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Grating Coupled Surface Plasmon Resonance Enhanced Fluorescence and Its Application for Cell Observation

Published online by Cambridge University Press:  31 January 2011

Xiaoqiang Cui,
Keiko Tawa and
Junji Nishii
Xiaoqiang Cui
Affiliation:
xiaoqiang-cui@aist.go.jp, National Institute of Advanced Industrial Science and Technology (AIST), Research Institute for Cell Engineering, Osaka, Japan
Keiko Tawa
Affiliation:
tawa-keiko@aist.go.jp, National Institute of Advanced Industrial Science and Technology (AIST), Research Institute for Cell Engineering, Osaka, Japan
Junji Nishii
Affiliation:
junji.nishii@aist.go.jp, National Institute of Advanced Industrial Science and Technology (AIST), Research Institute for Cell Engineering, Osaka, Japan
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Abstract

One-dimensional grating with 400 nm pitch was fabricated on a SiO2 glass surface. The grating was applied for detection of fluorescence excited by the electric field of the grating-coupled surface plasmon resonance and highly sensitive observation of fluorescence image by optical microscope. 40-times of fluorescence enhancement was obtained under optimal condition after our systematically investigating depth and duty ratio dependent behavior.

Keywords

nanostructurebiomedicalphotochemical

Information

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1208: Symposium O – , 2009, 1208-O18-12
Copyright
Copyright © Materials Research Society 2010

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