Hostname: page-component-74656f9884-54qdv Total loading time: 0 Render date: 2026-11-11T00:35:05.896Z Has data issue: false hasContentIssue false
  • English
  • Français

Functionalized Waveguides for Biological Assays

Published online by Cambridge University Press:  01 February 2011

Aaron S. Anderson ,
Andrew M. Dattelbaum ,
Gabriel A. Montaño ,
Jurgen G. Schmidt ,
Jennifer S. Martinez ,
W. Kevin Grace ,
Karen M. Grace  and
Basil I. Swanson
Aaron S. Anderson
Affiliation:
Los Alamos National Lab, Center for Integrated Nanotechnologies, MS K771, Los Alamos National Lab, Los Alamos, NM, 87545, United States, 505-665-0142
Andrew M. Dattelbaum
Affiliation:
amdattel@lanl.gov, Los Alamos National Laboratory, MS K771, Los Alamos National Lab, Los Alamos, NM, 87545, United States
Gabriel A. Montaño
Affiliation:
gbmon@lanl.gov, Los Alamos National Laboratory, MS K771, Los Alamos National Lab, Los Alamos, NM, 87545, United States
Jurgen G. Schmidt
Affiliation:
jschmidt@lanl.gov, Los Alamos National Laboratory, MS K771, Los Alamos National Lab, Los Alamos, NM, 87545, United States
Jennifer S. Martinez
Affiliation:
jenm@lanl.gov, Los Alamos National Laboratory, MS K771, Los Alamos National Lab, Los Alamos, NM, 87545, United States
W. Kevin Grace
Affiliation:
wkg@lanl.gov, Los Alamos National Laboratory, MS K771, Los Alamos National Lab, Los Alamos, NM, 87545, United States
Karen M. Grace
Affiliation:
kgrace@lanl.gov, Los Alamos National Laboratory, MS K771, Los Alamos National Lab, Los Alamos, NM, 87545, United States
Basil I. Swanson
Affiliation:
basil@lanl.gov, Los Alamos National Laboratory, MS K771, Los Alamos National Lab, Los Alamos, NM, 87545, United States
Get access

Abstract

We report here a procedure for the functioalization of SiO2-coated, SiONx waveguides for biological assays. Surface functionalization occurs by self-assembly of an amine-terminated silane monolayer on the waveguide, followed by partial chemical modification with functionalized polyethylene glycol (PEG) groups. Functionalized surfaces were characterized by atomic force microscopy and contact angle measurements. When combined with a BSA blocking step, these functional PEG surfaces significantly reduced non-specific binding and allowed for specific binding to occur. An antibody sandwich assay for detection of Bacillus anthracis protective antigen was used to validate these surfaces for sensing applications.

Keywords

sensorbiologicalself-assembly

Information

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 950: Symposium D – Biosurfaces and Biointerfaces , 2006 , 0950-D04-38
Copyright
Copyright © Materials Research Society 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

REFERENCES

1. Castner, D. G. and Ratner, B. D., Surf. Sci. 500, 28 (2002).Google Scholar
2. Martinez, J. S., Grace, W. K., Grace, K. M., Hartman, N. and Swanson, B. I., J. Mater. Chem. 15, 4639 (2005).Google Scholar
3. Grace, K. M. et al., Proc. SPIE-Int. Soc. Opt. Eng. 5269, 55 (2004).Google Scholar
4. Kelly, D. et al., Opt. Lett. 24, 1723 (1999).Google Scholar
5. Palegrosdemange, C., Simon, E. S., Prime, K. L. and Whitesides, G. M., J. Am. Chem. Soc. 113, 12 (1991).Google Scholar
6. Prime, K. L. and Whitesides, G. M., Science 252, 1164 (1991).Google Scholar
7. Lee, S. W. and Laibinis, P. E., Biomaterials 19, 1669 (1998).Google Scholar
8. Papra, A., Gadegaard, N. and Larsen, N. B., Langmuir 17, 1457 (2001).Google Scholar
9. Zhang, M. Q., Desai, T. and Ferrari, M., Biomaterials 19, 953 (1998).Google Scholar
10. Dalsin, J. L. et al., Langmuir 21, 640 (2005).Google Scholar
11. Huang, N. P. et al., Langmuir 18, 252 (2002).Google Scholar
12. Zhen, G. L. et al., Langmuir 20, 10464 (2004).Google Scholar
13. Zhen, G. L. et al., Adv. Funct. Mater. 16, 243 (2006).Google Scholar
14. Ma, H. W., Hyun, J. H., Stiller, P. and Chilkoti, A., Adv. Mater. 16, 338 (2004).Google Scholar
15. Meagher, R. J., Seong, J., Laibinis, P. E. and Barron, A. E., Electrophoresis 25, 405 (2004).Google Scholar
16. Pasche, S., Voros, J., Griesser, H. J., Spencer, N. D. and Textor, M., J. Phys. Chem. B 109, 17545 (2005).Google Scholar
17. Alcantar, N. A., Aydil, E. S. and Israelachvili, J. N., J. Biomed. Mater. Res. 51, 343 (2000).Google Scholar