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Interfacing Conducting Polymers and Biological Macromolecules: A Case Study of Insecticide Biosensor Development

Published online by Cambridge University Press:  15 February 2011

Kenneth Marx,
Madhu Ayyagari,
Sanjay Kamtekar,
Rajiv Pande,
Jeong Ok Lim,
Manjunath Kamath,
K. G. Chittibabu,
Sukant Tripathy,
Jayant Kumar and
Lynne Samuelson
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Kenneth Marx
Affiliation:
Center for Advanced Materials and Departments of Chemistry and Physics, University of Massachusetts Lowell, Lowell, MA. 01854
Madhu Ayyagari
Affiliation:
Center for Advanced Materials and Departments of Chemistry and Physics, University of Massachusetts Lowell, Lowell, MA. 01854
Sanjay Kamtekar
Affiliation:
Center for Advanced Materials and Departments of Chemistry and Physics, University of Massachusetts Lowell, Lowell, MA. 01854
Rajiv Pande
Affiliation:
Center for Advanced Materials and Departments of Chemistry and Physics, University of Massachusetts Lowell, Lowell, MA. 01854
Jeong Ok Lim
Affiliation:
Center for Advanced Materials and Departments of Chemistry and Physics, University of Massachusetts Lowell, Lowell, MA. 01854
Manjunath Kamath
Affiliation:
Center for Advanced Materials and Departments of Chemistry and Physics, University of Massachusetts Lowell, Lowell, MA. 01854
K. G. Chittibabu
Affiliation:
Center for Advanced Materials and Departments of Chemistry and Physics, University of Massachusetts Lowell, Lowell, MA. 01854
Sukant Tripathy
Affiliation:
Center for Advanced Materials and Departments of Chemistry and Physics, University of Massachusetts Lowell, Lowell, MA. 01854
Jayant Kumar
Affiliation:
Center for Advanced Materials and Departments of Chemistry and Physics, University of Massachusetts Lowell, Lowell, MA. 01854
Lynne Samuelson
Affiliation:
U.S. Army Natick RD&E Center, Biotechnology Division, Natick, MA. 01760
Joseph Akkara
Affiliation:
U.S. Army Natick RD&E Center, Biotechnology Division, Natick, MA. 01760
David Kaplan
Affiliation:
U.S. Army Natick RD&E Center, Biotechnology Division, Natick, MA. 01760
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Abstract

The creation of smart materials is a current goal of many laboratories including our own. We are involved in studying a number of thin film and monolayer systems which share the common features of involving highly conjugated conducting organic polymers and the evolved properties of specific biological macromolecules. In this report we describe a generic ‘cassette’ methodology for immobilizing biotinylated biological macromolecules to hydrophobic surfaces using a novel class of conducting copolymers of polythiophene. These copolymers are derivatized with long alkyl chains and biotin moieties to bind, respectively, to the hydrophobic surface and the biotinylated species, through the biotin - streptavidin interaction. We utilize the monolayer ‘cassette’ approach to attach a signal transducing biomolecule alkaline phosphatase to the surface of a glass capillary. This produces a flexible system in which chemiluminescence provides the basis for a useful measurement strategy. A novel technique involving the generation of chemiluminescence signal from alkaline phosphatase catalyzed dephosphorylation of a macrocyclic phosphate compound is described. Detection of paraoxon and methyl parathion, both enzyme inhibitors, has been achieved at ppb levels. The technique is rapid, sensitive, and is applicable to the detection of all organophosphorus based insecticides. The technique will be used in developing a fiber;optic biosensor for remote detection of insecticides.

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Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 330: Symposium S – Biomolecular Materials by Design , 1993, 309
Copyright
Copyright © Materials Research Society 1994

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References

REFERENCES

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