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Properties of DNA from some malarial parasites

Published online by Cambridge University Press:  06 April 2009

W. E. Gutteridge,
P. I. Trigg and
D. H. Williamson
W. E. Gutteridge
Affiliation:
Divisions of Parasitology and Microbiology, National Institute for Medical Research, Mill Hill, London, N.W. 7
P. I. Trigg
Affiliation:
Divisions of Parasitology and Microbiology, National Institute for Medical Research, Mill Hill, London, N.W. 7
D. H. Williamson
Affiliation:
Divisions of Parasitology and Microbiology, National Institute for Medical Research, Mill Hill, London, N.W. 7
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Extract

The base compositions of DNA from some species of malarial parasite have been estimated by measurement of density in CsCl gradients and of thermal denaturation temperatures. Species from avian (Plasmodium gallinaceum) and rodent (P. berghei and P. vinckei) hosts – often used as model systems in malarial studies – contain single DNA components with base compositions of about 20% G + C. By contrast, species from primate hosts (P. knowlesi and P. falciparum) contain major DNA components of about 40% G + C and minor ones of about 20% G + C. These differences could be reflexions of markedly different genomes within these two groups of the genus Plasmodium.

Apart from differences in base composition, the physico-chemical properties of the main components of DNA from primate and rodent malarial parasites are very similar to those of DNA from mammalian cells. DNA from avian malarial parasites appears to have some unusual properties.

One of us (P.I.T.) received financial assistance from the World Health Organization. We thank Dr F. Hawking and Dr J. Williamson for many helpful discussions, Dr W. H. G. Richards, Dr A. Voller and Dr D. C. Warhurst for the provision of some of the strains and Miss Jane Dunnett, Mrs A. C. Gutteridge and Mr T. Scott-Finnigan for technical assistance.

Information

Type
Research Article
Information
Parasitology , Volume 62 , Issue 2 , April 1971, pp. 209 - 219
Copyright
Copyright © Cambridge University Press 1971

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References

REFERENCES

Adam, K. M. G., Blewett, D. A. & Flamm, W. G. (1969). The DNA of Acanthamoeba spp. A method for extraction and its characterisation. Journal of Protozoology 16, 6–12.CrossRefGoogle Scholar
Bahr, G. F. (1966). Quantitative cytochemical study of erythrocytic stages of Plasmodium lophurae and Plasmodium berghei, Military Medicine, 131, Supplement, 1064–70.Google Scholar
Bernardi, G., Carnevali, F., Nicolaieff, A., Piperno, G. & Tecce, G. (1968). Separation and characterisation of a satellite deoxyribonucleic acid from a yeast cytoplasmic ‘petite’ mutant. Journal of Molecular Biology 37, 493–505.Google Scholar
Bernardi, G., Faures, M., Piperno, G. & Slonimski, P. P. (1970). Mitochondrial DNA's from respiratory-sufficient and cytoplasmic respiratory-deficient mutant yeast. Journal of Molecular Biology 48, 23–42.CrossRefGoogle ScholarPubMed
Brown, I. N., Brown, K. N. & Hills, L. A. (1966). The separation of Plasmodium knowlesi from host cells. Transactions of the Royal Society for Tropical Medicine and Hygiene 60, 3.CrossRefGoogle Scholar
Burchall, J. J. & Hitchings, G. H. (1965). Inhibition binding analysis of dihydrofolate reductases from various species. Molecular Pharmacology 1, 126–36.Google Scholar
Burton, K. (1956). A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochemical Journal 62, 315–23.CrossRefGoogle ScholarPubMed
Dixon, H. (1966). Blood platelets as a source of enzyme activity in washed trypanosome suspensions. Nature 210, 428.Google Scholar
Ferone, R., Burchall, J. J. & Hitchings, G. H. (1969). Plasmodium berghei dihydrofolate reductase. Isolation, properties and inhibition by antifolates. Molecular Pharmacology 5, 49–59.Google Scholar
Gutteridge, W. E. & Trigg, P. I. (1970 a). The base compositions of DNA from some malarial parasites. Transactions of the Royal Society for Tropical Medicine and Hygiene 64, 11–12.Google Scholar
Gutteridge, W. E. & Trigg, P. I. (1970 b). Incorporation of radioactive precursors into DNA and RNA of Plasmodium knowlesi in vitro. The Journal of Protozoology 17, 89–96.Google Scholar
Gutteridge, W. E., Trigg, P. I. & Williamson, D. H. (1969). Base compositions of DNA from some malaria parasites. Nature 224, 1210–11.CrossRefGoogle Scholar
Howells, R. E. & Fullard, F. J. (1970). Mitochondrial changes during the malarial life cycle. Transactions of the Royal Society for Tropical Medicine and Hygiene 64, 6.Google ScholarPubMed
Marmur, J. (1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. Journal of Molecular Biology 3, 208–18.Google Scholar
Marmur, J. & Doty, P. (1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. Journal of Molecular Biology 5, 109–18.CrossRefGoogle ScholarPubMed
Newton, B. A. (1968). Biochemical peculiarities of trypanosomatid flagellates. Annual Review of Microbiology 22, 109–30.Google Scholar
Riou, R. & Pautrizel, R. (1969). Nuclear and kinetoplastic DNA from trypanosomes. Journal of Protozoology 16, 509–13.Google Scholar
Rudzinska, M. A. & Trager, W. (1968). The fine structure of trophozoites and gametocytes in Plasmodium coatneyi. Journal of Protozoology 15, 73–88.Google Scholar
Schildkraut, C. L., Marmur, J. & Doty, P. (1962). Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. Journal of Molecular Biology 4, 430–43.Google Scholar
Sevag, M. G., Lackman, D. B. & Smolens, J. (1938). The isolation of the components of streptococcal nucleoproteins in serologically active form. Journal of Biological Chemistry 124, 425–36.CrossRefGoogle Scholar
Sueoka, N. (1961). Variation and heterogeneity of base composition of deoxyribonucleic acid; a compilation of old and new data. Journal of Molecular Biology 3, 31–40.CrossRefGoogle Scholar
Suyama, Y. (1966). Mitochondrial DNA of Tetrahymena. Its partial physical characterisation. Biochemistry 5, 2214–21.CrossRefGoogle Scholar
Trigg, P. I., Brown, I. N., Gutteridge, W. E., Hockley, D. J. & Williamson, J. (1970). The preparation of free malaria parasites by nitrogen cavitation. Transactions of the Royal Society for Tropical Medicine and Hygiene 64, 2–3.Google Scholar
Walsh, C. J. & Sherman, I. W. (1968). Isolation, characterization and synthesis of DNA from a malaria parasite. Journal of Protozoology 15, 503–8.Google Scholar
Whitfield, P. R. (1953). The uptake of radioactive phosphorus by erythrocytic stages of Plasmodium berghei. Australian Journal of Biological Sciences 6, 591–6.Google Scholar
Williamson, J. & Cover, B. (1966). Separation of blood cell free trypanosomes and malaria parasites on a sucrose gradient. Transactions of the Royal Society for Tropical Medicine and Hygiene 60, 425–7.CrossRefGoogle Scholar