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Cell surface localisation and stability of uvomorulin during early mouse development

Published online by Cambridge University Press:  26 September 2008

Lesley Clayton,
Siân V. Stinchcombe and
Martin H. Johnson
Lesley Clayton*
Affiliation:
Department of Anatomy, University of Cambridge, Cambridge, UK.
Siân V. Stinchcombe
Affiliation:
Department of Anatomy, University of Cambridge, Cambridge, UK.
Martin H. Johnson
Affiliation:
Department of Anatomy, University of Cambridge, Cambridge, UK.
*
Dr L. Clayton, Department of Anatomy, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK. Tel: 0223 333755. Fax: 0223 333786.
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Extract

We have examined immunocytochemically the subcellular distribution of the cell adhesion molecule uvomorulin in cleavage stage mouse embryos using conventional and confocal microscopy, under a range of detergent extraction and fixation regimes. Only traces of uvomorulin were detectable on the surface of unfertilised oocytes, whereas between 6 and 11 h after activation detergent-resistant surface expression was evident. This shift correlates with previously demonstrated changes in the pattern of synthesis and accumulation of uvomorulin from precursor state in unfertilised oocytes to mature protein after fertilisation. Embryos at subsequent stages up to the 8-cell stage exhibited a uniform distribution of uvomorulin on free surfaces and its concentration in regions of contact between blastomeres. At the 8-cell stage, during compaction, there was increased intercellular adhesion with concomitant accumulation of uvomorulin at intercellular contacts, whilst free surface uvomorulin was reduced and became relatively more susceptible to detergent extraction. When compact 8-cell embryos were decompacted in calcium-free medium, uvomorulin at contacts decreased while free surface and cytoplasmic staining increased. Blastomeres disaggregated from 4- and 8-cell embryos showed traces or ‘footprints’ of anti-uvomorulin staining in regions previously in apposition. These footprints disappeared over 45–60 min, during which time uvomorulin distribution became uniform. Possible mechanisms underlying the rearrangements which take place both at fertilisation and during compaction and experimental decompaction are discussed.

Keywords

CompactionEmbryoMouseOocyteUvomorulin

Information

Type
Article
Information
Zygote , Volume 1 , Issue 4 , November 1993, pp. 333 - 344
Copyright
Copyright © Cambridge University Press 1993

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