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Electron-beam Directed Layer-by-Layer Assembly of Dendrimer Scaffold for Biomolecule Patterning

Published online by Cambridge University Press:  01 February 2011

Parijat Bhatnagar,
Sonny S Mark,
Il Kim,
Hongyu Chen,
Brad Schmidt,
Michal Lipson and
Carl A Batt
Parijat Bhatnagar
Affiliation:
pb96@cornell.edu, Cornell University, Department of Biomedical Engineering, 309 Stocking Hall, Cornell University, Ithaca, NY, 14853, United States, 845-401-3427
Sonny S Mark
Affiliation:
ssm12@cornell.edu, Cornell University, Department of Microbiology, Ithaca, NY, 14853, United States
Il Kim
Affiliation:
ik58@cornell.edu, Cornell University, Department of Food Science, Ithaca, NY, 14853, United States
Hongyu Chen
Affiliation:
hc332@cornell.edu, Cornell University, Department of Food Science, Ithaca, NY, 14853, United States
Brad Schmidt
Affiliation:
bss15@cornell.edu, Cornell University, Department of Electrical and Computer Engineering, Ithaca, NY, 14853, United States
Michal Lipson
Affiliation:
lipson@ece.cornell.edu, Cornell University, Department of Electrical and Computer Engineering, Ithaca, NY, 14853, United States
Carl A Batt
Affiliation:
cab10@cornell.edu, Cornell University, Department of Biomedical Engineering, Microbiology, and Food Science, Ithaca, NY, 14853, United States
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Abstract

A method for patterning biomolecules using electron beam (e-beam) lithography has been developed. First, a non-biofouling poly (ethylene glycol) terminated self-assembled monolayer (SAM) was deposited on a silicon oxide substrate. This SAM was then ablated by e-beam to create patterns aligned with the pre-existing features on the wafer. Aldehyde-terminated polyamidoamine (PAMAM) dendrimers were assembled in a layer-by-layer fashion in the ablated patterns to allow the covalent immobilization of oligonucleotide probes. The aminated oligonucleotides were attached using Schiff base chemistry followed by reductive amination. The functionality of the attached oligonucleotides was demonstrated by the hybridization of fluorescently labeled complementary target oligonucleotides. The hybridized target oligonucleotides could be stripped and the regenerated surface bound probe oligonucleotides could be rehybridized with complementary target oligonucleotide.

Keywords

nanoscaleself-assemblysurface chemistry

Information

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 921: Symposium T – Nanomanufacturing , 2006, 0921-T04-08
Copyright
Copyright © Materials Research Society 2006

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References

1 Beske, O. E. and Goldbard, S., Drug Discovery Today 7 (18), S131 (2002).Google Scholar
2 Piehler, J., Current Opinion in Structural Biology 15 (1), 4 (2005).Google Scholar
3 Ramanathan, K., Bangar, M. A., Yun, M. et al., Journal of the American Chemical Society 127 (2), 496 (2005).Google Scholar
4 Schmidt, B., Almeida, V., Manolatou, C. et al., Applied Physics Letters 85 (21), 4854 (2004).Google Scholar
5 Cui, Y., Wei, Q. Q., Park, H. K. et al., Science 293 (5533), 1289 (2001); W. U. Wang, C. Chen, K. H. Lin et al., Proceedings of the National Academy of Sciences of the United States of America 102 (9), 3208 (2005).Google Scholar
6 Cui, H. M., Cruz-Correa, M., Giardiello, F. M. et al., Science 299 (5613), 1753 (2003).Google Scholar
7 Clarke, M. F., Nature 432 (7015), 281 (2004); S. K. Singh, C. Hawkins, I. D. Clarke et al., Nature 432 (7015), 396 (2004); S. Takayama, E. Ostuni, P. LeDuc et al., Nature 411 (6841), 1016 (2001); C. S. Chen, M. Mrksich, S. Huang et al., Science 276 (5317), 1425 (1997); S Raghavan and C. S. Chen, Advanced Materials 16 (15), 1303 (2004); A. Folch and M. Toner, Annual Review of Biomedical Engineering 2, 227 (2000); B. Vogelstein and K. W. Kinzler, Nature Medicine 10 (8), 789 (2004); M. Ferrari, Nature Reviews Cancer 5 (3), 161 (2005).Google Scholar
8 Koch, W.H., Nature Reviews Drug Discovery 3 (9), 749 (2004); D. Sidransky, Nature Reviews Cancer 2 (3), 210 (2002).Google Scholar
9 Markov, D. A., Swinney, K., and Bornhop, D. J., Journal of the American Chemical Society 126 (50), 16659 (2004).Google Scholar
10 Bhatnagar, Parijat, Mark, Sonny S., Kim, Il et al., Advanced Materials 18 (3), 315 (2006).Google Scholar
11 Stamou, D., Musil, C., Ulrich, W. P. et al., Langmuir 20 (9), 3495 (2004).Google Scholar
12 Xia, Y. N. and Whitesides, G. M., Annual Review of Materials Science 28, 153 (1998); G. M. Whitesides, E. Ostuni, S. Takayama et al., Annual Review of Biomedical Engineering 3, 335 (2001); J. C. McDonald and G. M. Whitesides, Accounts of Chemical Research 35 (7), 491 (2002).Google Scholar
13 Tormen, M., Borzenko, T., Steffen, B. et al., Applied Physics Letters 81 (11), 2094 (2002).Google Scholar
14 Ilic, B. and Craighead, H. G., Biomedical Microdevices 2 (4), 317 (2000).Google Scholar
15 Veiseh, M., Zareie, M. H., and Zhang, M. Q., Langmuir 18 (17), 6671 (2002).Google Scholar
16 Lee, K. B., Park, S. J., Mirkin, C. A. et al., Science 295 (5560), 1702 (2002); S. Kramer, R. R. Fuierer, and C. B. Gorman, Chemical Reviews 103 (11), 4367 (2003); D. J. Pena, M. P. Raphael, and J. M. Byers, Langmuir 19 (21), 9028 (2003); J. H. Lim, D. S. Ginger, K. B. Lee et al., Angewandte Chemie-International Edition 42 (20), 2309 (2003); L. M. Demers, D. S. Ginger, S. J. Park et al., Science 296 (5574), 1836 (2002).Google Scholar
17 Wadu-Mesthrige, K., Amro, N. A., Garno, J. C. et al., Biophysical Journal 80 (4), 1891 (2001); K. Wadu-Mesthrige, S. Xu, N. A. Amro et al., Langmuir 15 (25), 8580 (1999).Google Scholar
18 Benters, R., Niemeyer, C. M., and Wohrle, D., Chembiochem 2 (9), 686 (2001); Pathak, S., Singh, A. K., McElhanon, J. R. et al., Langmuir 20 (15), 6075 (2004); P. Angenendt, J. Glokler, J. Sobek et al., Journal of Chromatography A 1009 (1-2), 97 (2003); E. Trevisiol, V. Le Berre-Anton, J. Leclaire et al., New Journal of Chemistry 27 (12), 1713 (2003).Google Scholar
19 Tomalia, D. A., Naylor, A. M., and Goddard, W. A., Angewandte Chemie-International Edition in English 29 (2), 138 (1990).Google Scholar
20 Marsh, R. J., Jones, R. A. L., and Sferrazza, M., Colloids and Surfaces B-Biointerfaces 23 (1), 31 (2002).Google Scholar
21 Duchet, J., Chabert, B., Chapel, J. P. et al., Langmuir 13 (8), 2271 (1997); J. Duchet, J. F. Gerard, J. P. Chapel et al., Composite Interfaces 8 (3-4), 177 (2001).Google Scholar