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Why do stored hydrated recalcitrant seeds die?

Published online by Cambridge University Press:  19 September 2008

N.W. Pammenter,
P. Berjak,
J.M. Farrant,
M.T. Smith and
G. Ross
N.W. Pammenter*
Affiliation:
Department of Biology, University of Natal, King George V Ave, Durban, 4001 South Africa
P. Berjak
Affiliation:
Department of Biology, University of Natal, King George V Ave, Durban, 4001 South Africa
J.M. Farrant
Affiliation:
Department of Botany, University of Cape Town, Private Bag, Rondebosch, 7700 South Africa
M.T. Smith
Affiliation:
Department of Botany, University of Natal, PO Box 375, Pietermaritzburg 3600 South Africa
G. Ross
Affiliation:
Department of Chemistry and Applied Chemistry, University of Natal, King George V Ave, Durban, 4001 South Africa
*
* Correspondence
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Abstract

A characteristic of recalcitrant seeds is that, if they are maintained under storage conditions that prevent water loss, they will ultimately lose viability. A current view is that hydrated recalcitrant seeds are metabolically active and undergo germination-associated changes in storage. Some of these changes, such as extensive vacuolation and increase in cell size, imply a requirement for water additional to that present in the seed on shedding. It is therefore suggested that, in storage, recalcitrant seeds are exposed to an initially mild, but increasingly severe, water stress. Deleterious events associated with a water stress of considerable duration are suggested to lead ultimately to the death of the tissue.

The damage that occurs on prolonged storage is unlikely to be associated with an inability to form glasses or prevent membrane lipid phase changes, as absolute water contents are higher than those at which these mechanisms become important. It is considered that the most likely process leading to death of water-stressed (as opposed to dehydrated) tissue is a breakdown of co-ordination of metabolism, leading to uncontrolled free-radical-mediated oxidative damage.

It is generally difficult to maintain tissue in a mild water-stressed condition for extended periods. Stored, hydrated, recalcitrant seeds may provide an ideal model system for studying the metabolic effects of prolonged mild water stress.

Keywords

desiccation tolerance/sensitivityrecalcitranceseed storageseed viabilityseed ageingwater stress

Information

Type
Short Communication
Information
Seed Science Research , Volume 4 , Issue 2 , June 1994, pp. 187 - 191
Copyright
Copyright © Cambridge University Press 1994

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References

Berjak, P., Dini, M. and Pammenter, N.W. (1984) Possible mechanisms underlying the differing dehydration responses in recalcitrant and orthodox seeds: desiccation-associated subcellular changes in propagules of Avicennia marina. Seed Science and Technology 12, 365–384.Google Scholar
Berjak, P., Pammenter, N.W. and Vertucci, C.W. (1992) Homoiohydrous (recalcitrant) seeds: developmental status, desiccation sensitivity and the state of water in the axes of Landolphia kirkii Dyer. Planta 186, 249–261.CrossRefGoogle ScholarPubMed
Berjak, P., Vertucci, C.W. and Pammenter, N.W. (1993) Effects of developmental status and dehydration rate on characteristics of water and desiccation-sensitivity in recalcitrant seeds of Camellia sinensis. Seed Science Research 3, 155–166.CrossRefGoogle Scholar
Bewley, J.D. (1979) Physiological aspects of desiccation tolerance. Annual Review of Plant Physiology 30, 195–238.CrossRefGoogle Scholar
Chin, H.F. and Roberts, E.H. (1980) Recalcitrant crop seeds. Kuala Lumpur, Tropical Press SDN.BDH.Google Scholar
Espindola, L.S., Corbineau, F. and Côme, D. (1993) Early events occurring during dehydration of recalcitrant embryos of Araucaria angustifolia seeds. pp 873–878 in Côme, D., and Corbineau, F. (Eds) Fourth international workshop on seeds: basic and applied aspects of seed biology. Paris, ASFIS.Google Scholar
Farrant, J.M., Berjak, P. and Pammenter, N.W. (1985) The effect of drying rate on viability retention of recalcitrant propagules of Avicennia marina. South African Journal of Botany 51, 432–438.CrossRefGoogle Scholar
Farrant, J.M., Pammenter, N.W. and Berjak, P. (1986) The increasing desiccation sensitivity of recalcitrant Avicennia marina seeds with storage time. Physiologia Plantarum 67, 291–298.CrossRefGoogle Scholar
Farrant, J.M., Pammenter, N.W. and Berjak, P. (1989) Germination associated events and the desiccation sensitivity of recalcitrant seeds: a study on three unrelated species. Planta 178, 189–198.CrossRefGoogle Scholar
Finch-Savage, W.E. (1992) Embryo water status and survival in the recalcitrant species Quercus robur L.: evidence for a critical moisture content. Journal of Experimental Botany 43, 663–669.CrossRefGoogle Scholar
Finch-Savage, W.E., Grange, R.I., Hendry, G.A.F. and Atherton, N.M. (1993) Embryo water status and loss of viability during desiccation in the recalcitrant species Quercus robur L. pp 723–730 inCôme, D. Côme, D. and Corbineau, F. (Eds) Fourth international workshop on seeds: basic and applied aspects of seed biology. Paris, ASFIS.Google Scholar
Flower, D.J. and Ludlow, M.M. (1986) Contribution of osmotic adjustment to the dehydration tolerance of water-stressed pigeon pea (Cajanus cajan (L.) Millsp.) leaves. Plant Cell and Environment 9, 33–40.Google Scholar
Hendry, G.A.F. (1993) Oxygen free radical processes and seed longevity. Seed Science Research 3, 141–153.CrossRefGoogle Scholar
Hendry, G.A.F., Finch-Savage, W.E., Thorpe, P.C., Atherton, N.M., Buckland, S.M., Nilsson, K.A. and Seel, W.E. (1992) Free radical processes and loss of seed viability during desiccation in the recalcitrant species Quercus robur L. New Phytologist 122, 273–279.CrossRefGoogle ScholarPubMed
Hrazdina, G. and Jensen, R.A. (1992) Spatial organization of enzymes in plant metabolic pathways. Annual Review of Plant Physiology and Plant Molecular Biology 43, 241–267.CrossRefGoogle Scholar
Hsiao, T.C. (1973) Plant responses to water stress. Annual Review of Plant Physiology 24, 519–570.CrossRefGoogle Scholar
Kermode, A.R. (1990) Regulatory mechanisms involved in the transition from seed development to germination. Critical Reviews in Plant Sciences 9, 155–195.CrossRefGoogle Scholar
Leopold, A.C.(Ed.) (1986) Membranes, metabolism and dry organisms. Ithaca, New York, Cornell University Press.Google Scholar
Leprince, O., Deltour, R., Thorpe, P.C., Atherton, N.M. and Hendry, G.A.F. (1990) The role of free radicals and radical processing systems in loss of desiccation tolerance in germinating maize (Zea mays L.). New Phytologist 116, 573–580.CrossRefGoogle Scholar
Leprince, O., Hendry, G.A.F. and McKersie, B.D. (1993) The mechanisms of desiccation tolerance in developing seeds. Seed Science Research 3, 231–246.CrossRefGoogle Scholar
Levitt, J. (1980) Responses of plants to environmental stresses. Vol II. Water, radiation, salt and other stresses. New York, Academic Press.Google Scholar
Pammenter, N.W., Vertucci, C.W. and Berjak, P. (1991) Homeohydrous (recalcitrant) seeds: dehydration, the state of water and viability characteristics in Landolphia kirkii. Plant Physiology 96, 1093–1098.CrossRefGoogle ScholarPubMed
Pammenter, N.W., Vertucci, C.W. and Berjak, P. (1993) Responses to desiccation in relation to non-freezable water in desiccation-sensitive and -tolerant seeds. pp 867–872 in Côme, D. and Corbineau, F. (Eds) Fourth international workshop on seeds: basic and applied aspects of seed biology. Paris, ASFIS.Google Scholar
Rubinstein, B., Mahar, P. and Tatter, T.A. (1977) Effects of osmotic shock on some membrane-regulated events of oat coleoptile cells. Plant Physiology 59, 365–368.CrossRefGoogle ScholarPubMed
Smith, M.T. and Berjak, P. (in press) Deteriorative changes associated with the loss of viability of stored desiccation-tolerant and -sensitive seeds. in Ngebi, M. and Kigel, J. (Eds) Seed development and germination: applied aspects. New York, Marcel Dekker.Google Scholar
Tompsett, P.B. and Pritchard, H.W. (1993) Water status changes during development in relation to the germination and desiccation tolerance of Aesculus hippo-castanum seeds. Annals of Botany 71, 107–116.CrossRefGoogle Scholar
Vertucci, C.W. (1990) Calorimetric studies of the state of water in seed tissues. Biophysical Journal 58, 1463–1471.CrossRefGoogle ScholarPubMed
Vertucci, C.W. (1993) Towards a unified hypothesis of seed aging. pp 739–746 in Côme, D. and Corbineau, F. (Eds) Fourth international workshop on seeds: basic and applied aspects of seed biology. Paris, ASFIS.Google Scholar
Vertucci, C.W. and Farrant, J.M. (in press) Acquisition and loss of desiccation tolerance. in Ngebi, M. and Kigel, J. (Eds) Seed development and germination: applied aspects. New York, Marcel Dekker.Google Scholar
Villiers, T.A. (1973) Ageing and longevity of seeds in the field. pp 265–288 in Heydecker, W. (Ed.) Seed ecology. London, Butterworth.Google Scholar