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Murine Asb-17 expression during mouse testis development and spermatogenesis

Published online by Cambridge University Press:  09 August 2004

Kye-Seong Kim,
Myung-Sun Kim,
Soo-Kyung Kim and
Kwang-Hyun Baek
Kye-Seong Kim
Affiliation:
Cell and Gene Therapy Research Institute, Graduate School of Life Science and Biotechnology, Pochon CHA University, CHA General Hospital, Seoul, Korea
Myung-Sun Kim
Affiliation:
Cell and Gene Therapy Research Institute, Graduate School of Life Science and Biotechnology, Pochon CHA University, CHA General Hospital, Seoul, Korea
Soo-Kyung Kim
Affiliation:
Cell and Gene Therapy Research Institute, Graduate School of Life Science and Biotechnology, Pochon CHA University, CHA General Hospital, Seoul, Korea
Kwang-Hyun Baek
Affiliation:
Cell and Gene Therapy Research Institute, Graduate School of Life Science and Biotechnology, Pochon CHA University, CHA General Hospital, Seoul, Korea
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Abstract

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In this study we isolated a murine mAsb-17 from mouse testis by RT-PCR using primers designed based on the sequences from the GenBank database. The sequence analysis showed that mAsb-17 encodes a 295 amino acid polypeptide with a molecular weight of approximately 34 kDa containing two ankyrin repeats and one SOCS box. The amino acid sequence of mASB-17 showed 87.5%, 98.3% and 92.9% identity with that of human, rat and dog, respectively. Interestingly, northern blot analysis showed that mAsb-17 was expressed only in the testis. The expression analysis by RT-PCR for mAsb-17 in mouse indicates that mAsb-17 is expressed from the fourth week after birth to adult, with the highest expression in round spermatids. Both northern blot and RT-PCR analyses suggest that mASB-17 may play essential roles in testis development and spermatogenesis.

Keywords

Ankyrin repeatAsb-17SOCS boxSpermatogenesis

Information

Type
Research Article
Information
Zygote , Volume 12 , Issue 2 , May 2004, pp. 151 - 156
Copyright
2004 Cambridge University Press