Hostname: page-component-74656f9884-2dmjx Total loading time: 0 Render date: 2026-11-10T10:19:22.808Z Has data issue: false hasContentIssue false
  • English
  • Français

Optical Interferometric Biosensor with PMMA as Functional Layer

Published online by Cambridge University Press:  01 February 2011

Wenhui Wang ,
Xiaodong Ma ,
Lisa-Jo Clarizia ,
Xingwei Wang  and
Melisenda McDonald
Wenhui Wang
Affiliation:
wenhui_wang@uml.edu, UMass Lowell, ECE, Lowell, Massachusetts, United States
Xiaodong Ma
Affiliation:
xiaodong_ma@student.uml.edu, UMass Lowell, ECE, Lowell, Massachusetts, United States
Lisa-Jo Clarizia
Affiliation:
LisaJo_Clarizia@uml.edu, UMass Lowell, Chemistry, Lowell, Massachusetts, United States
Xingwei Wang
Affiliation:
xingwei_wang@uml.edu, UMass Lowell, ECE, Lowell, Massachusetts, United States
Melisenda McDonald
Affiliation:
Melisenda_McDonald@uml.edu, UMass Lowell, Chemistry, Lowell, Massachusetts, United States
Get access

Abstract

Silica-on-silicon label-free biosensor with PMMA (Poly(methyl methacrylate), as the functional layer was designed, fabricated, and tested. The sensor is based on Fabry Perot (FP) interferometry. Specific binding was tested with Human IgG and anti-Human IgG. Non-specific binding was tested with Human IgG and Mouse IgG. The testing results show that the sensor has a nearly six-fold greater response upon specific binding than upon non specific binding. Thermal and long term stability experiments show that the sensor is insensitive to the environment fluctuation. The fabrication process is simple without special surface treatment. In addition, this biosensor is inexpensive and easy to use.

Keywords

opticalsensorpolymer

Information

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1133: Symposium AA – Materials for Optical Sensors in Biomedical Applications , 2008 , 1133-AA03-02
Copyright
Copyright © Materials Research Society 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

REFERENCES

1. Lin, V.S.-Y., Motesharei, K., Dancil, K.-P. S., Sailor, M. J., and Ghadiri, M. R., “A Porous Silicon-Based Optical Interferometric Biosensor,” Science, vol. 278, p. 4, 1997.Google Scholar
2. Stefano, L. D., Arcari, P., Lamberti, A., Sanges, C., Rotiroti, L., Rea, I., and Rendina, I., “DNA Optical Detection Based on Porous Silicon Technology: from Biosensors to Biochips,” Sensors, vol. 7, pp. 214–221, 2007.Google Scholar
3. Wang, X., Cooper, K. L., Wang, A., Xu, J., Wang, Z., Zhang, Y., and Tu, Z., “Label-free DNA sequence detection using oligonucleotide functionalized optical fiber,” Applied Physics Letters, vol. 89, p. 163901, 2006.Google Scholar
4. Zhang, Y., Shibru, H., Cooper, K. L., and Wang, A., “Miniature fiber-optic multicavity Fabry–Perot interferometric biosensor,” Optics Letters, vol. 30, p. 3, 2005.Google Scholar
5. Yan, Z., Xiaopei, C., Yongxin, W., Cooper, K. L., and Anbo, W., “Microgap Multicavity Fabry-Perot Biosensor,” Lightwave Technology, Journal of, vol. 25, pp. 1797–1804, 2007.Google Scholar
6. Nikitin, P. I., Valeiko, M. V., and Gorshkov, B. G., “New direct optical biosensors for multi-analyte detection,” Sensors and Actuators B: Chemical, vol. 90, pp. 46–51, 2003.Google Scholar
7. Hänel, C. and Gauglitz, G., “Comparison of reflectometric interference spectroscopy with other instruments for label-free optical detection,” Analytical and Bioanalytical Chemistry, vol. 372, p. 9, 2002.Google Scholar
8. Gauglitz, G., “Direct optical sensors: principles and selected applications,” Analytical and Bioanalytical Chemistry vol. 381, pp. 141–155, 2005.Google Scholar
9. Hua, S., Kumar, D., and Lear, K. L., “Single cell detection using a microfluidic, passive, Fabry-Perot interferometer based biosensor,” in Lasers and Electro-Optics, 2005. (CLEO). Conference on, 2005, pp. 2151–2153 Vol. 3.Google Scholar
10. Mehlmann, M., Garvin, A. M., Steinwand, M., and Gauglitz, G. n., “Reflectometric interference spectroscopy combined with MALDI-TOF mass spectrometry to determine quantitative and qualitative binding of mixtures of vancomycin derivatives,” Analytical and bioanalytical chemistry vol. 382, p. 7, 2005.Google Scholar
11. Xu, J., Wang, X., Cooper, K. L., Pickrell, G. R., and Wang, A., “Miniature Temperature-Insensitive Fabry-Perot Fiber-Optic Pressure Sensor,” IEEE Photonics Technology Letters, vol. 18, pp. 1134–1336, 2006.Google Scholar
12. Cloarec, J. P., Martin, J. R., Polychronakos, C., Lawrence, I., Lawrence, M. F., and Souteyrand, E., “Functionalization of Si/SiO2 substrates with homooligonucleotides for a DNA biosensor,” Sensors and Actuators B: Chemical, vol. 58, pp. 394–398, 1999.Google Scholar
13. Fixe, A. F., Dufva, H. M., Telleman, P., and Stensen, C. B. V., “One-step immobilization of aminated and thiolated DNA onto Poly methyl-methacrylate (PMMA) substrates,” Lab on a Chip vol. 4, pp. 191–195, 2004.Google Scholar
14. Clarizia, LJA, Sok, D, Wei, M, Mead, J, Barry, C and McDonald, MJ, “Antibody orientation enhanced by selective polymer-protein noncovalent interactions” Anal. Bioanal. Chemistry (2009) In Press.Google Scholar
15. Marquette, C. A. and Blum, L. J., “State of the art and recent advances in immunoanalytical systems,” Biosensors and Bioelectronics, vol. 21, pp. 1424–1433, 2006.Google Scholar
16. Eun-Seok, K., Tae-Ho, L., and Byeong-Soo, B., “Measurement of the thermo-optic coefficients in sol-gel derived inorganic--organic hybrid material films,” Applied Physics Letters, vol. 81, pp. 1438–1440, 2002.Google Scholar