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Microfabricated 3D Scaffolds for Tissue Engineering Applications

Published online by Cambridge University Press:  01 February 2011

Alvaro Mata,
Aaron J. Fleischman and
Shuvo Roy
Alvaro Mata
Affiliation:
Department of Biomedical Engineering, Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio 44195, U.S.A
Aaron J. Fleischman
Affiliation:
Department of Biomedical Engineering, Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio 44195, U.S.A
Shuvo Roy
Affiliation:
Department of Biomedical Engineering, Lerner Research Institute, The Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio 44195, U.S.A
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Abstract

Microfabrication and soft lithographic techniques are combined to develop three-dimensional (3D) polydimethylsiloxane (PDMS) scaffolds comprising multiple levels of meandering pore geometry textured with 10 μm posts. Both micro-architecture and surface micro-textures have been shown to selectively stimulate cell and tissue behavior. To achieve a 3D scaffold with precise micro-architecture and surface micro-textures, 100 μm thick PDMS films were manufactured using a stacking technique to realize a 66% porous 3D structure with 200 × 400 μm horizontal through holes, 300 μm diameter vertical through holes and 71% surface coverage with 10 μm diameter and 10 μm high posts. Each PDMS porous film level was manufactured by the dual-sided molding of uncured PDMS between a three level SU-8 photoresist mold (of 200, 10, and 100 μm thick features) and a PDMS mold with 10 μm deep micro-textures. Dual-sided molding was achieved using a custom motion control mechanical jig that allowed relative mold alignment to within ∼ ±10 μm.

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References

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