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Localized Surface Plasmon Resonance Biosensor Using Ag Nanostructured Films Fabricated by a Reduction Method

Published online by Cambridge University Press:  01 February 2011

Tomofumi Arai,
Penmetcha K. R. Kumar,
Koichi Awazu and
Junji Tominaga
Tomofumi Arai
Affiliation:
t-arai@aist.go.jp, National Institute of Advanced Industrial Science and Technology (AIST), Center for Applied Near Field Optics Research (CAN-FOR), 1-1-1 Higashi, Tsukuba, N/A, 305-8562, Japan, +81-29-861-2911, +81-29-861-2939
Penmetcha K. R. Kumar
Affiliation:
pkr-kumar@aist.go.jp, National Institute of Advanced Industrial Science and Technology (AIST), Functional Nucleic Acids Group, Institute for Biological Resources and Functions, 1-1-1 Higashi, Tsukuba, N/A, 305-8562, Japan
Koichi Awazu
Affiliation:
k.awazu@aist.go.jp, National Institute of Advanced Industrial Science and Technology (AIST), Center for Applied Near Field Optics Research (CAN-FOR), 1-1-1 Higashi, Tsukuba, N/A, 305-8562, Japan
Junji Tominaga
Affiliation:
j-tominaga@aist.go.jp, National Institute of Advanced Industrial Science and Technology (AIST), Center for Applied Near Field Optics Research (CAN-FOR), 1-1-1 Higashi, Tsukuba, N/A, 305-8562, Japan
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Abstract

In this paper, an optical biosensor based on the localized surface plasmon resonance (LSPR) of Ag nanostructured films is proposed and demonstrated. The Ag nanostructured films, which are fabricated by the reduction of AgOx thin films, exhibit a strong LSPR at wavelengths around 370 nm in an air environment. The reflectance spectra of the Ag nanostructured film represent that the shift in the LSPR wavelength follows a linear dependence on the refractive index of the surrounding medium. By varying the concentration of streptavidin solution, we demonstrate that the Ag nanostructured films functionalized with thiol and biotin molecules can sensitively detect a binding event between biotin and streptavidin molecules.

Keywords

Agnanostructuresensor

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