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Low-cost and Chemical Resistant Microfluidic Devices Based on Thermoplastic Elastomers for a Novel Biosensor System

Published online by Cambridge University Press:  01 February 2011

I. Stoyanov,
M. Tewes,
S. Glass,
M. Koch and
M. Löhndorf
I. Stoyanov
Affiliation:
Center of advanced european studies and research (caesar), Ludwig-Erhard-Allee 2, D-53175 Bonn, Germany
M. Tewes
Affiliation:
Center of advanced european studies and research (caesar), Ludwig-Erhard-Allee 2, D-53175 Bonn, Germany
S. Glass
Affiliation:
Center of advanced european studies and research (caesar), Ludwig-Erhard-Allee 2, D-53175 Bonn, Germany
M. Koch
Affiliation:
Center of advanced european studies and research (caesar), Ludwig-Erhard-Allee 2, D-53175 Bonn, Germany
M. Löhndorf
Affiliation:
Center of advanced european studies and research (caesar), Ludwig-Erhard-Allee 2, D-53175 Bonn, Germany
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Abstract

Low-cost and chemical resistant microfluidic devices based on thermoplastic elastomers have been fabricated by hot embossing technology. Commercial available thermoplastic elastomer foils based on polyurethane (PU) in a thickness range of 100-600 μm have been used. Prior to the fabrication of the microfluidic devices the chemical resistance of the material against a wide range of standard biological buffer solutions and solvents had been analysed. We created systems of channels, reservoirs and holes for the connections to external capillaries by double-sided hot embossing with an alignment accuracy of +/- 3 micrometer. Closed channel structures were produced by an additional chemical bonding process of the embossed devices with another thermoplastic elastomer foil. The total volume of the fluidic cell was 2 μl/sensor for the use with SAW (surface-acoustic wave) sensor chip and about 0.2 μ/sensor for the impedance sensors. A novel multi-chamber fluidic device was successfully tested for in-situ immobilization of thrombin antibodies and Bovin Serum Albumin (BSA) on different sensor elements of the same sensor chip.

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References

1S-sens analytics (www.s-sens.com)Google Scholar
2 Schlensog, M., Gronewold, T.M.A., Tewes, M., Famulok, M., Quandt, E., Sensors and Actuators 101/3, 308–313 (2004).Google Scholar
3 Löhndorf, M., Malavé, A., Glass, S., Stoyanov, I., Tewes, M., Proc. of μ-TAS 2004, 2, 431–433 (2004).Google Scholar
4 Malavé, A., Gronewold, T.M.A., Tewes, M., Löhndorf, M., Micromechanical Engineering (2005) in press. Google Scholar
5 Heckele, M. and Schomburg, W.K., J. Micromech. Microeng. 14, R1–R14 (2004).Google Scholar
6 Gravesen, P., Branebjerg, J. and Jensen, O.S., J. Micromech. Microeng. 3, 168–182 (2003).Google Scholar
7 Domininghaus, H., Die Kunsstoffe und ihre Eigenschaften, 5th ed. (Springer-Verlag Berlin Heidelberg, 1998) pp16–32.Google Scholar
8 Spontak, R.J., Nikunj, P.P, J. Cur. Op. Col. & Int. Sc. 5, 334–341 (2000).Google Scholar
9 Hollande, S., Laurent, J.L. and Lebey, T., Polymer 39, 5343 (1998).Google Scholar
10 Qi, H.J. and Boyce, M.C., Mech. of mat.,(2004) (in press)Google Scholar
11 Holden, G., Legge, N.R., Quirk, R. and Schroeder, H.E., “Thermoplastic polyurethane elastomers,” Thermoplastic elastomers, 2nd ed., (Hanser/Gardner, 1996).Google Scholar
12Epurex Films GmbH & Co. (www.epurex.de).Google Scholar