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Published online by Cambridge University Press: 12 June 2017
The method was based on the minimum dosage of 2,4–dichlorophenoxyacetic acid (2,4–D) that would produce strapping or cupping of cotton leaves. Dilutions of an unknown were applied to cotton cotyledons. The minimum dosage required to produce strapped or cupped leaves two weeks later was approximately 0.05 μg/plant. The results of this bioassay were comparable to tracer methods, but the latter can detect smaller quantities of 2,4–D.