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Bio-Hybrid Materials for Immunoassay-Based Sensing

Published online by Cambridge University Press:  01 February 2011

Jonathan Fang,
Esther H. Lan,
Jing C. Zhou and
Bruce Dunn
Jonathan Fang
Affiliation:
jfang33@yahoo.com University of California, Los Angeles Department of Biomedical Engineering IDP Los Angeles CA 90095 United States
Esther H. Lan
Affiliation:
elan@ucla.edu, University of California, Los Angeles, Department of Materials Science and Engineering, Los Angeles, CA, 90095, United States
Jing C. Zhou
Affiliation:
jingczhou@yahoo.com, University of California, Los Angeles, Department of Biomedical Engineering IDP, Los Angeles, CA, 90095, United States
Bruce Dunn
Affiliation:
bdunn@ucla.edu, University of California, Los Angeles, Department of Materials Science and Engineering, Los Angeles, CA, 90095, United States
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Abstract

The process of encapsulating antibodies in sol-gel was used for sensing various hormones, specifically cortisol, insulin, and C-peptide. A sol-gel optical biosensor for cortisol has been developed for monitoring of crew health on-orbit during space missions. Our studies involving silica sol-gel materials with competitive immunoassays demonstrated linear calibration for cortisol in the range of 2-60 μg/dL, which covers the physiological range of cortisol blood concentration for an adult (2-28 μg/dL). The method of standard additions was used to analyze human serum samples sent to us from a NASA laboratory. Our sol-gel immunosensor values were typically within 20% of the values obtained by NASA-JSC using traditional immuno-binding techniques, with some values having less than a 5% error. Initial results are presented for sensing the hormones insulin and C-peptide.

Keywords

sol-gelbiomedicalsensor

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