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New Atomic Force Microscopy Based Astrocyte Cell Shape Index

Published online by Cambridge University Press:  20 March 2013

Volkan Mujdat Tiryaki,
Usienemfon Adia-Nimuwa,
Steven Allen Hartz,
Kan Xie,
Virginia M. Ayres,
Ijaz Ahmed and
David I. Shreiber
Volkan Mujdat Tiryaki
Affiliation:
Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI 48824, USA
Usienemfon Adia-Nimuwa
Affiliation:
Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI 48824, USA
Steven Allen Hartz
Affiliation:
Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI 48824, USA
Kan Xie
Affiliation:
Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI 48824, USA
Virginia M. Ayres
Affiliation:
Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI 48824, USA
Ijaz Ahmed
Affiliation:
Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
David I. Shreiber
Affiliation:
Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ 08854, USA
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Abstract

A new three-dimensional cell shape index using the AFM height images of cells cultured on cell substrates was defined. The new cell shape index revealed quantitative cell spreading information of cells that is not included in the conventional cell shape index. The quantitative morphometry of rat cerebral cortical astrocytes cultured on four different kinds of cell substrates were investigated using the conventional and the new cell shape index, and the results were compared. The new cell shape index showed the quantitative astrocyte spreading and stellation behavior that agrees with the AFM height images of astrocytes.

Keywords

biomaterialmorphologyscanning probe microscopy (SPM)

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