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Development of a Single Molecular Tunnel-Current Identification method For Electrical Genome Sequencing

Published online by Cambridge University Press:  09 February 2015

Takahito Ohshiro,
Makusu Tsutsui,
Kazumichi Yokota,
Tomoji Kawai and
Masateru Taniguchi
Takahito Ohshiro
Affiliation:
Institute of Science and Industrial Research (ISIR), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka, Japan
Makusu Tsutsui
Affiliation:
Institute of Science and Industrial Research (ISIR), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka, Japan
Kazumichi Yokota
Affiliation:
Institute of Science and Industrial Research (ISIR), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka, Japan
Tomoji Kawai
Affiliation:
Institute of Science and Industrial Research (ISIR), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka, Japan
Masateru Taniguchi
Affiliation:
Institute of Science and Industrial Research (ISIR), Osaka University, Mihogaoka 8-1, Ibaraki, Osaka, Japan
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Abstract

We developed a tunnel-current based identification method by using nano-gap integrated devices. We performed electrical measurements for mono-nucleotide and oligo-nucleotide during its translocation of molecules between the nano-gap. Based on this determined electrical conductivity for single-nucleotides, we electrically identify the base-type in oligonucleotides, and found that this time-profiles represents the molecular translocation behaviors inside nano-gap. This method could be a promising for an electrical nucleotide sequencing methodology with label-free, high-speed, and low-cost.

Keywords

biomaterialnanostructurepolymer

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References

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