Hostname: page-component-7b7567c8c-fvjsw Total loading time: 0 Render date: 2026-11-12T18:56:34.852Z Has data issue: false hasContentIssue false
  • English
  • Français

EPTC Induced Modification of Gibberellin Biosynthesis

Published online by Cambridge University Press:  12 June 2017

R. E. Wilkinson and
D. Ashley
R. E. Wilkinson
Affiliation:
Dep. Agron., Univ. of Georgia, Georgia Station, Experiment, GA 30212 and Athens, GA 30602
D. Ashley
Affiliation:
Dep. Agron., Univ. of Georgia, Georgia Station, Experiment, GA 30212 and Athens, GA 30602
Get accessRights & Permissions [Opens in a new window]

Abstract

Wheat (Triticum aestivum L. ‘Holley’) was grown for 14 days in 0.5 strength Hoagland and Arnon's complete mineral nutrient solution containing 0. 15.6, 31.25, 62.5, 125.0, or 250.0 ppbw EPTC (S-ethyl dipropylthiocarbamate). Gibberellic acid (GA) content, as measured by gas-liquid chromatography, was decreased 96.4% by 125.0 ppbw EPTC. When 0.05 μCi mevalonic acid-2-14C-(MVA-2-14C) (DBED salt) (0.15 mCi/mg MVA) was added to the nutrient solution, incorporation of MVA-2-14C into total kaurenoids was decreased 90% by 250 ppbw EPTC. Metabolism of kaurene was reduced by 250 ppbw EPTC with resultant 3-fold accumulations of kaurene.

Keywords

Kaurenekaurene synthetasekaurene oxidase

Information

Type
Research Article
Information
Weed Science , Volume 27 , Issue 3 , May 1979, pp. 270 - 274
Copyright
Copyright © 1979 by the Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

Literature Cited

1. Das, V. S. Rama and Santakumori, M. 1975. Stomatal behavior towards four classes of herbicides as a basis of selectivity to certain weeds and crop plants. Proc. Indian Acad. Sci. Sect., B. 82:108–116.Google Scholar
2. Davis, L. A., Heinz, D. E., and Addicott, F. T. 1968. Gas-lqiuid chromatography of trimethylsilyl derivatives of abscisic acid and other plant hormones. Plant Physiol. 43:1389–1394.Google Scholar
3. Dennis, D. T. and West, C. A. 1967. Biosynthesis of gibberellins. III. The conversion of (-)-kaurene to (-)-kaurene-19-oic acid in endosperm of Echinocystis macrocarpa Greene. J. Biol. Chem. 242:3293–3300.Google Scholar
4. Dennis, D. T., Upper, C. D., and West, C. A. 1965. An enzymic site of inhibition of gibberellin biosynthesis by Amo 1618 and other plant growth retardants. Plant Physiol. 40:948–952.Google Scholar
5. Donald, W. W., Harvey, R. G., and Fawcett, R. S. 1977. The role of gibberellins in the abnormal morphogenesis of EPTC injured corn seedlings. Weed Sci. Soc. Am. Abstr. p. 92.Google Scholar
6. Frost, R. G. and West, C. A. 1977. Properties of kaurene synthetase from Marah macrocarpus . Plant Physiol. 59:22–29.Google Scholar
7. Goodwin, T. W. 1965. Regulation of terpenoid synthesis in higher plants. Pages 57–71. in Pridham, J. B. and Swain, T., eds. Biosynthetic pathways in higher plants. Academic Press, New York.Google Scholar
8. Graebe, J. E., Dennis, D. T., Upper, C. D., and West, C. A. 1965. Biosynthesis of gibberellins. I. The biosynthesis of (-)-kaurene, (-)-kauren-10-ol, and trans-geranylgeraniol in endosperm nucellus of Echinocystis macrocarpa Greene. J. Biol. Chem. 240:1847–1854.Google Scholar
9. Hasson, E. P. and West, C. A. 1976. Properties of the system for the mixed function oxidation of kaurene and kaurene derivatives in microsomes of the immature seed of Marah macrocarpus. Cofactor requirements. Plant Physiol. 58:473–478.Google Scholar
10. Hasson, E. P. and West, C. A. 1976. Properties of the system for the mixed function oxidation of kaurene and kaurene derivatives in microsomes of the immature seed of Marah macrocarpus. Electron transfer components. Plant Physiol. 58:479–484.Google Scholar
11. Hoagland, D. R. and Arnon, D. I. 1950. The water-culture method for growing plants without soil. California Agric. Exp. Stn. Circular 347. 32 pp.Google Scholar
12. Karunen, P. and Eronen, L. 1977. Influence of S-ethyl dipropylthiocarbamate (EPTC) on the fatty acid composition of wheat leaf galactolipids. Physiol. Plant 40:101–104.Google Scholar
13. Karunen, P., Valanne, N., and Wilkinson, R. E. 1976. Influence of S-ethyl dipropylthiocarbamate on growth, chlorophyll and carotenoid production and chloroplast ultrastructure of germinating Polytrichum commune spores. Bryologist 79:332–338.Google Scholar
14. Karunen, P. and Wilkinson, R. E. 1975. Influence of S-ethyl dipropylthiocarbamate (EPTC) on wheat root phospholipid fatty acid composition. Physiol. Plant 35:228–231.Google Scholar
15. Kontz, J., Coolbaugh, R. C., and West, C. A. 1977. Regulation of the biosynthesis of ent-kaurene from mevalonate in the endosperm of immature Marah macrocarpus seeds by adenylate energy charge. Plant Physiol. 60:81–85.Google Scholar
16. Mahler, H. R. and Cordes, E. H. 1971. Biological Chemistry. 2nd ed. Harper and Row, Publ. New York. 1009 pp.Google Scholar
17. Radice, M. and Cocucci, S. M. 1973. An analysis of the possible mechanism of action of a thiocarbamate herbicide:molinate. Inf. Bot. Ital. 5:112.Google Scholar
18. Seifermann, D. and Yamamoto, H. Y. 1974. Light-induced deepoxidation of violaxanthin in lettuce chloroplasts. III. Reaction kinetics and effect of light intensity on de-epoxidase activity and substrate availability. Biochim. Biophys. Acta 357:144–150.Google Scholar
19. Wilkinson, R. E. 1977. Zeaxanthin epoxidation inhibition by EPTC. Bot. Gaz. 138:270–275.Google Scholar
20. Wilkinson, R. E. 1978. Quinone accumulation in S-ethyl dipropylthiocarbamate (EPTC) treated wheat. Pestic. Biochem. Physiol. 8:208–214.Google Scholar
21. Wilkinson, R. E. and Karunen, P. 1976. Influence of S-ethyl dipropylthiocarbamate on atrazine absorption by wheat. Ann. Bot. 40:1043–1046.CrossRefGoogle Scholar
22. Wilkinson, R. E. and Karunen, P. 1977. Water utilization by S-ethyl dipropylthiocarbamate treated wheat. Weed Res. 17:335–338.Google Scholar
23. Wilkinson, R. E., Michel, B., and Smith, A. E. 1977. Alteration of soybean complex lipid biosynthesis by S-ethyl dipropylthiocarbamate (EPTC). Plant Phsyiol. 60:86–88.Google Scholar