Hostname: page-component-7b7567c8c-fvjsw Total loading time: 0 Render date: 2026-11-14T20:18:40.185Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunoassay to Detect Enhanced Carbamothioate Degradation in Soil

Published online by Cambridge University Press:  12 June 2017

Pamela J. Hutchinson,
Patrick J. Shea,
James M. Takacs and
Paul E. Staswick
Pamela J. Hutchinson
Affiliation:
Dep. Agron., Chem. Dep., and Dep. Agron., Univ. Nebr., Lincoln, NE 68583-0915
Patrick J. Shea
Affiliation:
Dep. Agron., Chem. Dep., and Dep. Agron., Univ. Nebr., Lincoln, NE 68583-0915
James M. Takacs
Affiliation:
Dep. Agron., Chem. Dep., and Dep. Agron., Univ. Nebr., Lincoln, NE 68583-0915
Paul E. Staswick
Affiliation:
Dep. Agron., Chem. Dep., and Dep. Agron., Univ. Nebr., Lincoln, NE 68583-0915
Get accessRights & Permissions [Opens in a new window]

Abstract

An indirect enzyme-linked immunosorbent assay (ELISA) for EPTC was developed that may be useful for predicting enhanced carbamothioate herbicide degradation in adapted soils. A modified radioimmunoassay (RIA) procedure was used to detect antibodies specific for EPTC and establish antibody dilutions for optimum ELISA sensitivity. Antibodies were obtained from a rabbit injected with a butylate-analog protein conjugate. An indirect ELISA subsequently was developed for EPTC with a detection range of 10 to 1280 ng ml−1 in a 1:3 aqueous dilution of a 90:10 (v/v) acetonitrile (ACN):water soil extract. EPTC recovery from soil was > 96%, as determined by conventional gas chromatography. Measurement of low concentrations of residual EPTC with the ELISA was less quantitative than by gas chromatography, but the immunoassay provided a rapid, potentially cost-effective method for screening soils to determine enhanced carbamothioate degradation.

Keywords

Butylate, S-ethyl bis(2-methylpropyl)carbamothioateEPTC, S-ethyl dipropyl carbamothioateEnzyme-linked immunosorbent assayELISAradioimmunoassayRIAenhanced degradation

Information

Type
Research
Information
Weed Technology , Volume 7 , Issue 2 , June 1993, pp. 396 - 403
Copyright
Copyright © 1993 Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

Literature Cited

1. Bushway, R. J., Perkins, B., Savage, S. A., Lekouski, S. J., and Ferguson, B. S. 1988. Determination of atrazine residues in water and soil by enzyme immunoassay. Bull. Environ. Contam. Toxicol. 40:647–654.CrossRefGoogle ScholarPubMed
2. Deschamps, R.J.A., Hall, J. C., and McDermott, M. R. 1990. Polyclonal and monoclonal enzyme immunoassays for picloram detection in water, soil, plants and urine. J. Agric. Food Chem. 38:1881–1886.CrossRefGoogle Scholar
3. Dreher, R. M. and Podratzki, B. 1988. Development of an immunoassay for endosulfan and its degradation products. J. Agric. Food Chem. 36:1072–1075.CrossRefGoogle Scholar
4. Garen, A. and Levinthal, C. 1960. A fine-structured genetic and chemical study of the enzyme alkaline phosphatase from E. coli. I. Purification and characterization of alkaline phosphatase. Biochim. Biophys. Acta 38:470.CrossRefGoogle Scholar
5. Gee, S. J., Miyamoto, T., Goodrow, M. H., Buster, D., and Hammock, B. D. 1988. Development of an enzyme-linked immunosorbent assay for the analysis of the thiocarbamate herbicide molinate. J. Agric. Food Chem. 36:863–870.Google Scholar
6. Goolsby, D. A., Thurman, E. M., Clark, M. L., and Pomes, M. L. 1991. Immunoassay as a screening tool for triazine herbicides in streams. p. 86–99 in Vanderlaan, M., Stahnker, L. H., Watkins, B. E., and Roberts, D. W., eds. Immunoassays for Trace Chemical Analysis. Am. Chem. Soc. Symp. Ser. 451, Am. Chem. Soc., Washington, DC.Google Scholar
7. Hall, J. C., Deschamps, R.J.A., and Krieg, K. K. 1989. Immunoassays for the detection of 2,4-D and picloram in river water and urine. J. Agric. Food Chem. 37:981–984.Google Scholar
8. Hall, J. C., Deschamps, R.J.A., and McDermott, M. R. 1990. Immunoassays to detect and quantitate herbicides in the environment. Weed Technol. 4:226–234.Google Scholar
9. Hammock, B. D. and Mumma, R. O. 1980. Potential of immunochemical technology for pesticide analysis. p. 321–352 in Harvey, J. Jr. and Zweig, G., eds. Pesticide Analytical Methodology. American Chemical Society, Washington, DC.CrossRefGoogle Scholar
10. Harrison, R. O., Goodrow, M. H., and Hammock, B. D. 1991. Competitive inhibition ELISA for the s-triazine herbicides: Assay optimization and antibody characterization. J. Agric. Food Chem. 39:122–128.Google Scholar
11. Herbert, W. J. 1978. Passive hemagglutination. p. 1–20 in Weir, D. M., ed. Handbook of Experimental Immunology, Vol. 1. Blackwell Scientific Press, Oxford.Google Scholar
12. Hutchinson, P. J. 1991. Immunoassay for carbamothioate detection and application to enhanced pesticide degradation. Ph.D. Dissertation, Univ. Nebr., Lincoln. 79 p.Google Scholar
13. Jung, F., Gee, S. J., Harrison, R. O., Goodrow, M. H., Kara, A. E., Bruan, A. L., Li, Q. X., and Hammock, B. D. 1989. Use of immunochemical techniques for the analysis of pesticides. Pestic. Sci. 26:303–317.Google Scholar
14. Jung, F., Meyer, H.H.D., and Hamm, R. T. 1989. Development of a sensitive enzyme-linked immunosorbent assay for the fungicide fenpropimorph. J. Agric. Food Chem. 37:1183–1187.Google Scholar
15. Kelley, M. M., Zahnow, E. W., Petersen, W. C., and Toy, S. T. 1985. Chlorsulfuron determination by soil extracts by enzyme immunoassay. J. Agric. Food Chem. 33:962–965.Google Scholar
16. Koppatschek, F. K., Liebl, R. A., Kriz, A. L., and Melhado, L. L. 1990. Development of an enzyme-linked immunosorbent assay for the detection of the herbicide clomazone. J. Agric. Food Chem. 38:1519–1522.CrossRefGoogle Scholar
17. Linn, D. M. and Doran, J. W. 1984. Effect of water-filled pore space on carbon dioxide and nitrous oxide production in tilled and nontilled soils. Soil Sci. Soc. Am. J. 48:1267–1272.Google Scholar
18. Petersen, B. B. 1990. Interactions between agricultural chemicals and their effects on pesticide persistence in soil. Ph.D. dissertation, Univ. Nebr., Lincoln. 114 p.Google Scholar
19. Riggle, B. and Dunbar, B. 1990. Development of enzyme immunoassay for the detection of the herbicide norflurazon. J. Agric. Food Chem. 38:1922–1925.CrossRefGoogle Scholar
20. Schlaeppi, J.-M., Meyer, W., and Ramsteiner, K. A. 1992. Determination of triasulfuron in soil by monoclonal antibody-based enzyme immunoassay. J. Agric. Food Chem. 40:1093–1098.CrossRefGoogle Scholar
21. Schlaeppi, J.-M., Moser, H., and Ramsteiner, K. A. 1991. Determination of metolachlor by competitive enzyme immunoassay using a specific monoclonal antibody. J. Agric. Food Chem. 39:1533–1536.CrossRefGoogle Scholar
22. Schwalbe, M., Dorn, E., and Beyermann, K. 1984. Enzyme immunoassay and fluoroimmunoassay for the herbicide diclofop-methyl. J. Agric. Food Chem. 32:734–741.Google Scholar
23. Wie, S. I. and Hammock, B. D. 1984. Comparison of coating and immunizing antigen structure on the sensitivity and specificity of immunoassays for benzoylphenylurea insecticides. J. Agric. Food Chem. 32:1294–1301.Google Scholar