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CeO2 thin film for mediator-less glucose biosensors

Published online by Cambridge University Press:  01 February 2011

Shibu Saha,
S. K. Arya,
S. P. Singh,
B. D. Malhotra,
K. Sreenivas and
Vinay Gupta
Shibu Saha
Affiliation:
shibuss84@gmail.com, United States
S. K. Arya
Affiliation:
sunilarya333@gmail.com, National Physical Laboratory, Delhi, Delhi, India
S. P. Singh
Affiliation:
singh@uorm.edu, University of PuertoRico, Mayaguez, Puerto Rico
B. D. Malhotra
Affiliation:
bansi.malhota@gmail.com, National Physical Laboratory, Delhi, Delhi, India
K. Sreenivas
Affiliation:
kondepuddysreenivas@rediffmail.com, University of Delhi, Delhi, India
Vinay Gupta
Affiliation:
vgupta@physics.du.ac.in, University of Delhi, Delhi, India
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Abstract

Pulsed laser deposited cerium oxide (CeO2) nanoporous thin film on platinum (Pt) coated glass has been used for immobilization of glucose oxidase (GOx) by electrostatic interaction. Atomic force microscopy studies reveal the formation of nanoporous surface morphology of CeO2 thin film. Differential pulse voltammetric and optical measurements show that the GOx/CeO2/Pt bioelectrode is sensitive to the detection of glucose over the concentration upto 300 mg/dl. A low value of enzyme's kinetic parameter (Michaelis-Menten constant∼1.01 mM) indicates enhanced enzyme affinity of GOx to glucose.

Keywords

biomaterialbiomedicalcatalytic

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