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New biological labels based on functionalized YVO4: Eu nanoparticles

Published online by Cambridge University Press:  01 February 2011

D. Giaume,
V Buissette,
K. Lahlil,
T. Gacoin,
J.-P. Boilot,
D. Casanova,
E. Beaurepaire,
M.-P. Sauviat,
A. Mercuri and
A. Alexandrou
D. Giaume
Affiliation:
Laboratory of Condensed Matter Physics Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France
V Buissette
Affiliation:
Laboratory of Condensed Matter Physics Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France
K. Lahlil
Affiliation:
Laboratory of Condensed Matter Physics Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France
T. Gacoin
Affiliation:
Laboratory of Condensed Matter Physics Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France
J.-P. Boilot
Affiliation:
Laboratory of Condensed Matter Physics Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France
D. Casanova
Affiliation:
Laboratory for Optics and Biosciences, CNRS UMR 7645, INSERM U45L Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France
E. Beaurepaire
Affiliation:
Laboratory for Optics and Biosciences, CNRS UMR 7645, INSERM U45L Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France
M.-P. Sauviat
Affiliation:
Laboratory for Optics and Biosciences, CNRS UMR 7645, INSERM U45L Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France
A. Mercuri
Affiliation:
Laboratory for Optics and Biosciences, CNRS UMR 7645, INSERM U45L Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France
A. Alexandrou
Affiliation:
Laboratory for Optics and Biosciences, CNRS UMR 7645, INSERM U45L Ecole Polytechnique, route de Saclay, 91128 Palaiseau, France
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Abstract

Lanthanide-ion doped oxide (YVO4: Eu) nanoparticles were synthesized as aqueous colloids and functionalized by a bioactive silane shell to be used as fluorescent biological labels. Nanoparticles functionalized with guanidinium groups were able to act as artificial toxins which specifically target Na+ channels. They were individually detectable in live cardiac myocytes. Functionalized oxide nanoparticles appear as a new interesting tool, especially attractive for long-term single-molecule tracking due to their photo-stability and long luminescence lifetime.

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