Hostname: page-component-7b7567c8c-2n9c9 Total loading time: 0 Render date: 2026-11-14T02:17:15.873Z Has data issue: false hasContentIssue false
  • English
  • Français

An immunocytochemical study with Fasciola hepatica

Published online by Cambridge University Press:  06 April 2009

E. Thorpe
E. Thorpe
Affiliation:
Imperial Chemical Industries Limited, Pharmaceuticals Division, Alderley Park, Cheshire
Get accessRights & Permissions [Opens in a new window]

Extract

The results are described of a study of the distribution of the antigens in F. hepatica at various stages of development, and in the liver and spleen of experimentally infected rats using globulins labelled with fluorescein isothiocyanate, from rats and sheep infected with F. hepatica.

Possible uses of this technique in aiding the diagnosis of fascioliasis are indicated.

I wish to thank Dr A. W. J. Broome for a supply of the metacercariae of F. hepatica, and Dr J. S. S. Lowe who kindly isolated and labelled the globulins with fluorescein isothiocyanate. Miss Enid Eccleston gave valuable technical assistance.

Information

Type
Research Article
Information
Parasitology , Volume 55 , Issue 2 , May 1965, pp. 209 - 214
Copyright
Copyright © Cambridge University Press 1965

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

Anderson, R. I., Sadun, E. H. & Williams, J. S. (1961). A technique for the use of minute amounts of dried blood in the fluorescent antibody test for schistosomiasis. Expl Parasit. 11, 111–6.Google Scholar
Andrade, Z. A., Paronetto, F. & Popper, H. (1961). Immunocytochemical studies in schistosomiasis. Am. J. Path. 39, 589–95.Google ScholarPubMed
Benex, J. & Deschiens, R. (1961). L'evolution comparée du proteinogramme et de la réaction de fixation du complement dans l'immunisation expérimentale du lapin avec des antigènes parasitaires. C. r. hebd. Séanc. Acad. Sci., Paris, 252, 951–53.Google Scholar
Benex, J., Lamy, L. & Gledel, J. (1959). Étude de la réaction de fixation du complement à l'antigène distomien chez le mouton. Bull. Soc. Path. exot. 52, 83–7.Google Scholar
Healy, G. R. (1955). Studies on immunity to Fasciola hepatica in rabbits. J. Parasit. 41, Sect. 2 (Suppl.), 25.Google Scholar
Kekwick, R. A. (1940). The serum proteins in multiple myelomatosis. Biochem. J. 34, 1248–56.CrossRefGoogle ScholarPubMed
Kohn, J. (1959). A simple method for the concentration of fluids containing protein. Nature, Lond., 183, 1055.CrossRefGoogle ScholarPubMed
Minning, von W. & Fuhrmann, G. (1955). Protein-, Kohlehydrat-, und Lipoid-Fraktionen von Fasciola hepatica als KBR-Antigene. Z. Tropenmed. u. Parasit. 6, 92–9.Google Scholar
Riggs, J. L., Seiwald, R. J., Burckhalter, J. H., Downs, C. M. & Metcalf, T. G. (1958). Isothiocyanate compounds as fluorescent labelling agents for immune serum. Amer. J. Path. 34, 1081–91.Google Scholar
Sadun, E. H., Anderson, R. I. & Williams, J. S. (1962). The nature of fluorescent antibody reactions in infectious and artificial immunisations with Schistosoma mansoni. Bull. Wld Hlth Org. 27, 151–9.Google Scholar
Stephenson, W. (1947). Physiological and histochemical observations on the adult liver fluke. II. Feeding. Parasitology, 38, 123–7.CrossRefGoogle Scholar
Urquhart, G. M., Mulligan, W. & Jennings, F. W. (1954). Artificial immunity to Fasciola hepatica in rabbits. 1. Some studies with protein antigens of F. hepatica. J. infect. Dis. 94, 126–33.CrossRefGoogle Scholar
Wikerhauser, T. (1961). Immunobiologic diagnosis of fascioliasis. II. The in vitro action of immune serum on the young parasitic stage of F. hepatica—a new precipitin test for fascioliasis. Vet. Arhiv, 31, 71–80.Google Scholar