Introduction
Candelariella Müll. Arg., a cosmopolitan genus of c. 75 species, includes lichenized taxa on all substrata, as well as non-lichenized fungi and lichenicolous parasites. The taxa are predominately nitrophilous with most occurring on rock, bark and plant debris in various undisturbed habitats as well as urban areas (e.g. Hakulinen Reference Hakulinen1954; Westberg Reference Westberg, Nash, Ryan, Diederich, Gries and Bungartz2004, Reference Westberg2005, Reference Westberg2007a, Reference Westbergb, Reference Westbergc; Khodosovtsev Reference Khodosovtsev2005; Westberg et al. Reference Westberg, Frödén and Wedin2009, Reference Westberg, Morse and Wedin2011; Westberg & Sohrabi Reference Westberg and Sohrabi2012; Ismailov et al. Reference Ismailov, Urbanavichus, Yakovchenko and Urbanavichene2017). It is the largest genus of Candelariaceae, introduced by Hakulinen (Reference Hakulinen1954) with three genera, namely Candelaria A. Massal. (as the type), Candelariella and Placomaronea Räsänen. The family currently includes two additional genera, Candelina Poelt (Poelt Reference Poelt1974) and the recently described Protocandelariella Poelt et al. (Kondratyuk et al. Reference Kondratyuk, Lőkös, Jeong, Oh, Kondratiuk and Hur2020) which was tentatively proposed by Poelt (Reference Poelt1974) and subsequently confirmed by molecular studies (Westberg et al. Reference Westberg, Arup and Kärnefelt2007; Yakovchenko et al. Reference Yakovchenko, Vondrák, Ohmura, Korchikov, Vondrákova and Davydov2017; Kondratyuk et al. Reference Kondratyuk, Lőkös, Jeong, Oh, Kondratiuk and Hur2020).
The phylogeny of Candelariella still has not yet been resolved. Miadlikowska et al. (Reference Miadlikowska, Kauff, Hofstetter, Fraker, Grube, Hafellner, Reeb, Hodkinson, Kukwa and Lücking2006) considered that the generic circumscription in Candelariaceae based on the outer morphology (i.e. growth form) only partly reflects the phylogeny. The first comprehensive molecular phylogenetic study of Candelariella (Westberg et al. Reference Westberg, Arup and Kärnefelt2007) based on ITS nrDNA demonstrated that the overall topology of the phylogram was poorly resolved and was strongly supported only at terminal clades due to several ‘good species’ with distinct morphology (Westberg Reference Westberg2007a, Reference Westbergb, Reference Westbergc). Subsequent phylogenies based on ITS nrDNA also confirmed the polyphyletic nature of Candelariella and Candelaria and were not well-resolved; however, they were still useful at a specific level and several new species were described (Westberg et al. Reference Westberg, Morse and Wedin2011; Yakovchenko et al. Reference Yakovchenko, Vondrák, Ohmura, Korchikov, Vondrákova and Davydov2017; Liu et al. Reference Liu, Wang, Wang and Hur2019; Halıcı et al. Reference Halıcı, Kahraman Yiğit, Bölükbaşi and Güllü2023). The addition of several molecular markers did not give the desired result since the recent phylogeny (Kondratyuk et al. Reference Kondratyuk, Lőkös, Jeong, Oh, Kondratiuk and Hur2020) based on mostly nrITS and a small number of additional sequences of 12S mtSSU and 28S nrLSU demonstrated that the backbone of the phylogram was still poorly supported; nevertheless, three new genera were described. Cannon et al. (Reference Cannon, Coppins, Orange, Sanderson and Simkin2021) suggested that the phylogenetic relationships within non-monophyletic Candelariella need additional research. Protocandelariella is morphologically and phylogenetically distinct whereas the segregation of two other genera, Candelinella S. Y. Kondr. and Opeltiella S. Y. Kondr., requires additional phenotypic and phylogenetic evidence.
The characters thought to be taxonomically important for Candelariella were summarized in Hakulinen (Reference Hakulinen1954), Poelt (Reference Poelt1974) and Westberg (Reference Westberg2005). The main taxonomically important features to define the taxa are: life strategy, thallus growth form, colour and texture of the upper surface, type of apothecia, ascospore size, shape, septation and number per ascus, presence or absence of vegetative propagules and their localization, as well as substratum and ecology.
The group of Candelariella s. lat. with 8-spored asci is the largest among the genus, comprising 41 lichen taxa. It is represented by lichenized or lichenicolous species with all possible variations of thallus morphology, occurring on various substrata in habitats worldwide including both the Arctic and Antarctic. While checking herbarium material from Mongolia collected by the first author, our attention was caught by a Candelariella sp. with a cushion-forming, greenish yellow pulverulent thallus growing not directly on siliceous rock surfaces but in rock crevices and easily falling down from the substratum. Morphologically and ecologically, it resembled the American taxon Candelariella citrina de Lesd., known mostly from North America. Anatomical investigation showed that the specimens had no citriform ascospores which are characteristic of C. citrina. The same material was collected during the authors' joint fieldwork in 2014 in the Altai Mountains and Russian Far East, and also found after checking herbarium material collected by the second author from China. Both morphological and molecular studies have independently confirmed its status as a new species, described here as Candelariella ahtii. A worldwide key to 8-spored Candelariella is also presented.
Materials and Methods
Sampling
Sixteen collections of fresh material of Candelariella ahtii were made by the authors between 2006 and 2014 and are deposited in the private herbarium of Davydov & Yakovchenko, with duplicates deposited in the herbaria ALTB and VLA. The type material was deposited in LE and ALTB as well as being prepared for transfer to H. At each location, material was collected over the sampled boulder or rock, not directly from the rock surface but on soil in cracks and crevices. For each collection, the following were recorded: GPS coordinates, elevation, surrounding environment and substratum. Additionally, specimens of Candelariella citrina, including the type material, were studied from the herbaria H, S, TUR and UPS as well as the private herbaria of B. McCune and J. Hollinger.
Morphology, anatomy and chemistry
Morphological observations were carried out using a stereomicroscope (Zeiss Stemi 2000-C). Apothecia and thalli were hand-sectioned with a razor blade and observed with a compound microscope (Zeiss Axio Lab.A1) after mounting in water. Measurements of the squamules, apothecia, ascospores and thalline margin are presented as the mean (x̄) and range including 85% of the variation, bounded by the smallest and largest observed values and the sample size (n), following Westberg (Reference Westberg2005). Other measurements are presented as (extreme minimum) minimum–maximum (extreme maximum) or the maximum value observed. Measurements were recorded to the nearest 0.5 μm. Lichen substances were studied by spot tests using potassium hydroxide solution (K), sodium hypochlorite solution (C), 1,4-p-phenylendiamine (PD), potassium iodide solution (I), and by thin-layer chromatography (TLC) with solvent systems A, B' and C, following Orange et al. (Reference Orange, James and White2001).
DNA extraction, amplification and sequencing
Between 3–5 apothecia were carefully checked for fungal infections and thoroughly cleaned of extraneous matter. DNA extraction, primers, amplification and sequencing of the internal transcribed spacer region of nuclear ribosomal DNA (ITS) followed the methods of Davydov & Yakovchenko (Reference Davydov and Yakovchenko2017). Cycling conditions included initial denaturation at 94 °C for 5 min, 35 cycles of 95 °C for 20 s, 52 °C for 40 s, 72 °C for 60 s, and a final extension step at 72 °C for 7 min. The program Geneious v. 6.0 (Biomatters Ltd, New Zealand) was used for assembling partial and complementary sequences.
Sequence alignment and phylogenetic analyses
To test the phylogenetic relationship to other species of Candelariella, all obtained sequences of the putative new species were supplemented with sequences of Candelariella species obtained from GenBank (Table 1). Sequences were aligned with those of 35 species, preferably representing type material, from the most comprehensive phylogenetic study of Candelariella by Westberg at al. (Reference Westberg, Arup and Kärnefelt2007) and other recent studies, where possible representing material from Eurasia (Schmull et al. Reference Schmull, Miadlikowska, Pelzer, Stocker-Wörgötter, Hofstetter, Fraker, Hodkinson, Reeb, Kukwa and Lumbsch2011; Westberg et al. Reference Westberg, Morse and Wedin2011; Tripp & Lendemer Reference Tripp and Lendemer2015; Yakovchenko et al. Reference Yakovchenko, Vondrák, Ohmura, Korchikov, Vondrákova and Davydov2017; Liu et al. Reference Liu, Wang, Wang and Hur2019; Urbanavichus et al. Reference Urbanavichus, Vondrák, Urbanavichene, Palice and Malíček2020; Halıcı et al. Reference Halıcı, Kahraman Yiğit, Bölükbaşi and Güllü2023; Vondrák et al. Reference Vondrák, Svoboda, Košnar, Malíček, Šoun, Frolov, Svensson, Novotný and Palice2023). Pycnora xanthococca (Sommerf.) Hafellner was used as the outgroup since Pycnora has been suggested as the sister clade to Candelariaceae (Bendiksby & Timdal Reference Bendiksby and Timdal2013). An ITS1-5.8S rDNA-ITS2 dataset containing the sequences listed in Table 1 was compiled and aligned using the MAFFT algorithm (Katoh et al. Reference Katoh, Rozewicki and Yamada2019) and visible deviations in position homology were then manually optimized. A total of 469 bp alignment sites were used for a heuristic search for the maximum likelihood (ML) bootstrap tree, with simultaneous inference of the optimal partitioning scheme and substitution models (TIM2e + G4 for ITS1, K2P + I for 5.8S, and TPM2u + F + G4 for ITS2 subsets) performed using the online version of IQ-TREE (Nguyen et al. Reference Nguyen, Schmidt, von Haeseler and Minh2015; Trifinopoulos et al. Reference Trifinopoulos, Nguyen, von Haeseler and Minh2016), suggesting three initial partitions (ITS1, 5.8S rDNA, ITS2). Branch lengths were assumed to be equal for all partitions. Branch support was estimated with the ultrafast bootstrap algorithm (Minh et al. Reference Minh, Nguyen and von Haeseler2013) based on 1000 bootstrap replicates and using a maximum of 1000 iterations and a minimum correlation coefficient of 0.99 as a stopping rule.
Voucher information and GenBank Accession numbers for the specimens used in the phylogenetic analyses in this study. Newly generated sequences are shown in bold
Results
The phylogenetic study
To test the monophyly and phylogenetic relationships of the putative new species, we used ITS/5.8S rDNA sequences. ITS sequences were successfully obtained from five specimens from different regions of Asia. Despite long distances between collected specimens, ITS sequences of Candelariella ahtii were identical and combined into a well-supported clade (100% bootstrap support (BS)) (Fig. 1). Candelariella ahtii clusters as sister to C. citrina with high support (97% BS).
Phylogenetic relationships amongst Candelariella species, based on a maximum likelihood analysis of ITS1-5.8S-ITS2. The reliability of each branch was tested by ML analysis. Numbers at tree nodes indicate bootstrap values of ML. Thicker branches indicate a ML bootstrap value of ≥ 70%. GenBank Accession numbers are given in Table 1. Branch lengths represent the estimated number of substitutions per site assuming the respective models of substitution. In colour online.
Candelariella ahtii Yakovchenko sp. nov.
MycoBank No.: MB 854215
Thallus on soil in siliceous rock crevices and cracks, squamulose, up to 1.5(–2.0) mm thick, cushion-forming, split by cracks; of imbricate, large, rounded to weakly incised granules/squamules (0.15–)0.25–0.35–0.5(–0.8) mm diam., constricted at the base into a stipe. Vegetative propagules and prothallus are absent. Upper surface is greenish yellow to pale yellow, pulverulent, lower surface grey. Apothecia lecanorine, rounded to wavy or lobate with a flattened to somewhat convex, ochre-yellow disc, with a permanent, even to incised thalline margin. Asci 8-spored, ascospores ellipsoid to narrowly ellipsoid, rarely broadly ellipsoid, with rounded ends (8.5–)11.0–12.5–14.0(–16.0) × (4.5–)5.0–5.3–5.5(–6.0) μm. Similar to Candelariella citrina but differs by its ascospores with non-attenuated ends. It differs from C. grimmiae Poelt & Reddi by its pulvinate thallus of imbricate large granules/squamules constricted basally into a stipe and its smaller ascospores.
Type: Russia, Altai Territory, Krasnoshchokovsky District, Tigireksky Range, Tigirek Strict Reserve, 7.6 km NW from the Tigirek settlement, Chorny Kamen' Mt, 51°11′29″N, 82°56′52″E, 970 m, conglomerate rocks, on soil and lichens in rock crevices, 20 June 2014, E. A. Davydov 16329 & L. S. Yakovchenko LYF-14-33 (LE-13177—holotype; H, ALTB—isotypes).
(Fig. 2)
A–F, holotype of Candelariella ahtii (LE-13177). A, upper surface showing cushion-forming thallus divided by cracks. B, lower surface, showing grey lower surface constricted into a stipe. C, section of an apothecium, showing compact basal stipe formed by the aurella-type proper margin. D, 8-spored asci. E, ellipsoid to narrowly ellipsoid, or broadly ellipsoid, ascospores with rounded ends. F, section of a squamule. Scales: A = 5 mm; B = 1 mm; C = 50 μm; D & E = 10 μm; F = 100 μm. In colour online.
Thallus squamulose, loosely attached to the substrate, distinct and visible as ±continuous thick cushion-forming crust divided by deep cracks into irregular clusters up to 3.5(–5.0) mm wide and 1.5(–2.0) mm tall (n = 35), composed of imbricate, large granules or subsquamules/squamules, without vegetative propagules. Prothallus absent. Granules/squamules (0.15–)0.25–0.35–0.50(–0.80) mm diam. (n = 155) and up to 0.3 mm tall, convex, imbricate, rounded to weakly incised or rarely sublobate, constricted at the base, into a stipe. Upper surface greenish yellow to pale yellow, pulverulent, coarse, matt. Lower surface grey. Pseudocortex indistinct to thin, of (0)1–2 cell layers, 5.0–12.5 μm thick (n = 25), composed of non-gelatinized hyphae with ±isodiametric, thin-walled cells 3–7 μm wide (n = 25), in uppermost part covered by yellow-brown irregular cortical crystals, without an epinecral layer. Algae chlorococcoid, 5–18 μm diam. (n = 21).
Apothecia common, lecanorine, rounded to wavy or lobate, constricted at the base, crowded to scattered, (0.3–)0.4–0.75–1.2(–1.5) mm wide (n = 105). Disc ochre-yellow, plane to somewhat convex or concave in the centre, brownish tan when old. Proper margin not visible from the outside. Thalline margin of the same colour as the thallus, pulverulent, matt, persistent, at the same level as the disc or lower, entire or slightly incised to sublobate, (0.05–)0.07–0.10–0.15(–0.2) mm thick (n = 55); with a thin pseudocortex, of 1–2(–3) cell layers, 4–18 μm thick (n = 21), thicker at the base of apothecia, composed of thin-walled, ±isodiametric cells, 3–8 μm wide (n = 21), covered by 4–10 μm thick (n = 21) layer of yellow-brown, irregular cortical crystals. Proper margin indistinct to well developed and fan-shaped, 27–40 μm wide (n = 21) in the uppermost part, aurella-type, viz. of branched, thick-walled, radiating hyphae with rectangular cells 5–15 × 2–3 μm (n = 21), which become shorter and rounded in the uppermost part and below the hypothecium form a stipe penetrating downwards through the algal layer. Epihymenium reddish yellow to yellow-brown, 5–12 μm tall (n = 21), of irregular, angular crystals; hymenium hyaline, 50–75 μm tall (n = 21); subhymenium hyaline, with oil droplets; paraphyses septate, simple or branched near the tips, 1.7–3.0 μm wide in mid-hymenium (n = 21) with cylindrical or clavate tips, 3.0–5.5 μm wide (n = 21); asci 8-spored, clavate to broadly clavate, 40–60 × 14–19 μm (n = 21), Candelariella-type; ascospores hyaline, simple, rarely 1-septate with thin septa, straight, ellipsoid to narrowly ellipsoid, or rarely broadly ellipsoid, with rounded ends, (8.5–)11.0–12.5–14.0(–16.0) × (4.5–)5.0–5.3–5.5(–6.0) μm (n = 145).
Pycnidia not seen.
Chemistry
Thallus and disc K+ weakly reddish, KC−, C−, P−; hymenium I+ blue; medulla I−. Calycin, pulvinic acid, pulvinic dilactone and vulpinic acid detected by TLC.
Etymology
The species is named after the great Finnish lichenologist, Teuvo Ahti, dear friend of the authors, with gratitude for his kindness, responsiveness, care and help, as well as with appreciation for his remarkable contribution to the study of Cladonia worldwide.
Ecology
Candelariella ahtii occurs in open siliceous outcrops on boulders or rocks, not directly on the rock surface but on soil in cracks and crevices, sometimes covering saxicolous lichens growing nearby (Fig. 3). It has been recorded at elevations between 390 and 2550 m, but it seems to prefer moderate elevations since most records are found at c. 1000 m a.s.l. In the area of investigation, three vegetation belts are found within this elevational range: forest, montane steppe and alpine. It is mostly recorded in open habitat in alpine or mountain steppe belts. Part of the location is located in the forest belt where the surrounding areas are wooded, mainly Larix sibirica Ledeb. (in Central Asia), Quercus mongolica Fisch. ex Ledeb. or mixed broad-deciduous forest (in the Far East). In these forested habitats, Candelariella ahtii occurs in well-lit open sites, mostly on the upper part of large boulders and rock massifs, but never in very shady areas (Fig. 4).
A & B, field photographs of Candelariella ahtii (LE-13177). A, Candelariella ahtii with neighbouring saxicolous species. B, Candelariella ahtii, in detail. In colour online.
A & B, habitats of Candelariella ahtii. A, type locality in the Altai Mts. B, a local rocky summit with sparse Juniperus rigida Siebold et Zucc. surrounded by Quercus mongolica forest in the Russian Far East. In colour online.
Candelariella ahtii rarely occurs monospecifically, but is often mixed with C. vitellina s. lat. It also occurs with Acarospora badiofusca (Nyl.) Th. Fr., Circinaria sp., Dimelaena oreina (Ach.) Norman, Lecanora achariana A. L. Sm., Montanelia tominii (Oxner) Divakar et al., Rhizocarpon geographicum (L.) DC., R. subgeminatum Eitner, Rhizoplaca chrysoleuca (Sm.) Zopf, R. subdiscrepans (Nyl.) R. Sant. and Rimularia badioatra (Kremp.) Hertel & Rambold.
Distribution
Candelariella ahtii is distributed in Central and North-East Asia. To date this lichen has been collected from several localities in Mongolia, China and Russia (Altai Mountains and Russian Far East). It is known from 43–51°N and 83–133°E with the distance from the most remote localities c. 3800 km (Fig. 5).
Distribution of Candelariella ahtii (●) and C. citrina (■) in the Northern Hemisphere. In colour online.
Additional specimens examined
Russia: Primorye Territory: Khankaisky District, 15 km S from the Komissarovo settlement, Pogranichnyi Range, top of Siniaya Mt, 44°51′37.5″N, 131°42′34″E, 650 m, on soil and other lichens in rocks crevices, 2013, L. S. Yakovchenko 1172 (VLA); ibid., 2014, E. A. Davydov 16310, 16328, 16483 & L. S. Yakovchenko (hb. Davydov & Yakovchenko); Chuguevsky District, Sikhote-Alin' Range, at the right bank of the Egerskaya River between Verkhniaya Breevka and Arkhipovka, Parshivaya Mt, 43°46′03.5″N, 133°48′33.5″E, 480 m, siliceous rock outcrops at the summit, surrounded by Quercus mongolica forest, on soil in rock crevices, 2017, E. A. Davydov 17417 & L. S. Yakovchenko (hb. Davydov & Yakovchenko). Altai Territory: Ust'-Kalmansky District, 3 km W from the Ogni settlement, foothills of the Altai Mts, right bank of the Zemlyanukha River, 51°53′40″N, 83°29′05″E, 390 m, open siliceous outcrops of the top of the hill, SW slope, on soil in rock crevices, 2014, E. A. Davydov 16330, 16481, 16468 & L. S. Yakovchenko (ALTB). Republic of Altai: Kosh-Agachsky District, Altai Mts, Sailjugem range, watershed of Bayan-Chagan and Sarzhemoty Rivers, 4 km S of its junction, mountain rocky steppe, on soil in rock crevices and saxicolous lichens, 49°32′06″N, 88°45′31″E, 2550 m, 2014, E. A. Davydov 16312 & L. S. Yakovchenko (ALTB). Republic of Tuva: Mongun-Taiginsky District, Mongun-taiga massif, left side of the Toolaity River valley, 5 km upstream of the Eski-Toolaity Lake, 50°11′36″N, 90°08′46″E, 2550 m, mountain tundras, stonefield, on soil in rock crevices, 2014, E. A. Davydov 16308 & L. S. Yakovchenko (ALTB); ibid., headwaters of the Orta-Shegetei River, 4.5 km upstream of the Sive-Khol′ Lake, 50°09′10″N, 90°01′21″E, 2480 m, stonefield, on crevices of the rocks, 2014, E. A. Davydov 16315 & L. S. Yakovchenko (ALTB).—China: Xinjiang: Mongol'sky Altai Range, upper reaches of Khara-Belchir-he, west slope of Kara-Balchigtau Mt (3215 m), 46°42ʹ08ʺN, 90°58′29ʺE, 2400 m, Larix sibirica forest, on soil in rock crevices, 2007, E. A. Davydov 16314 (ALTB); Zimunai County, Saur Range, N slope, valley of Ul'kun-Ulyasty near the border with Kazakhstan, 15 km to the north of Mustau peak (3816 m a.s.l.), 40 km S of Zimunai, 47°10ʹ30ʺN, 85°38ʹ28ʺE, 2410 m, degraded alpine meadow along the road, on soil in rock crevices, 2007, E. A. Davydov 16316 (ALTB).—Mongolia: Khentii Province: 28 km SW from Dadal, Shulub-Ula Mt, 48°56′59″N, 111°22′08″E, 1100 m, granite boulders in the mountain steppe, on soil in rock crevices, 2006, L. S. Yakovchenko 1266 (hb. Davydov & Yakovchenko); the same massif, 48°56′42″N, 111°22′03″E, 1066 m, granite boulders in the mountain steppe, on soil in rock crevices, 2006, L. S. Yakovchenko 1267 (hb. Davydov & Yakovchenko).
Candelariella citrina de Lesd.
MycoBank No.: MB 410520
Ann. Cryptog. Exot. 5, 120 (1932); type: USA, New Mexico, Environments de Las Vegas, Thunderbird Ranch, alt.: 1950 m, 5. II. 1929 Arsene Brouard (L-106306) 190850 (UPS—lectotype!).
Candelariella terrigena Räsänen, Anales Soc. Ci. Argent. 128, 137 (1939); type: USA, Colorado, Gregory Canyon near Boulder, ad terram argillaceam, 16 Jul 1931 [1921?], C. C. Plitt (the second piece from the right) (H—lectotype!).
Candelariella terrigena var. placodimorpha Hakul., Arch. Soc. Zool.-Bot. Fenn. ‘Vanamo’ 13, 54 (1958); type: USA, New Mexico, Valencia Co., lava flows and sandstone ledges along San Jose River, 3.5 miles SE of Correo on road to Las Lunas, c. 5500 ft, 27 Mar 1956, S. Shushan & W. A. Weber S6893 (TUR—holotype!).
Additional specimens examined
USA: Nevada: Washoe, Dogskin Mountain, south-eastern end of mountain, open north face of small granite outcrop at top of small ridge, with a few scattered Juniperus osteosperma, Artemisia tridentata and Pinus andersonii, 39.8473°N, 119.7647°W, 1800 m, granite, 2016, J. Hollinger 6993; Lincoln, Hiko Range, Crystal Wash, c. 5.5 km ENE of jct. of Hwys 93 and 25, at foot of N-facing cliff in small rhyolite-tuff canyon, 37.5441°N, 115.1712°W, 1248 m, vertical shaded tuff, 2018, J. Hollinger 6994. California: Siskiyou County, 41.9786°N, 122.4488°W, 755 m, NW of Iron Gate Reservoir, Horseshoe Ranch Wildlife Area, open savannah and woodland with andesite outcrops, on soil and mosses over rocks, March 2017, B. McCune 37341 with NW lichenologists; Orange Co., south slope of Santa Ana Mts, below Silverado near the boundary of Cleveland National Forest, on sandstone rocks at the river, elev. c. 700 m, 1966, R. Santesson 17723 (F69994-S). Arizona: Maricopa, White Tank Mountains, county regional park, habitat on granite, 1975, T. H. Nash 9808 (F69998–S). Texas: Brewster County, Big Bend National Park, low hill just SE of headquarters at Panther Junction, along north-facing hillside among boulders in desert, elev. 3800 ft, 1980, C. M. Wetmore 40838 (F69997-S).
Discussion
Phylogenetic position and diagnostic traits
Candelariella ahtii clusters as sister to morphologically and ecologically similar C. citrina, both having a squamulose thallus with a greenish yellow pulverulent surface, similar type and size of apothecia, and ascospores of similar size, but the latter species has citriform to broadly fusiform ascospores with one or both ends pointed (Westberg Reference Westberg2007c), whereas those of C. ahtii are ellipsoid, or narrowly ellipsoid to broadly ellipsoid, with rounded ends. Candelariella ahtii, in contrast to C. citrina, usually has a cushion-forming thallus of imbricate, large granules or squamules divided by deep cracks, whereas C. citrina rarely forms a continuous crust (Westberg Reference Westberg2007c), although specimens examined from Nevada (J. Hollinger 6993) showed a similar thallus morphology to C. ahtii. In addition, the size of the squamules is smaller (up to 0.5(–0.8) mm long in C. ahtii and up to 0.6(–1.3) mm long in C. citrina); the ascospores with equal maximum length (up to 16 μm) are narrower (up to 5.5(–6) μm in C. ahtii and up to 6.5(–7) μm in C. citrina). Candelariella grimmiae described from Nepal (Poelt & Reddi Reference Poelt and Reddi1969) is similar to C. citrina (Westberg Reference Westberg2007c) and C. ahtii in having a squamulose thallus with a greenish yellow to ginger yellow heavy pulverulent surface, even characterized as ‘sorediate in some areas, but without the formation of distinct sorale’ in the protologue, and occurring on soil over gneiss. The ascospore size given in the protologue is (13.0–)15.0–18.5 × (4.5–)5.0–6.5 μm which is larger than those observed in C. ahtii and C. citrina. Unfortunately, the shape of the ascospores and the shape of the ends, which are key characters in this case, are not given in the protologue. We agree with Westberg (Reference Westberg2007c) that additional material is needed to confirm the independent status of these species. Candelariella grimmiae is distinguished from C. ahtii by its larger thallus of prostrate well-developed squamules with deeply incised ascending margins, whereas C. ahtii usually forms a cushion-forming crust of imbricate granules or squamules divided by cracks into irregular clusters, and the size of the squamules is smaller than given for C. grimmiae. The thallus of thick cushions up to 2 mm thick divided by cracks is also known in the saxicolous C. coralliza (Nyl.) H. Magn. but this has polysporous asci; when sterile the species is distinguished by its thallus composed of deeply incised coralloid squamules, which are even considered to be isidia (Khodosovtsev Reference Khodosovtsev2005). Candelariella ahtii is often found together with C. vitellina s. lat., which also possesses a thallus in the form of a thick pulvinate crust, but differs in its polyspored asci with oblong, smaller, up to 12.5(–14.0) μm long ascospores. Candelariella vitellina s. lat. was often found sterile but could be well distinguished from C. ahtii by its smaller, up to 0.2 mm long squamules with serrated margins and an orange-yellow smooth upper surface. Candelariella ahtii differs from terricolous and muscicolous C. canadensis H. Magn. by its thallus morphology and apothecia with a flattened disc and a permanent, thick thalline margin. Candelariella rosulans (Müll. Arg.) Zahlbr. has a similar apothecial morphology to C. ahtii but is distinguished by its significantly larger ascospores, different thallus morphology and ecological preferences.
Distribution and ecology
The ecology of Candelariella ahtii seems to be quite similar to that of C. citrina (Westberg Reference Westberg2005, Reference Westberg2007c), a related species from the Americas which is common in open habitats at moderate elevations, reaching up to at least 2200 m, but with a wider range of substrata, growing not only on soil and other lichens but also on saxicolous mosses (Westberg Reference Westberg2007c). We have found C. ahtii growing on Montanelia tominii, Rhizoplaca chrysoleuca and R. subdiscrepans only in two locations in the Russian Far East (North-East Asia) and the Altai Mts (Central Asia) and have never seen it on mosses. In addition, we usually found C. ahtii mixed with and on C. vitellina s. lat., on the same rock face in locations at moderate and high elevations. Both species grow on soil in rock crevices although normally Candelariella vitellina s. str. grows on siliceous rock and is less often found on a variety of substrata with the exception of soil (Westberg Reference Westberg2007b).
Morphological and molecular phylogenetic data support the close relationship of C. ahtii and C. citrina. Since all our ITS sequences of C. ahtii are from Asia and C. citrina sequences from NCBI are from North America, our results might be interpreted as an existence of two vicarious species that differ in geographical range and ascospores shape. We cannot deny this possibility despite the limited availability of specimens. Nevertheless, the record of C. citrina from Siberia (Urbanavichus Reference Urbanavichus2010) is considered dubious and should be excluded from the lichen flora of Russia.
Worldwide key to the 8-spored Candelariella s. lat.
All 41 known Candelariella s. lat. species (including two of Protocandelariella) with 8-spored asci are included in the key. Other than names with an asterisk (*), all taxa either have a distinct morphology and/or molecular data, or type material and/or relevant herbarium material studied by the first author in ALTB, F, GZU, H, H-ACH, H-NYL, HIRO, KPABG, LE, NIBR, S, TDZ, TNS, TUR, VBGI, VLA and UPS. Although some little-known species with 8-spored asci have not been studied by us, they are also included. Distribution data is given for each species as suggested by studied herbarium material or reliable literature data.
1 Lichenicolous ………2
Not lichenicolous ……… 3
2(1) Apothecia biatorine, thallus absent, on Candelina submexicana (de Lesd.) Poelt; N America………C. lichenicola M. Westb.
Apothecia lecanorine, thallus absent or present then grey amorphous, on Lecanora populicola (DC.) Duby; Europe, Asia (Caucasus) ……… C. superdistans (Nyl.) Malme
3(1) Thallus distinctly placodioid, on rocks ………4
Thallus indistinct, areolate, subsquamulose, squamulose, rosulate, never with elongated marginal lobes, on various substrata ……… 6
4(3) Lobes strongly convex and short, 1–2 mm long, pruinose in the central part; Europe, Asia (Caucasus, SW Asia) ……… ………C. rhodax Poelt & Vězda
Lobes flattened and long, up to 3–5 mm long, with or without pruina; different regions ……… 5
5(4) With granular areoles in the centre of the thallus, disintegrating into isidious soredia or blastidia, usually pruinose; Europe, Africa, Asia ……… C. medians (Nyl.) A. L. Sm.
Without vegetative propagules, usually epruinose; Europe, Asia………C. senior Poelt
6(3) Apothecia biatorine ……… 7
Apothecia lecanorine or pseudolecanorine ……… 10
7(6) Thallus endolithic; N America ………C. corviniscalensis C. A. Morse & M. Westb.
Thallus apparent, corticolous; various regions ……… 8
8(7) Thallus yellow, crustose; Asia, N America ……… C. biatorina M. Westb.
Thallus grey, squamulose; different regions ……… 9
9(8) Thallus without blastidia, often shiny, conidiophores present on the lower side of the squamules, forming a ±continuous layer; Europe, Africa, Asia, N America, New Zealand ……… P. subdeflexa (Nyl.) Poelt et al.
Thallus with blastidia, matt, conidiophores absent from the lower side of the squamules or poorly developed at the base of the squamules; Europe, Asia, N America ……… P. blastidiata (Yakovch.) D. Liu et al.
10(6) Thallus grey or apparently lacking ………. 11
Thallus yellow ……… 21
11(10) On rocks ………12
On bark, wood, plant debris ………18
12(11) Thallus indistinct, present as a thalline margin which is often partly excluded to indistinct ………. 13
Thallus distinct, areolate, subsquamulose, squamulose, amorphous crustose, thalline margin distinct ………14
13(12) Ascospores (11.0–)12.0–16.0(–16.5) × 4.0–5.0(–6.0) μm, apothecia 0.3–0.55(–0.7) mm wide with yellow thalline margin, often partly excluded; cosmopolitan ………. C. aurella (Hoffm.) Zahlbr.
(grey thallus morphotype)
Ascospores (9.0–)10.0–13.0 × (5.5–)6.0–7.0(–8.0) μm, apothecia 0.5–1.6 mm wide with whitish to greyish thalline margin, suppressed to the lower side of apothecia; N America ……… C. immarginata M. Westb.
14(12) Thallus subsquamulose to squamulose, thick, apothecia with thick, crenulated and convoluted thalline margin ………. 15
Thallus areolate to amorphous crustose, thin, apothecia with thin, even to somewhat crenulated thalline margin ……… 16
15(14) On calcareous rocks or granite, inland, mountain to Arctic-alpine; Europe, Asia, N America, Antarctic ……… ………. C. plumbea Poelt & Vězda
On siliceous rocks, coastal, temperate; E Asia (South Korea) ……… C. hakulinenii S. Y. Kondr. et al.
16(14) Thallus of convex areoles, thalline margin yellow, often partly excluded; cosmopolitan ……… C. aurella (Hoffm.) Zahlbr.
(grey thallus morphotype)
Thallus of flattened areoles or amorphous crustose, thalline margin grey, persistent; various regions ……… 17
17(16) On siliceous rocks, amphibious in splash zone of high mountain streams, inland; C Asia (Russia) ……… ……… C. aquatica Yakovch. et al.
On calcareous rocks, not amphibious or connected with streams, temperate to mountain; Europe, Africa, Asia ……… ……… C. oleaginescens Rondon
18(11) Apothecia large, (0.3–)0.5–1.2(–1.5) mm diam., aggregate. On plant debris or shrubs in steppes and salt marshes; Europe, C Asia ………C. boikoi Khodos. & S. Y. Kondr.
Apothecia smaller, 0.2–0.8(–1.0) mm diam., scattered to aggregate. On bark, wood in various habitats; various regions ……… 19
19(18) Thallus uniformly composed of crowded spherical granules, unbranched to coralloid; Europe, Asia ……… ……… C. viae-lacteae G. Thor & V. Wirth
Thallus indistinct and seen only at the base of apothecia to amorphous crustose or areolate; various regions ……… 20
20(19) Thallus indistinct to areolate, of convex areoles, thalline margin entirely yellow, often partly excluded, proper margin of aurella-type, of elongated, thick-walled cells forming a compact stipe penetrating through algal layer; on wood; cosmopolitan .………C. aurella (Hoffm.) Zahlbr.
(grey thallus morphotype)
Thallus indistinct to amorphous crustose, thalline margin yellow, often greyish in the outer side, persistent, proper margin of aggregata-type, of more or less isodiametrical, thin-walled cells not forming a compact stipe; on bark, rarely on wood; Europe, Asia, N America .………C. antennaria Räsänen
21(10) Thallus with vegetative propagules ……… 22
Thallus without vegetative propagules ………27
22(21) Thallus squamulose to subsquamulose-granulate ………. 23
Thallus of minute areoles ………. 25
23(22) Soredia laminal, formed in crateriform soralia; thallus distinctly squamulose, squamules up to 1(–2) mm wide, rosette with loosely attached margins; corticolous, temperate; Europe, Asia (Caucasus) ……… C. reflexa Poelt & Vězda
Soredia marginal, dispersed inward or to the lower side of the subsquamules; thallus subsquamulose to granulose, subsquamules up to 0.5 mm wide, ascending from the one margin ………24
24(23) Subsquamules 0.2–0.5 mm wide; soredia (–20)30–50(–60) μm diam., dispersed to the lower side of the subsquamules; thallus never totally dissolved into sorediate crust; corticolous and muscicolous; high mountains; E Asia and S Asia (Nepal, Taiwan) ……… *C. sorediosa Poelt & Reddi
Subsquamules (–0.05)0.06–0.2(–0.3) mm wide; soredia (–30)40–70(–80) μm diam., dispersed to the lower side of the subsquamules or inward, soon forming a ±continuous sorediate crust; corticolous, temperate, mountain; Europe, S Asia (China) ………C. rubrisoli D. Liu & Hur
25(22) Soredia 20–60(–70) μm diam., apothecia up to 0.25(–0.3) mm diam., ascospores (11.0–)11.2–13.3–15.4(–17.7) × (3.8–)4.2–5.0–5.9(–7.2) μm, corticolous, temperate; Asia (Caucasus, Japan), N America, Australia ……….. ……… C. xanthostigmoides (Müll. Arg.) R. W. Rogers
Soredia 20–50 μm diam., apothecia up to 0.55 mm diam., ascospores (12.0–)14.0–21.0 × (4.5–)5.0–8.0(–10.5) μm, corticolous; Southern Hemisphere ……… 26
26(25) Soredia c. 25 μm diam., thalline margin persistent and non-sorediate, asci 8-spored, ascospores simple, fusiform to rhomboidal with rounded ends, (14.0–)18.0–21.0 × 5.0–6.0 μm; Africa (Réunion) ……… C. flavosorediata Kalb & Aptroot
Soredia 20–50 μm diam., thalline margin soon disappearing and becoming granular-sorediate, asci 4–8-spored, ascospores simple to 1(–2)-septate, elongate to broadly ellipsoid, (12.0–)14.0–19.0(–21.0) × (4.5–)5.0–8.0(–10.5) μm; S America (Chile) ………. C. magellanica Etayo
27(21) On bark and wood ………. 28
On rocks, soil, mosses, plant debris and other lichens ……… 30
28(27) Thallus uniformly granular, on bark and wood; N America ……… C. deppeanae M. Westb.
Thallus areolate, squamulose to indistinct, saxicolous species occasionally growing on bark or wood; various regions……… 29
29(28) Thallus areolate, small granulate to indistinct, apothecia up to 0.55(–0.7) mm wide, with somewhat convex disc and partly excluded thalline margin, ascospores mostly narrowly ellipsoid; cosmopolitan ………. C. aurella (Hoffm.) Zahlbr.
Thallus mostly squamulose, sometimes indistinct, apothecia up to 1.2(–2.2) mm wide, with flat to somewhat convex disc and persistent thalline margin, ascospores mostly oblong; Europe, Asia, N America, Australia ……… C. rosulans (Müll. Arg.) Zahlbr.
30(27) On rocks ………31
On plant debris, soil, mosses and other lichens ………41
31(30) On calcareous rocks ………. 32
On siliceous rocks ……… 35
32(31) Thallus well developed, squamulose ……… 33
Thallus poorly developed, areolate to indistinct ……… 34
33(32) Thallus shiny, cortex pseudoparenchymatous, 25–60 μm thick, gelatinous epicortex present; Asia, N America ……… ……… C. kansuensis H. Magn.
Thallus matt, cortex indistinct to pseudoparenchymatous, up to 25 μm thick, gelatinous epicortex absent; Europe, Asia, N America, Australia ………C. rosulans (Müll. Arg.) Zahlbr.
34(32) Apothecia 0.3–0.55(–0.7) mm diam., rounded, with partly excluded thalline margin and soon somewhat convex disc, always on calcareous rocks; cosmopolitan ……… C. aurella (Hoffm.) Zahlbr.
Apothecia 0.4–1.2(–2.2) mm diam., rounded to wavy, with persistent thalline margin and usually flattened disc, rarely on calcareous rocks; Europe, Asia, N America, Australia ……… C. rosulans (Müll. Arg.) Zahlbr.
35(31) Thallus usually of deeply incised squamules with finger-like lobes, scattered or forming effigurate to minutely shrubby thalli; N America ……… C. corallizoides M. Westb.
Thallus otherwise; various regions ……… 36
36(35) Ascospores acicular, (30.5–)34.5–48.0(–57.0) × 3–4(–5) μm; Asia, N America ………C. spraguei (Tuck.) Zahlbr.
Ascospores much smaller, ellipsoid to oblong; various regions ………37
37(36) Thallus greenish yellow to pale yellow ………38
Thallus yolk yellow to orange-yellow ……… 39
38(37) Apothecia immersed, pseudolecanorine, ascospores narrowly to broadly ellipsoid, (8.0–)11.0–17.0 × 3.5–5.0(–7.0) μm; N America ……… C. clarkii E. Tripp & Lendemer
Apothecia sessile, lecanorine with strongly convex, globose disc and soon excluded thalline margin, ascospores narrowly ellipsoid, 15–22 × 4.5–6 μm; N America ……… C. californica M. Westb.
39(37) Thallus of flattened, rounded, peltate squamules with smooth upper surface; N America, S America ……… ……… C. complanata M. Westb.
Thallus indistinct or of convex areoles/lobate squamules with smooth to pulverulent upper surface; various regions ……… 40
40(39) Thallus surface pulverulent, ascospores 8–13(–15) × 4–5(–6) μm, asci 35–55 × 11–16 μm, hymenium 45–60 μm tall; Australia ……… C. australiensis McCarthy & Elix
Thallus surface smooth to coarse, ascospores (11–)14–22(–25) × (4–)5–6(–8) μm, asci 40–64 × 12–22 μm, hymenium 50–90 μm tall; Europe, Asia, N America, Australia ……… C. rosulans (Müll. Arg.) Zahlbr.
41(30) Ascospores acicular, (30.5–)34.5–48.0(–57.0) × 3–4(–5) μm, saxicolous species occasionally growing on soil and mosses in rock crevices; Asia, N America ………. C. spraguei (Tuck.) Zahlbr.
Ascospores much smaller, ellipsoid to oblong, always on plant debris, soil, mosses, other lichens; various regions ……… 42
42(41) Apothecia numerous and dominant, thallus poorly developed, of scattered granules/small squamules, 0.1–0.35 mm wide or reduced to the margin of apothecia ………43
Apothecia present or not, thallus well developed, squamulose of scattered to crowded and imbricate granules/squamules ………. ………. 45
43(42) Apothecia large, up to 2.0 mm diam., finally lobate, with a thick, persistent, raised thalline margin, proper margin of aurella-type, ascospores (18.0–)20.0–28.0(–32.0) × 5.0–6.5(–7.8) μm, on plant debris, soil; Arctic-alpine: Europe, Asia (Caucasus) ……… C. commutata Otte & M. Westb.
Apothecia smaller, 0.3–0.6(–1) mm diam., rounded with a thin persistent to excluded thalline margin, proper margin of aggregata-type, ascospores (9.0–)11.0–19.0(–24.0) × 4.0–7.5(–9.0) μm; various regions ……… 44
44(43) Ascospores (9.0–)11.0–13.5–16.0(–19.0) × (4.0–)4.5–6.0–7.5(–9.0) μm, phylogenetic sister species to C. aurella s. lat.; Antarctic ……… C. ruzgarii Halıcı et al.
Ascospores (10.5–)13.0–19.0(–24.0) × (4.0–)4.5–6.0(–7.5) μm, phylogenetically outside of C. aurella s. lat. group, on mosses, plant debris; Europe, Asia, N America ……… C. aggregata M. Westb.
45(42) Thallus squamulose, rosette-like, granulose, of scattered to converged granules or subsquamules/squamules but not forming a pulvinate crust ……… 46
Thallus cushion-forming, of imbricate, ±uniform granules/subsquamules/squamules forming a thick pulvinate crust divided by cracks ………. 49
46(45) Upper surface dark yellow, smooth to pulverulent, apothecia with convex disc and indistinct thalline margin when mature, Arctic-alpine; Europe, E Asia and S Asia (Nepal, Taiwan), N America……… C. canadensis H. Magn.
Upper surface citrine green, greenish yellow, pale yellow, ginger yellow, heavy pulverulent, apothecia with flattened disc and persistent thalline margin; various regions ……… 47
47(46) Ascospores ovoid to citriform, broadly fusiform or teardrop-shaped with at least one end pointed; mostly N America, rarely S America ………C. citrina de Lesd.
Ascospores ellipsoid to narrowly ellipsoid, rarely broadly ellipsoid without pointed ends; various regions ……… 48
48(47) Squamules large, 0.7–1.0 mm long, deeply incised to lobate, prostrate with finally ascending (almost vertical) margins; E Asia and S Asia (Nepal, Taiwan)……… *C. grimmiae Poelt & Reddi
Squamules (0.15–)0.25–0.35–0.5(–0.8) mm long, non-incised to shallowly incised, with adpressed margins; C Asia, NE Asia (Russia) ……… C. ahtii Yakovch.
49(45) Thallus yellow, yellow-orange, of small, 0.06–0.1 mm wide, ecorticate or corticated granules, on mosses and on other lichens; Antarctic………. C. flava (C. W. Dodge & G. E. Baker) Gastello & Nimis
Thallus of citrine green, greenish yellow, pale yellow, pulverulent, large, 0.15–0.8(–1.3) mm long granules or subsquamules/squamules, on soil in rock crevices, rarely on other lichens and mosses; different regions ……… 50
50(49) Ascospores ovoid to citriform, broadly fusiform or teardrop-shaped with at least one end pointed, squamules up to 0.6(–1.3) mm long; mostly N America, rarely S America ……… C. citrina de Lesd.
Ascospores ellipsoid to narrowly ellipsoid, rarely broadly ellipsoid, without pointed ends, squamules up to 0.5(–0.8) mm long; C Asia, NE Asia ……… C. ahtii Yakovch.
Acknowledgements
We are grateful to Prof. Teuvo Ahti (H) for his hospitality and omnifarious help during the visits of LY and ED to Helsinki; to Dr M. Westberg (UPS) for the opportunity for LY to sequence material from Mongolia and the Russian Far East and for making an assumption on the independent status of C. ahtii; to Dr Christian Printzen (FR) for the opportunity to sequence part of the material in his laboratory and for arranging loans of specimens to LY; to the curators of ALTB, F, GZU, H, H-ACH, H-NYL, HIRO, KPABG, LE, NIBR, S, TDZ, TNS, TUR, VBGI, VLA and UPS for their assistance during the visits of LY and ED; to Prof. B. McCune and Dr J. Hollinger for providing us with additional American material. We acknowledge Dr Pavel V. Golyakov (Tigirek State Natural Reserve, Barnaul) and Alexandr V. Fedonyuk (Botanical Garden Institute FEB RAS, Vladivostok) for organizing field excursions as well as Alexander S. Yakovchenko for technical help. The research of LY was carried out within the state assignment of the Ministry of Science and Higher Education of the Russian Federation (theme no. 124012400285-7).
Author ORCIDs
Lidia S. Yakovchenko, 0000-0002-4342-7771; Evgeny A. Davydov, 0000-0002-2316-8506.
Competing Interests
The authors declare none.
