Hostname: page-component-7b7567c8c-jswmv Total loading time: 0 Render date: 2026-11-15T22:24:44.044Z Has data issue: false hasContentIssue false
  • English
  • Français

Integrating Colloidal Quantum Dots with Porous Silicon for HighSensitivity Biosensing

Published online by Cambridge University Press:  21 March 2011

Girija Gaur,
Dmitry Koktysh and
Sharon M. Weiss
Girija Gaur
Affiliation:
Department of Electrical Engineering and Computer Science, Vanderbilt University, Nashville, TN 37235, U.S.A.
Dmitry Koktysh
Affiliation:
Department of Chemistry, Vanderbilt Institute of Nanoscale Science and Engineering, Vanderbilt University, Nashville, TN 37235, USA
Sharon M. Weiss
Affiliation:
Department of Electrical Engineering and Computer Science, Vanderbilt University, Nashville, TN 37235, U.S.A.
Get access

Abstract

We aim to utilize the high surface area of a porous silicon (PSi) matrixcoupled with semiconductor quantum dot (QD) amplifiers for ultrasensitiveoptical detection of small biomolecules using a dual-mode detection scheme.In our system, QDs attached to the target biomolecule serve as signalamplifiers by providing an additional refractive index increase beyond thatof the smaller target molecules. The strong photoluminescence (PL) from theQDs serves as a secondary indication of target molecule attachment in thepores. A resulting increase in optical thickness of ∼190 nm and detectionsensitivity of ∼700 nm/RIU have been demonstrated for attachment ofglutathione capped CdTe QDs in the porous silicon matrix. Reflectance and PLmeasurements, combined with simulations, have been used to characterize thesurface area coverage of the QDs within the porous framework, which isestimated at 10% for glutathione capped CdTe QDs.

Keywords

sensorluminescenceoptical

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

REFERENCES

[1] Rossi, A. M., et al. , “Porous silicon biosensor for detection of viruses,” Biosens. Bioelectron., vol. 23, pp. 741–745, 2007.Google Scholar
[2] Dancil, K.-P. S., et al. ., “A Porous Silicon Optical Biosensor: Detection of Reversible Binding of IgG to a Protein A-Modified Surface,” Journal of the American Chemical Society, vol. 121, pp. 7925–7930, 1999.Google Scholar
[3] Orosco, M. M., et al. ., “Real-time monitoring of enzyme activity in a mesoporous silicon double layer,” Nat Nano, vol. 4, pp. 255–258, 2009.Google Scholar
[4] Rong, G., et al. ., “Label-free porous silicon membrane waveguide for DNA sensing,” Appl. Phys. Lett., vol. 93, p. 161109, 2008.Google Scholar
[5] Homola, J., et al. ., “Surface plasmon resonance sensors: review,” Sensors and Actuators B: Chemical, vol. 54, pp. 3–15, 1999.Google Scholar
[6] Nassar, A. F., et al. ., Drug Metabolism Handbook: Concepts and Applications: John Wiley & Sons, Inc., New Jersey., 2009.Google Scholar
[7] Hudson, V. M., “Rethinking cystic fibrosis pathology: the critical role of abnormal reduced glutathione (GSH) transport caused by CFTR mutation,” Free Radical Biology and Medicine, vol. 30, pp. 1440–1461, 2001.Google Scholar
[8] Rong, G., et al. ., “High sensitivity sensor based on porous silicon waveguide,” in Mat. Res. Soc. Symp. Proc., 2006, pp. 0934-I10-04.Google Scholar
[9] Pacholski, C., et al. ., “Biosensing using porous silicon double-layer interferometers: reflective interferometric fourier transform spectroscopy,” J. Am. Chem. Soc., vol. 127, pp. 11636–11645, 2005.Google Scholar
[10] Jiao, Y., et al. ., “Dual-mode sensing platform based on colloidal gold functionalized porous silicon,” Applied Physics Letters, vol. 97, pp. 153125–3, 2010.Google Scholar