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Biodegradable Poly[bis(ethyl alanato)phosphazene] - Poly(lactide-co-glycolide) Blends: Miscibility and Osteocompatibility Evaluations

Published online by Cambridge University Press:  01 February 2011

Lakshmi S. Nair,
Jared D. Bender,
Anurima Singh,
Swaminathan Sethuraman,
Yaser E. Greish,
Paul W. Brown,
Harry R. Allcock and
Cato T. Laurencin
Lakshmi S. Nair
Affiliation:
Department of Orthopaedic Surgery, University of Virginia, VA 22903
Jared D. Bender
Affiliation:
Department of Chemistry, The Pennsylvania State University, PA 16802
Anurima Singh
Affiliation:
Department of Chemistry, The Pennsylvania State University, PA 16802
Swaminathan Sethuraman
Affiliation:
Department of Chemical Engineering, University of Virginia, VA 22904Department of Chemical Engineering, Drexel University, PA 19104
Yaser E. Greish
Affiliation:
Intercollege Materials Research Laboratory, The Pennsylvania State University, PA 16802
Paul W. Brown
Affiliation:
Intercollege Materials Research Laboratory, The Pennsylvania State University, PA 16802
Harry R. Allcock
Affiliation:
Department of Chemistry, The Pennsylvania State University, PA 16802
Cato T. Laurencin*
Affiliation:
Department of Orthopaedic Surgery, University of Virginia, VA 22903Department of Biomedical Engineering, University of Virginia, VA 22908Department of Chemical Engineering, University of Virginia, VA 22904
*
* e-mail: Laurencin@virginia.edu
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Abstract

We have previously demonstrated that blending biodegradable glycine co-substituted polyphosphazenes with poly(lactide-co-glycolide) (PLAGA) results in novel biomaterials with versatile properties. The study showed that the degradation rate of polyphosphazene/PLAGA blends can be effectively controlled by varying the blend composition while at the same time the degradation products of polyphosphazenes effectively neutralized the acidic degradation products of PLAGA. In the present study, novel blends of hydrophobic, biodegradable polyphosphazene, poly[bis(ethyl alanato) phosphazene] (PNEA) and PLAGA (LA: GA; 85:15) were developed as candidates for bone tissue engineering applications. Two different blend compositions were developed by blending PNEA and PLAGA having weight ratios of 25:75 (Blend-1) and 50:50 (Blend-2) by the mutual solvent technique using dichloromethane as the solvent. The miscibility of the blends was determined using differential scanning calorimetry (DSC), fourier transform-infrared spectroscopy (FT-IR), and scanning electron microscopy (SEM). Surface analysis of the blends by SEM revealed a smooth uniform surface for Blend-1, whereas Blend-2 showed evidence of phase separation. PNEA is not completely miscible with PLAGA, as evidenced from DSC and FT-IR measurements. The osteocompatibilities of Blend-1 and Blend-2 were compared to those of parent polymers by following the adhesion and proliferation of primary rat osteoblast cells on two dimensional (2-D) polymer and blend films over a 21 day period in culture. Blend films showed significantly higher cell numbers on the surface compared to PLAGA and PNEA films.

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References

REFERENCES

1. Vacanti, JP, CA, Vacanti. The history and scope of tissue engineering. RP, Lanza, R, Langer, J, Vacanti (Eds.), Principles of Tissue Engineering; Academic Press, New York, 2000, p 3–8 Google Scholar
2. Gross, KA, Rodriguez-Lorenzo, LM.. Biomaterials 2004; 25: 4955–4962 Google Scholar
3. Yang, L, Curr, Alexandridis P. Opin Coll Inter Sci 2000; 5: 132–143 Google Scholar
4. Sung, H, Meredith, C, Johnson, C, Galisa Z, S. Biomaterials 2004; 25: 5735–5742 Google Scholar
5. Allcock, HR. Chemistry and Applications of Polyphosphazenes; John Wiley & Sons, Hoboken, New Jersey, 2003 Google Scholar
6. Lakshmi, S, Katti, DS, Laurencin, CT., Advan Drug Delivery Rev. 2003; 55: 467–482 Google Scholar
7. Laurencin, CT, Norman, ME, Elgendy, HM, El-Amin, SF, Allcock, HR, Pucher, SR, Ambrosio, AA. J Biomed Mater Res 1993; 27: 963–973 Google Scholar
8. Laurencin, CT, El-Amin, SF, Ibim, SE, Willoughby, DA, Attawia, M, Allcock, HR, Ambrosio, AA. J Biomed Mater Res 1996; 30: 133–38Google Scholar
9. Ibim, SEM, Ambrosio, AMA, Kwon, MS, El-Amin, SF, Allcock, HR, Laurencin, CT. Biomaterials 1997; 18: 1565–69Google Scholar
10. Ambrosio, AMA, Allcock, HR, Katti, DS, Laurencin, CT. Biomaterials 2002; 23: 1667–72Google Scholar
11. Allcock, HR, Pucher, SR, Scopelianos, AG. Macromolecules 1994, 27: 1071–75Google Scholar
12. Bone, Schwartz E.. Freshney, RI (Ed.), Culture of animal cells; Wiley-Liss, New York, 1987, p 332 Google Scholar
13. Qiu, LY, Zhu, KJ. Polym Int 2000; 49: 1283–88Google Scholar