanders
Affiliation:
LMT Laboratory CNRS UMR8535, ENS Cachan, Universite Paris-Saclay, 61 Avenue du Pres. Wilson, 94235 Cachan cedex, France
M. Bensidhoum
Affiliation:
B2OA Laboratory CNRS UMR7052, Universite Paris Diderot, 10 Avenue de Verdun, 75010 Paris, France
E. Schmidt
Affiliation:
Department of Mechanical Engineering, University of Illinois at Chicago, 842 W. Taylor St., Chicago, IL 60607, U.S.A.
B. Cinquin
Affiliation:
LBPA Laboratory CNRS UMR8113, ENS Cachan, Universite Paris-Saclay, 61 Avenue du Pres. Wilson, 94235 Cachan cedex, France
P. Tauc
Affiliation:
LBPA Laboratory CNRS UMR8113, ENS Cachan, Universite Paris-Saclay, 61 Avenue du Pres. Wilson, 94235 Cachan cedex, France
H. Petite
Affiliation:
B2OA Laboratory CNRS UMR7052, Universite Paris Diderot, 10 Avenue de Verdun, 75010 Paris, France
*
*(Email: ebudyn@uic.edu)

Abstract

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Human mesenchymal stem cells were reseeded in decellularized human bone subject to a controlled mechanical loading to create a bone-on-chip that was cultured for over 26 months. The cell morphology and their secretome were characterized using immunohistochemistry and in situ immunofluorescence under confocal microscopy. The presence of stem cell derived osteocytes was confirmed at 547 days. Different cell populations were identified. Some cells were connected by long processes and formed a network. Comparison of the MSCs in vitro reorganization and calcium response to in situ mechanical stimulation were compared to MLOY4 cells reseeded on human bone. The bone-on-chip produced an ECM of which the strength was nearly a quarter of native bone after 109 days and that contained calcium minerals at 39 days and type I collagen at 256 days. The cytoplasmic calcium concentration variations seemed to adapt to the expected in vivo mechanical load at the successive stages of cell differentiation in agreement with studies using fluid shear flow stimulation. Some degree of bone-like formation over a long period of time with the formation of a newly formed matrix was observed.

Keywords

biomaterialbiologicalstress/strain relationship

Information

Type
Articles
Information
MRS Advances , Volume 3 , Issue 26: Biomaterials and Soft Materials , 2018 , pp. 1443 - 1455
Copyright
Copyright © Materials Research Society 2018 

References

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