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Self-Assembled Monolayers as Models for Studying Protein Adsorption to Polymer Surfaces

Published online by Cambridge University Press:  21 February 2011

Kevin L. Prime and
George M. Whttesides
Kevin L. Prime
Affiliation:
Department of Chemistry, Harvard University, Cambridge, MA 02138
George M. Whttesides
Affiliation:
Department of Chemistry, Harvard University, Cambridge, MA 02138
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Abstract

Self-assembled monolayers (SAMs) of functionalized alkanethiolates on gold are a well-characterized system for studying the interfacial properties of organic materials. We have used SAMs as models for the surfaces of organic polymers and used mem to study the adsorption of proteins onto organic materials. We have formed SAMs from mixtures of alkanethiols in which one alkanethiol is hydrophobic and the other is terminated by a short (2 ≤ n ≤ 17) oligomer of poly(ethylene oxide). These “mixed” SAMs effectively resist the adsorption of fibrinogen from moderately concentrated (1 mg/mL) solutions. Protein adsorption begins when < 5% of the accessible area of the surface consists of hydrophobic groups. These findings suggest that real protein-resistant monolayers must present an almost defect-free surface of oligo(ethylene oxide) groups in order to eliminate adsorption.

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Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 237: Symposium Ca – Interface Dynamics and Growth , 1991, 311
Copyright
Copyright © Materials Research Society 1992

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