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Sub-micron Patterning on Polymer Films for Protein Arrays

Published online by Cambridge University Press:  01 February 2011

Karen L. Christman,
Michael V. Requa,
Vanessa D. Enriquez-Rios,
Paula Mendes,
J. Fraser Stoddart,
Kimberley L. Turner and
Heather D. Maynard
Karen L. Christman
Affiliation:
christman@chem.ucla.edu, University of California Los Angeles, 607 Charles E. Young Dr. East, Los Angeles, CA, 90095-1569, United States
Michael V. Requa
Affiliation:
requa@engineering.ucsb.edu
Vanessa D. Enriquez-Rios
Affiliation:
ver@chem.ucla.edu
Paula Mendes
Affiliation:
mendes@chem.ucla.edu
J. Fraser Stoddart
Affiliation:
stoddart@chem.ucla.edu
Kimberley L. Turner
Affiliation:
turner@engineering.ucsb.edu
Heather D. Maynard
Affiliation:
maynard@chem.ucla.edu

Abstract

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Patterning proteins at the sub-micron and nanoscale has many uses, including fabrication of protein arrays for diagnostic and sensor applications. Many groups have reported micron scale protein patterns using photolithography; however, smaller scales have not been realized. In this study, we demonstrate sub-micron protein patterns using photolithography. Patterning is achieved by chemical transformation of pH-reactive polymer films. Site-specific immobilization of streptavidin within a protein resistant background is demonstrated. This methodology could be utilized for the development of high density proteins arrays for biotechnology applications.

Keywords

polymerfilmnanoscale

References

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