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Some cytochemical observations on Toxoplasma gondii

Published online by Cambridge University Press:  06 April 2009

B. Dasgupta and
C. Kulasiri
B. Dasgupta
Affiliation:
Department of Parasitology, London School of Hygiene and Tropical Medicine, London, England
C. Kulasiri
Affiliation:
Department of Parasitology, London School of Hygiene and Tropical Medicine, London, England
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Extract

A positive Feulgen reaction has been seen in all stages of Toxoplasma gondii. The intense basophilia seen in the intracellular and the extracellular stages is removable by treatment with ribonuclease. Granules which reacted positively with the periodic acid-Schiff technique were found in abundance in the cytoplasm of the cysts, whereas the same granules were not universally present in the intracellular and the extracellular stages at all days of infection. The cyst wall stained with this technique with varying degree of intensity.

It is thought that the variations seen in the individual parasites may be attributed to various causes, for example, strain differences, differences in the reaction of the host to the parasite, differences in the period of infection or to other unknown causes.

Information

Type
Research Article
Information
Parasitology , Volume 49 , Issue 3-4 , November 1959, pp. 594 - 600
Copyright
Copyright © Cambridge University Press 1959

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References

REFERENCES

Baar, H. S. (1957). Discussion in the symposium on toxoplasmosis. Trans. R. Soc. Trop. Med. Hyg. 51, 120–1.Google Scholar
Beverley, J. K. A. & Beattie, C. P. (1952). Standardisation of the dye test for toxoplasmosis. J. Clin. Path. 5, 350–3.CrossRefGoogle ScholarPubMed
Bringmann, G. & Holz, J. (1953 a). Toxoplasma gondii im elektronenmikroskopischen Bild. Z. Hyg. InfektKr. 137, 186–91.CrossRefGoogle Scholar
Bringmann, G. & Holz, J. (1953 b). Licht- und elektronen-mikroskopische Untersuchungen zum Sero-Farbtest auf Toxoplasmose nach Sabin und Feldman. Z. Hyg. InfektKr. 138, 151–4.CrossRefGoogle Scholar
Cathie, I. A. B. & Dudgeon, J. A. (1953). The interpretations of tests for toxoplasmosis. Great Ormond Street Journal, 5, 13–18.Google Scholar
Cross, J. B. (1947). A cytologic study of Toxoplasma with special reference to its effect on the host's cell. J. Infect. Dis. 80, 278–96.CrossRefGoogle ScholarPubMed
Dasgupta, B. (1958). Basophilia in the extracellular form of Toxoplasma gondii (Demonstration). Trans. R. Soc. Trop. Med. Hyg. 52, 297.Google Scholar
Frenkel, J. K. (1953). Host, strain and treatment variation as factors in the pathogenesis of toxoplasmosis. Amer. J. Trop. Med. 2, 392–415.Google ScholarPubMed
Frenkel, J. K. (1956). Pathogenesis of toxoplasmosis and of infections with organisms resembling Toxoplasma. Ann. N.Y. Acad. Sci. 64, 215–31.CrossRefGoogle Scholar
Frenkel, J. K. & Friedlander, S. (1951). Toxoplasmosis: Pathology of neonatal disease, pathogenesis, diagnosis and treatment. U.S. Public Health Service Publication, no. 141.Google Scholar
Grönroos, P. (1955). Studies on Toxoplasma and the serology of toxoplasmosis. Ann. Med. exp. Fenn. 33, suppl. 11, 1–113.Google ScholarPubMed
Holz, J. (1954). Über Toxoplasma gondii und seine Affinität zu den Geweben des Wirtes. Acta trop. Basel, 11, 354–78.Google Scholar
Holz, J. & Bringmann, G. (1954). Vergleichende elektronen-mikroskopische Untersuchungen an Toxoplasmen und Sarcoporidien. Z. Hyg. InfektKr. 139, 239–42.CrossRefGoogle Scholar
Lillie, R. D. (1947). Reactions of various parasitic organisms in tissues to the Bauer, Feulgen, Gram and Gram-Weigert methods. J. Lab. Clin. Med. 32, 76–88.Google Scholar
Sassuchin, D. (1933). K izucheniyu yaderny¯kh substantsiiˇ prosteiiˇshikh i bakteriiˇ. Rev. Microbiol. Saratov, 12, 277–84.Google Scholar
Sassuchin, D. (1935). Zum Studium der Protisten- und Bakterienkerne. Mitteilung I. Über die Nuclealreaktion und ihre Anwendung bei protozoologischen und bakteriologischen Untersuchungen. Arch. Protistenk. 84, 186–98.Google Scholar
Varela, G., Roch, E. & Vázquez, A. (1955). Virulencia, cultivo, polisacaridos, toxinas y la prueba del colorante, estudiados con cepa de Toxoplasma gondii. Rev. Inst. Salubr. Enferm. trop., Méx., 15, 73–80.Google ScholarPubMed