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Structure-property-function relationships in triple-helical collagen hydrogels

Published online by Cambridge University Press:  18 December 2012

Giuseppe Tronci,
Amanda Doyle,
Stephen J. Russell and
David J. Wood
Giuseppe Tronci
Affiliation:
Biomaterials and Tissue Engineering Research Group, Leeds Dental Institute, University of Leeds, Leeds LS2 9LU, United KingdomNonwoven Research Group, Centre for Technical Textiles, University of Leeds, Leeds LS2 9JT, United Kingdom
Amanda Doyle
Affiliation:
Biomaterials and Tissue Engineering Research Group, Leeds Dental Institute, University of Leeds, Leeds LS2 9LU, United KingdomNonwoven Research Group, Centre for Technical Textiles, University of Leeds, Leeds LS2 9JT, United Kingdom
Stephen J. Russell
Affiliation:
Nonwoven Research Group, Centre for Technical Textiles, University of Leeds, Leeds LS2 9JT, United Kingdom
David J. Wood
Affiliation:
Biomaterials and Tissue Engineering Research Group, Leeds Dental Institute, University of Leeds, Leeds LS2 9LU, United Kingdom
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Abstract

In order to establish defined biomimetic systems, type I collagen was functionalised with 1,3-Phenylenediacetic acid (Ph) as aromatic, bifunctional segment. Following investigation on molecular organization and macroscopic properties, material functionalities, i.e. degradability and bioactivity, were addressed, aiming at elucidating the potential of this collagen system as mineralization template. Functionalised collagen hydrogels demonstrated a preserved triple helix conformation. Decreased swelling ratio and increased thermo-mechanical properties were observed in comparison to state-of-the-art carbodiimide (EDC)-crosslinked collagen controls. Ph-crosslinked samples displayed no optical damage and only a slight mass decrease (∼ 4 wt.-%) following 1-week incubation in simulated body fluid (SBF), while nearly 50 wt.-% degradation was observed in EDC-crosslinked collagen. SEM/EDS revealed amorphous mineral deposition, whereby increased calcium phosphate ratio was suggested in hydrogels with increased Ph content. This investigation provides valuable insights for the synthesis of triple helical collagen materials with enhanced macroscopic properties and controlled degradation. In light of these features, this system will be applied for the design of tissue-like scaffolds for mineralized tissue formation.

Keywords

biomaterialbiomimetic (chemical reaction)morphology

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References

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