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Understanding the Enhanced Kinetics of Enzyme-Quantum Dot Constructs

Published online by Cambridge University Press:  28 December 2015

Joyce Breger,
Scott Walper,
Mario Ancona,
Michael Stewart,
Eunkeu Oh,
Kimihiro Susumu and
Igor Medintz
Joyce Breger
Affiliation:
Center for Bio/Molecular Science and Engineering, Code 6900, U.S. Naval Research Laboratory, Washington, DC 20375American Society for Engineering Education, 1818 N Street NW, Suite 600 Washington, DC 20036
Scott Walper
Affiliation:
Center for Bio/Molecular Science and Engineering, Code 6900, U.S. Naval Research Laboratory, Washington, DC 20375
Mario Ancona
Affiliation:
Electronic Science and Technology Division, Code 6800, U.S. Naval Research Laboratory, Washington, DC 20375
Michael Stewart
Affiliation:
Optical Sciences Division, Code 5600, U.S. Naval Research Laboratory, Washington, DC 20375
Eunkeu Oh
Affiliation:
Optical Sciences Division, Code 5600, U.S. Naval Research Laboratory, Washington, DC 20375
Kimihiro Susumu
Affiliation:
Optical Sciences Division, Code 5600, U.S. Naval Research Laboratory, Washington, DC 20375
Igor Medintz*
Affiliation:
Center for Bio/Molecular Science and Engineering, Code 6900, U.S. Naval Research Laboratory, Washington, DC 20375
*
*(Email: igor.medintz@nrl.navy.mil)
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Abstract

Bio-inspired, hybrid architectures employing quantum dots (QDs) appended with functionally active biomolecules such as enzymes have the potential to be utilized in numerous applications. Some examples include nanosensors for medical diagnostics, chemical/biological threat detection, as well as “bio-factories” in complex industrial synthetic processes. The main advantage in creating these nanofactories is increased rates in catalysis and efficiency when enzymes are associated with nanoscaffolds, as shown in numerous studies. However, the mechanism for this enhancement remains elusive. Gaining a fundamental, mechanistic understanding of enzyme-QD nanostructures is important in the development of numerous device applications. In this work, we review an array of enzymes attached to QDs and generate a hypothesis in regards to the unique architecture of the enzyme-nanoparticle (NP) construct that leads to increases in catalysis. We highlight work with phosphotiresterase (PTE) attached to two distinctly sized QDs in neutralizing a simulant nerve agent, as well as in other enzyme systems.

Keywords

kineticsnanoscalecore/shell

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Type
Articles
Information
MRS Advances , Volume 1 , Issue 57: Biomaterials and Soft Materials , 2016, pp. 3831 - 3836
Copyright
Copyright © Materials Research Society 2015 

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References

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