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Lab-on-Glass System for DNA Analysis using Thin and Thick Film Technologies

Published online by Cambridge University Press:  31 January 2011

Domenico Caputo,
Matteo Ceccarelli,
Giampiero de Cesare,
Augusto Nascetti and
Riccardo Scipinotti
Domenico Caputo
Affiliation:
caputo@die.uniroma1.it, Sapienza University of Rome, Electronic Engineering, via Eudossiana 18, Rome, 00184, Italy
Matteo Ceccarelli
Affiliation:
ceccarelli@die.uniroma1.it, Sapienza University of Rome, Electronic Engineering, Rome, Italy
Giampiero de Cesare
Affiliation:
decesare@die.uniroma1.it, Sapienza University of Rome, Electronic Engineering, Rome, Italy
Augusto Nascetti
Affiliation:
augusto.nascetti@uniroma1.it, Sapienza University of Rome, Aerospace and Astronautics, Rome, Italy
Riccardo Scipinotti
Affiliation:
scipinotti@die.uniroma1.it, Sapienza University of Rome, Electronic Engineering, Rome, Italy
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Abstract

In this paper, we present a compact lab-on-chip system suited for label-free DNA analysis. The system can be fabricated on a conventional microscope glass slide using thin-film and thick-film technologies. It integrates a heating chamber, an electrowetting-based droplet handling system and a hydrogenated amorphous silicon (a-Si:H) photosensor array for DNA detection. At this stage of research we have designed and tested the individual functional units. The heating chamber incorporates a thin metal film heater optimized for uniform temperature distribution on a 1cm2 area. A forward-biased a-Si:H p-i-n junction is used for temperature monitoring, achieving a linear temperature dependence with -3.3 mV/K sensitivity. The droplet-handling unit, relying on the electrowetting method, is designed to move the sample from the heating chamber to the sensor array. The unit includes a set of metal pads beneath a layer of PDMS that provides both the electric insulation of the electrodes and the hydrophobic surface needed by the electrowetting technique. The UV sensor array allows measuring the DNA absorbance variation at 254nm related to the hybridization between probe-molecules contained in the sample and reference target molecules immobilized on the sensor surface. A preliminary test to detect the hybridization between a 25-mer single-stranded oligonucleotides and denaturated pBR 322 4162-mer single-stranded oligonucleotides has been carried out successfully.

Keywords

thin filmamorphousbiological

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