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Spin-labelled nucleic acids

Published online by Cambridge University Press:  17 March 2009

S. G. Kamzolova and
G. B. Postnikova
S. G. Kamzolova
Affiliation:
Institute of Biological Physics, Pushchino, Moscow Region, USSR
G. B. Postnikova
Affiliation:
Institute of Biological Physics, Pushchino, Moscow Region, USSR
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Extract

The spin label method developed by McConnell 15 years ago is now widely used in studies of the structure and dynamic properties of a variety of the biological systems such as proteins and protein complexes, lipids and membranes, nucleic acids, nucleoproteins, etc.

The ESR spectrum of the nitroxide radcal – the spin label – is very sensitive to its microenvironment and permits easy registration of even subtle alterations in it. If spin labels are attached to different sites of a macromolecule the information can be gained about conformational properties of all these local regions and, as a result, about the dynamic behaviour of the object as a whole.

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Research Article
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Quarterly Reviews of Biophysics , Volume 14 , Issue 2 , May 1981, pp. 223 - 288
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Copyright © Cambridge University Press 1981

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References

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