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Molecular structure and function of CD4 on murine egg plasma membrane

Published online by Cambridge University Press:  26 September 2008

Mao Wu Guo,
Toshiki Watanabe,
Etsuko Mori and
Tsuneatsu Mori
Mao Wu Guo
Affiliation:
Department of Immunology and Pathology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
Toshiki Watanabe
Affiliation:
Department of Immunology and Pathology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
Etsuko Mori
Affiliation:
Department of Immunology and Pathology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
Tsuneatsu Mori*
Affiliation:
Department of Immunology and Pathology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
*
T.Mori, Department of Immunology, Institute of Medical science, University of Tokyo, 4-6-1, Shirokanedai Minatoku, Tokyo 108, Japan. Telephone: +81-3-5449-5261. Fax: +8-3-3443-3893.
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Summary

In the present study, the expression of the CD4 molecule on murine egg plasma membrane was confirmed by the indirect immunofluorescence (IIF) method. The full-length CD4 cDNA from murine eggs was synthesised by the reverse transcriptase-polymerase chain reaction (RT-PCR) method and its authenticity verified by Southern blot hybridisation using an end-labelled internal oligonucleotide. The results of DNA sequencing showed that the nucleotide sequence of the cDNA of CD4 from murine egg mRNA was identical to that of immune T cells. To demonstrate the direct interaction of CD4 from murine egg with murine sperm cells bearing MHC (major histocompatibility complex) class II molecule, we employed a baculovirus expression system to generate CD4 on the surface of Spodoptera frugiperda (Sf9) cells. Expression of CD4 on Sf9 cells infected with autographa californica nuclear polyhedrosis virus (AcNPV)-CD4 was demonstrated by IIF and immunoblotting. The CD4-expressing Sf9 cells adhered to MHC class II-bearing sperm cells since the adhension was specifically blocked by anti-CD4 monoclonal antibody (mAb) or anti-monomorphic region of MHC class II mAb. Taking our previous and present experimental results together, they strongly suggest that intercellular membrane adhesion between two gametes at the fusion step in fertilisation is mediated by the MHC class II molecule located on the posterior region of the sperm head and the CD4 molecule on egg plasma membrane.

Keywords

CD4EggMHC class IISf9Sperm

Information

Type
Article
Information
Zygote , Volume 3 , Issue 1 , February 1995, pp. 65 - 73
Copyright
Copyright © Cambridge University Press 1995

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Footnotes

1Department of Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
1Department of Pathology, Institute of Medical science, University of Tokyo, Tokyo, Japan.

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