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The application of recombinant DNA technology to problems of helminth identification

Published online by Cambridge University Press:  23 August 2011

D. Rollinson,
T. K. Walker and
A. J. G. Simpson
D. Rollinson
Affiliation:
Department of Zoology, British Museum (Natural History), Cromwell Road, London SW7 5BD
T. K. Walker
Affiliation:
Department of Zoology, British Museum (Natural History), Cromwell Road, London SW7 5BD
A. J. G. Simpson
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
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Extract

During the past decade, enormous technological developments have occurred in biology that have led to significant and revolutionary advances. New techniques of DNA cloning, restriction-enzyme analyses and nucleotide sequencing are providing a mass of data concerning the genomes of a wide variety of organisms. Such insights are having a great impact upon many areas of biological investigation, and would seem to be of considerable potential value for studies of taxonomy and population biology. The application of these new approaches to the characterization and identification of parasitic helminths has only recently begun, but they promise to become powerful additional tools for this purpose. Better methods of characterization are required for more precise definition of the parasites of man and domestic animals and for determining vectors and intermediate hosts as well as possible animal reservoir hosts. Moreover, a greater understanding of the genetic diversity of parasitic organisms is required since many helminths, which are morphologically similar, show marked differences in epidemiologically significant factors such as infectivity, pathogenicity, immunogenicity and drug sensitivity.

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